Genetic reduction of male fertility in plants

Patent metadata

Field	Value
Publication number	US-10870860-B2
Application number	US-201816194706-A
Country	US
Kind code	B2
Filing date	Nov 19, 2018
Priority date	Mar 13, 2012
Publication date	Dec 22, 2020
Grant date	Dec 22, 2020

Abstract

A nuclear maize gene is mutated to result in dominant male sterility. Two mutants are provided which impact signal peptide processing. Restoration constructs and methods are provided for use of the dominant male-sterile mutants in producing hybrid seed for male-sterile hybrid plants. Other signal-peptide modifications are provided. Orthologs of the wild type or mutated gene in various species are provided.

First claim

The invention claimed is:

1. A DNA construct comprising an isolated regulatory region of a maize Ms44 gene, wherein the Ms44 regulatory region is operably-linked to a heterologous polynucleotide of interest, wherein the Ms44 regulatory region initiates transcription of the heterologous polynucleotide of interest in a plant cell.

2. The DNA construct of claim 1 , wherein the Ms44 regulatory region initiates transcription in a plant cell in a male-tissue preferred manner.

3. The DNA construct of claim 1 , wherein the Ms44 regulatory region comprises the regulatory region within each of SEQ ID NO: 9 or 13.

4. The DNA construct of claim 1 , wherein the heterologous polynucleotide of interest encodes a marker.

5. The DNA construct of claim 1 , wherein the heterologous polynucleotide of interest encodes a gene product that confers drought tolerance, cold tolerance, herbicide tolerance, pathogen resistance, or insect resistance to the plant.

6. The DNA construct of claim 1 , wherein the Ms44 regulatory region initiates transcription of the heterologous polynucleotide of interest in a monocot plant cell.

7. The DNA construct of claim 6 , wherein the Ms44 regulatory region initiates transcription of the heterologous polynucleotide of interest in a maize plant cell.

8. The DNA construct of claim 1 , wherein the Ms44 regulatory region initiates transcription of the heterologous polynucleotide of interest in a dicot plant cell.

9. A vector or expression cassette, each comprising the DNA construct of claim 1 .

10. A plant cell comprising the DNA construct of claim 1 .

11. The plant cell of claim 10 , wherein the plant cell is a monocot plant cell.

12. The plant cell of claim 10 , wherein the plant cell is a dicot plant cell.

Assignees

• Pioneer Hi Bred Int
• Du Pont

Inventors

• Albertsen Marc C
• Fox Tim
• Leonard April L
• Li Bailin
• Loveland Brian R
• Trimnell Mary

Classifications

• Y02A40/146

  Genetically Modified [GMO] plants, e.g. transgenic plants · CPC title

• C12N15/8289 Primary

  Male sterility · CPC title

• C12N15/8271

  for stress resistance, e.g. heavy metal resistance · CPC title

• C12N15/8231 Primary

  Male-specific, e.g. anther, tapetum, pollen · CPC title

• C12N15/8221

  Transit peptides · CPC title