Directed stem cell recruitment

Patent metadata

Field	Value
Publication number	US-10874718-B2
Application number	US-201715640063-A
Country	US
Kind code	B2
Filing date	Jun 30, 2017
Priority date	May 9, 2008
Publication date	Dec 29, 2020
Grant date	Dec 29, 2020

Abstract

The invention is directed to methods of inducing cell recruitment and tissue regeneration at a target site in a subject. It is also based, in part, on the discovery that a subject's own biologic resources and environmental conditions can be used for in situ tissue regeneration and thereby reduce or eliminate the need for donor cell procurement and ex vivo manipulation of such donor cells. Methods are disclosed for recruitment of a subject's own stem cells to a target region by inducing a sustained positive pressure at a target site, such as the kidney, thereby increasing the number of pluripotent cells capable of differentiating to regenerate the target tissue.

First claim

The invention claimed is:

1. A method of kidney regeneration comprising:
injecting a substantially cell-free biocompatible substance comprising collagen under pressure into a target site in a kidney to create a hyperbaric environment at the target site;
maintaining positive pressure in a range of about 5 cm H 2 O to about 70 cm H 2 O at the target site for at least one hour due to the viscosity of the substance or collagen swelling-during which time the hyperbaric environment recruits tissue-specific progenitor cells to the target site; and
wherein the tissue-specific progenitor cells recruited to the target site undergo differentiation to produce new glomeruli structures within the kidney.

2. The method of claim 1 , wherein the biocompatible and biodegradable substance-further comprises a substantially cell-free polymeric biocompatible and biodegradable collagen substance.

3. The method of claim 2 , wherein the collagen biocompatible and biodegradable substance is a collagen solution having a collagen concentration from about 2 mg/mL to about 5 mg/mL.

4. The method of claim 3 , wherein the collagen solution thermogels at body temperature and the step of delivering the biocompatible and biodegradable substance further comprises injecting the collagen solution in a chilled state as a liquid and the step of maintaining positive pressure at the target site further comprises gelling the substance following injection.

5. The method of claim 1 , wherein the biocompatible and biodegradable substance further comprises a hydrogel.

6. The method of claim 3 , wherein the step of delivering the collagen solution into the target site comprises injecting the substance into the target site and monitoring an injection pressure such that a hyperbaric environment is attained at the target site.

7. The method of claim 1 , wherein the step of delivering the biocompatible substance into the target site comprises surgically implanting the substance at the target site.

Assignees

• Univ Wake Forest Health Sciences

Inventors

• Yoo James
• Lee Sang Jin
• Atala Anthony
• Van Dyke Mark

Classifications

• A61L27/52

  Hydrogels or hydrocolloids · CPC title

• A61L2300/41

  Anti-inflammatory agents, e.g. NSAIDs · CPC title

• A61L27/54

  Biologically active materials, e.g. therapeutic substances {(A61L27/227 takes precedence)} · CPC title

• A61K38/4886

  Metalloendopeptidases (3.4.24), e.g. collagenase · CPC title

• A61L2400/06

  Flowable or injectable implant compositions · CPC title