Compositions for detecting mutant anaplastic lymphoma kinase in lung cancer

Patent metadata

Field	Value
Publication number	US-10870892-B2
Application number	US-201815973718-A
Country	US
Kind code	B2
Filing date	May 8, 2018
Priority date	Apr 14, 2006
Publication date	Dec 22, 2020
Grant date	Dec 22, 2020

Abstract

Novel gene deletions and translocations involving chromosome 2 resulting in fusion proteins combining part of Anaplastic Lymphoma Kinase (ALK) kinase with part of a secondary protein have been identified herein in human solid tumors, e.g. non-small cell lung carcinoma (NSCLC). Secondary proteins include Echinoderm Microtubule-Associated Protein-Like 4 (EML-4) and TRK-Fusion Gene (TFC). The EML4-ALK fusion protein, which retains ALK tyrosine kinase activity, was confirmed to drive the proliferation and survival of NSCLC characterized by this mutation. The invention therefore provides, in part, isolated polynucleotides and vectors encoding the disclosed mutant ALK kinase polypeptides, probes for detecting it, isolated mutant polypeptides, recombinant polypeptides, and reagents for detecting the fusion and truncated polypeptides. The disclosed identification of this new fusion protein enables methods for screening for compounds that inhibit the proteins, and methods for inhibiting the progression of a cancer characterized by the mutant polynucleotides or polypeptides.

First claim

What is claimed is:

1. A composition, comprising
a biological sample from a patient having lung cancer, and
a reagent that detects a mutant Anaplastic Lymphoma Kinase (ALK) polypeptide,
wherein the mutant ALK polypeptide has ALK kinase activity and is an Echinoderm Microtubule-Associated Protein-Like 4 (EML4)-ALK fusion polypeptide comprising an amino acid sequence having at least 95% identity to an amino acid sequence which comprises residues 1-222 of SEQ ID NO: 3 and residues 1116-1383 of SEQ ID NO: 5,
and
wherein the reagent comprises an antibody that binds to the mutant ALK polypeptide.

2. The composition of claim 1 , wherein said lung cancer is non-small cell lung cancer (NSCLC).

3. The composition of claim 1 , wherein said biological sample is a serum, pleural effusion, or tissue biopsy sample.

4. The composition of claim 1 , wherein the sample is a paraffin-embedded lung tissue sample comprising tumor cells.

5. The composition of claim 1 , wherein the antibody is conjugated with a detectable label.

6. The composition of claim 1 , wherein said mutant ALK polypeptide comprises residues 1116-1383 of SEQ ID NO: 5.

7. A composition comprising
a biological sample from a patient having lung cancer, and
a reagent that detects a mutant ALK polypeptide,
wherein the mutant ALK polypeptide has ALK kinase activity and is an EML4-ALK fusion polypeptide comprising an amino acid sequence having at least 95% identity to SEQ ID NO: 1 or SEQ ID NO: 18, and
wherein the reagent comprises an antibody that binds to the mutant ALK polypeptide.

8. The composition of claim 7 , wherein said lung cancer is non-small cell lung cancer (NSCLC).

9. The composition of claim 7 , wherein said biological sample is a serum, pleural effusion, or tissue biopsy sample.

10. The composition of claim 7 , wherein the sample is a paraffin-embedded lung tissue sample comprising tumor cells.

11. The composition of claim 7 , wherein the antibody is conjugated with a detectable label.

12. The composition of claim 7 , wherein said mutant ALK polypeptide comprises residues 1116-1383 of SEQ ID NO: 5.

Assignees

• Cell Signaling Technology Inc

Inventors

• Rikova Klarisa
• Haack Herbert
• Sullivan Laura
• Guo Ailan
• Possemato Anthony
• Macneill Joan
• Gu Ting-Lei
• Yu Jian

Classifications

• G01N33/5758

  involving compounds serving as markers for tumours, cancers or neoplasias, e.g. cellular determinants, receptors, heat shock/stress proteins, A-protein, oligosaccharides or metabolites · CPC title

• G01N33/5752

  of the lungs · CPC title

• C12Q1/6886 Primary

  for cancer (immunoassay for cancer G01N33/575) · CPC title

• C07K14/47 Primary

  from mammals · CPC title

• C12N9/12

  transferring phosphorus containing groups, e.g. kinases (2.7) · CPC title