Vessel for growth of biological entities

Patent metadata

Field	Value
Publication number	US-10870822-B2
Application number	US-201515304313-A
Country	US
Kind code	B2
Filing date	Apr 9, 2015
Priority date	Apr 17, 2014
Publication date	Dec 22, 2020
Grant date	Dec 22, 2020

Abstract

A 5-liter bioreactor vessel enables a substantial increase in the volume of biological media that can be cultivated in the vessel compared to conventional designs. Moreover, when agitated at 1.5× the shaking frequency of a conventional 3-liter bioreactor (i.e., 90 rpm versus 60 rpm), the 5-liter vessel achieves a 19% increase in cell aeration without exceeding the maximum shear stress limit for cell viability. The 5-liter vessel optionally includes a plurality of internal baffles configured to disrupt the liquid vortex and reduce the maximum shear stress transferred to biomedia contained within the vessel.

First claim

We claim:

1. A vessel for culturing cells comprising:
a vessel main body defined by a bottom having a rounded edge that is integral with a conically-shaped sidewall that tapers inward to a top surface;
two or more interior baffles configured to disrupt a liquid vortex of a liquid media contained within the vessel during agitation of the liquid media; and
an integral elongate annular neck extending upwardly from the top surface to a vessel opening, wherein
the neck is joined to the vessel main body via a tapered transition area, and
wherein the baffles extend along the conically-shaped sidewall, along the rounded edge to the bottom, and radially inward along the bottom, the baffles each comprising a v-shaped cross-section defined by sidewalls separated by an angle of 80° to 110°, and wherein the baffles extend radially inward from and downward from the rounded edge to the bottom of the vessel at an angle between 5° and 30°.

2. The vessel according to claim 1 , wherein the top surface is substantially horizontal.

3. The vessel according to claim 1 , wherein the vessel main body has a volume of at least 5 liters.

4. The vessel according to claim 1 , wherein the vessel comprises a unitary part.

5. The vessel according to claim 1 , wherein the vessel has a hydrophilic inner surface.

6. The vessel according to claim 1 , wherein the neck diameter is less than a diameter of the vessel opening.

7. The vessel according to claim 1 , wherein the neck diameter is at least 30% of a maximum vessel diameter.

8. The vessel according to claim 1 , where the baffles are raised with respect to an inner surface of the vessel.

9. The vessel according to claim 1 , wherein the neck further comprises a pour trough.

10. The vessel according to claim 1 , wherein the baffles extend radially inward from the rounded edge to the bottom of the vessel at an angle of about 15 degrees.

11. A method of cultivating cells, comprising:
introducing a culture medium into the vessel of claim 1 ;
introducing at least one selected cell line into the vessel; and
agitating the vessel at a frequency of greater than 60 rpm.

12. The method according to claim 11 , wherein the frequency is about 90 rpm.

Assignees

• Corning Inc

Inventors

• Kenney David Alan
• Kladias Nikolaos Pantelis
• Kwei Shang-Pin
• Rammohan Aravind Raghavan
• Wall Joseph Christopher
• Youngbear Kathy Marie

Classifications

• B01F35/53

  characterised by the configuration of the interior, e.g. baffles for facilitating the mixing of components · CPC title

• B01F29/322

  of two or more containers supported for simultaneous mixing, e.g. for bottles in crates · CPC title

• B01F29/40221

  provided with baffles, plates or bars on the wall or the bottom · CPC title

• C12M23/08 Primary

  Flask, bottle or test tube · CPC title

• C12M27/20

  Baffles; Ribs; Ribbons; Auger vanes · CPC title