Porated cartilage products

Patent metadata

Field	Value
Publication number	US-10874763-B2
Application number	US-201414464123-A
Country	US
Kind code	B2
Filing date	Aug 20, 2014
Priority date	Jul 11, 2012
Publication date	Dec 29, 2020
Grant date	Dec 29, 2020

Abstract

This invention provides porated cartilage products, methods of producing porated cartilage products, and methods of treating subjects by administering cartilage products. Optionally, the cartilage products are sized, porated, and digested to provide a flexible cartilage product. Optionally, the cartilage products comprise viable chondrocytes, bioactive factors such as chondrogenic factors, and a collagen type II matrix. Optionally, the cartilage products are non-immunogenic.

First claim

What is claimed is:

1. A cryopreserved cartilage product comprising cryopreserved partially-digested, natural cartilage, wherein the cryopreserved partially-digested natural cartilage comprises a natural cartilage collagen matrix, wherein the cryopreserved partially-digested natural cartilage comprises viable cells native to the natural cartilage, wherein at least 50% of the native cells present in the natural cartilage collagen matrix before cryopreservation are viable in the cryopreserved cartilage product.

2. The cryopreserved cartilage product of claim 1 , wherein the natural cartilage collagen matrix is a fragmented cartilage collagen matrix.

3. The cryopreserved cartilage product of claim 2 , wherein the fragmented cartilage collagen matrix comprises glycine-terminated collagen fragments.

4. The cryopreserved cartilage product of claim 1 , wherein the cryopreserved partially-digested natural cartilage is enzyme digested.

5. The cryopreserved cartilage product of claim 4 , wherein said enzyme is a proteinase.

6. The cryopreserved cartilage product of claim 1 , wherein the viable cells are chondrocytes.

7. The cryopreserved cartilage product of claim 1 , wherein the cartilage comprises hyaline cartilage.

8. The cryopreserved cartilage product of claim 1 , wherein the cartilage product is devoid of subchondral bone or, calcified cartilage.

9. The cryopreserved cartilage product of claim 1 , wherein the cartilage has a thickness of less than about 3 mm.

10. The cryopreserved cartilage product of claim 9 , wherein the wherein the cartilage has a thickness of about 0.5 mm to about 2 mm or about 1 mm to about 1.5 mm.

11. The cryopreserved cartilage product of claim 1 , wherein the cartilage product comprises chondrogenic factors native to the cartilage.

12. A cartilage product comprising natural cartilage, wherein the natural cartilage comprises a natural cartilage collagen matrix wherein the natural cartilage contains viable cells native to the natural cartilage, wherein at least 50% of the cells native to the natural cartilage are viable, and wherein the natural cartilage is minced.

13. The cartilage product of claim 5 , wherein said proteinase is collagenase or collagenase II.

14. The cryopreserved cartilage product of claim 1 , wherein at least 60% of the native cells present in the natural cartilage collagen matrix before cryopreservation are viable in the cryopreserved cartilage product.

15. The cryopreserved cartilage product of claim 1 , wherein at least 70% of the native cells present in the natural cartilage collagen matrix before cryopreservation are viable in the cryopreserved cartilage product.

16. The cartilage product of claim 12 , wherein the minced natural cartilage comprises at least 60% viable native cells compared to the total number of cells present in the minced natural cartilage.

17. The cartilage product of claim 12 , wherein the minced natural cartilage comprises at least 70% viable native cells compared to the total number of cells present in the minced natural cartilage.

Assignees

• Osiris Therapeutics Inc

Inventors

• Yoo Dana
• Kuang Jin-Qiang
• Paden Jaime
• Maxson Scott
• Danilkovitch Alla
• Lopez Erasmo
• Tom Samson

Classifications

• A61K38/014

  from connective tissue peptides, e.g. gelatin, collagen · CPC title

• A61L27/3852

  Cartilage, e.g. meniscus · CPC title

• A61K35/32

  Bones; Osteocytes; Osteoblasts; Tendons; Tenocytes; Teeth; Odontoblasts; Cartilage; Chondrocytes; Synovial membrane · CPC title

• A61L27/3691

  characterised by physical conditions of the treatment, e.g. applying a compressive force to the composition, pressure cycles, ultrasonic/sonication or microwave treatment, lyophilisation · CPC title

• A61L2300/414

  Growth factors · CPC title