Stable inoculant compositions and methods for producing same

Patent metadata

Field	Value
Publication number	US-10874109-B2
Application number	US-201916275513-A
Country	US
Kind code	B2
Filing date	Feb 14, 2019
Priority date	Dec 28, 2015
Publication date	Dec 29, 2020
Grant date	Dec 29, 2020

Abstract

The present disclosure provides non-aqueous inoculant compositions and methods for enhancing the survival and/or stability of microbial spores in an inoculant composition. In some embodiments, inoculant compositions of the present disclosure comprise microbial spores, one or more dispersants, one or more protectants and a non-aqueous liquid carrier.

First claim

That which is claimed:

1. A non-aqueous inoculant composition, comprising:
microbial spores;
a polyethylene glycol;
a vinylpyrrolidone polymer; and
a polyethylene glycol-polypropylene glycol copolymer.

2. The non-aqueous inoculant composition of claim 1 , wherein said microbial spores comprise about 0.1% to about 25% (by weight) of said inoculant composition.

3. The non-aqueous inoculant composition of claim 1 , wherein said microbial spores comprise about 5% to about 25% (by weight) of said inoculant composition.

4. The non-aqueous inoculant composition of claim 1 , wherein said microbial spores comprise about 10 1 to about 10 15 colony-forming units per gram and/or milliliter of said inoculant composition.

5. The non-aqueous inoculant composition of claim 1 , wherein said microbial spores comprise Bacillus spores, Gliocladium spores, Glomus spores, Metarhizium spores, Penicillium spores and/or Trichoderma spores.

6. The non-aqueous inoculant composition of claim 1 , wherein said polyethylene glycol comprise about 75% to about 95% (by weight) of said inoculant composition.

7. The non-aqueous inoculant composition of claim 1 , wherein said polyethylene glycol is PEG 200, PEG 300 or PEG 400.

8. The non-aqueous inoculant composition of claim 1 , wherein said one or more vinylpyrrolidone polymers comprises about 0.001% to about 1% (by weight) of said composition.

9. The non-aqueous inoculant composition of claim 1 , wherein said one or more vinylpyrrolidone polymers comprises about 1% to about 10% (by weight) of said composition.

10. The non-aqueous inoculant composition of claim 1 , further comprising one or more sugar alcohols.

11. The non-aqueous inoculant composition of claim 10 , wherein said one more sugar alcohols comprises about 0.001% to about 1% (by weight) of said inoculant composition.

12. The non-aqueous inoculant composition of claim 10 , wherein said one more sugar alcohols comprises about 1% to about 10% (by weight) of said inoculant composition.

13. The non-aqueous inoculant composition of claim 1 , further comprising one or more disaccharides.

14. The non-aqueous inoculant composition of claim 13 , wherein said one or more disaccharides comprises about 0.001% to about 1% (by weight) of said inoculant composition.

15. The non-aqueous inoculant composition of claim 13 , wherein said one or more disaccharides comprises about 1% to about 10% (by weight) of said inoculant composition.

16. The non-aqueous inoculant composition of claim 1 , wherein said polyethylene glycol-polypropylene glycol copolymer comprises about 0.01% to about 25% (by weight) of said inoculant composition.

17. The non-aqueous inoculant composition of claim 1 , wherein said polyethylene glycol-polypropylene glycol copolymer comprises about 0.1% to about 5% (by weight) of said inoculant composition.

18. A non-aqueous liquid inoculant composition, comprising:
microbial spores comprising about 5% to about 25% (by weight) of said inoculant composition; and
a non-aqueous carrier comprising about 75% to about 95% (by weight) of said inoculant composition, said non-aqueous carrier comprising, at least one polyethylene glycol-polypropylene glycol copolymer, at least one polyethylene glycol and at least one vinylpyrrolidone polymer.

19. A method comprising contacting a plant growth medium, a plant propagation material and/or a plant with the non-aqueous inoculant composition of claim 1 .

20. A composition comprising a plant seed that is at least partially coated with the non-aqueous inoculant composition of claim 1 .

21. A method comprising introducing the composition of claim 20 into a plant growth medium.

Assignees

• Novozymes Bioag As
• Monsanto Technology Llc

Inventors

• Greenshields Dave
• Martin Vincent
• Selness Shaun Raj
• Han Hui

Classifications

• A01G7/06

  Treatment of growing trees or plants, e.g. for preventing decay of wood, for tingeing flowers or wood, for prolonging the life of plants · CPC title

• A01G22/00

  Cultivation of specific crops or plants not otherwise provided for · CPC title

• A01N63/36 Primary

  Penicillium · CPC title

• C12N11/10

  the carrier being a carbohydrate · CPC title

• C12N1/04

  Preserving or maintaining viable microorganisms (immobilised microorganisms C12N11/00) · CPC title