Methods and compositions related to soluble monoclonal variable lymphocyte receptors of defined antigen specificity

Patent metadata

Field	Value
Publication number	US-10870692-B2
Application number	US-201916265437-A
Country	US
Kind code	B2
Filing date	Feb 1, 2019
Priority date	Aug 2, 2006
Publication date	Dec 22, 2020
Grant date	Dec 22, 2020

Abstract

Disclosed are compositions and methods related to variable lymphocyte receptors (VLRs). More particularly, disclosed are a variety of antigen specific polypeptides, including soluble, monoclonal, and multivalent forms, as well as methods of using the polypeptides, antibodies that bind the antigen specific polypeptides, and nucleic acids, vectors and expression systems that encode the polypeptides. Antigen specific polypeptides that selectively bind pathogens, like anthrax, and carbohydrates, like blood group determinants, are specifically disclosed.

First claim

What is claimed is:

1. A purified multimer comprising a plurality of soluble, monoclonal antigen specific polypeptides, wherein each antigen specific polypeptide comprises
a. a N-terminal leucine rich repeat (LRRNT),
b. one or more internal leucine rich repeats (LRRs),
c. a C-terminal leucine rich repeat (LRCCT), and
d. a connecting peptide, wherein the connecting peptide comprises an alpha helix.

2. The multimer of claim 1 , comprising up to ten antigen specific polypeptides.

3. The multimer of claim 1 , wherein the antigen specific polypeptides bind a target protein.

4. The multimer of claim 1 , wherein the antigen specific polypeptides bind a target carbohydrate.

5. The multimer of claim 1 , wherein the antigen specific polypeptides bind a target pathogen.

6. The multimer of claim 2 , wherein the multimer is a multivalent multimer that binds to more than one target.

Assignees

• Uab Res Found

Inventors

• Cooper Max D
• Herrin Brantley R
• Alder Matthew N

Classifications

• C07K16/1145

  Env proteins, e.g. gp41, gp110/120, gp160, V3, principal neutralising domain [PND] or CD4-binding site · CPC title

• C07K16/108

  Orthomyxoviridae (F), e.g. influenza virus · CPC title

• C07K16/1278 Primary

  Bacillus (G) · CPC title

• C07K2317/14

  Specific host cells or culture conditions, e.g. components, pH or temperature · CPC title

• A61P31/18

  for HIV · CPC title