Bioactive delivery vehicles

Patent metadata

Field	Value
Publication number	US-10864168-B2
Application number	US-201515521253-A
Country	US
Kind code	B2
Filing date	Oct 28, 2015
Priority date	Oct 29, 2014
Publication date	Dec 15, 2020
Grant date	Dec 15, 2020

Abstract

Provided are lipid membrane microcapsules encapsulating or containing bioactives, and methods of production and use.

First claim

What is claimed is:

1. A method of loading one or more bioactive agents into a lipid membrane microcapsule, comprising:
subjecting the lipid membrane microcapsule in the presence of the one or more bioactive agents to a vacuum pressure of at least about 25% of absolute vacuum levels for a period of 30 minutes or less;
wherein said bioactive agents enter an interior space of said microcapsule.

2. The method of claim 1 , wherein the lipid membrane microcapsule is subjected to the one or more bioactive agents in a solution selected from the group consisting of an aqueous solution and a non-aqueous solution.

3. The method of claim 1 , wherein said lipid membrane microcapsules are sealed in a container where the vacuum pressure (e.g., negative pressure) is in the range of at least about about 50% of absolute vacuum levels to at least about 99% absolute vacuum levels.

4. The method of claim 1 , further comprising, after subjecting the lipid membrane microcapsule to vacuum pressure, subjecting the lipid membrane microcapsule to positive external pressure.

5. The method of claim 1 , wherein the lipid membrane microcapsule is subjected to multiple iterations of vacuum pressure.

6. The method of claim 4 , wherein the lipid membrane microcapsule is subjected to multiple iterations of vacuum pressure and positive external pressure.

7. The method of claim 5 , wherein the bioactive lipid membrane microcapsule is subjected to additional bioactive between each iteration of vacuum pressure.

8. The method of claim 4 , wherein the lipid membrane microcapsule is subjected to positive external pressure for less than 10 minutes.

9. The method of claim 1 , wherein the lipid membrane microcapsule is a cell.

10. The method of claim 9 , wherein the cell comprises a cell wall.

11. The method of claim 10 , wherein the permeability of the cell or cell wall is modified.

12. The method of claim 1 , wherein the lipid membrane microcapsule is a subcellular organelle of a cell or is from a subcellular organelle of a cell.

13. The method of claim 1 , wherein the one or more bioactive agents are selected from the group consisting of a small organic compound, a peptide, a polypeptide, a polynucleotide, and a fatty acid.

14. The method of claim 13 , wherein the one or more bioactive agents have a molecular weight in the range of about 10 Da to about 30 kDa.

15. The method of claim 1 , wherein said one or more bioactive agent is selected from the group consisting of a hydrophobic bioactive, a hydrophilic bioactive, and a combination of at least one hydrophobic bioactive and at least one hydrophilic bioactive agent.

16. The method of claim 4 , wherein said positive external pressure is in the range of about 30 MPa to about 50 MPa.

17. The method of claim 16 , wherein the lipid membrane microcapsule is subjected to a vacuum pressure for less than 30 minutes and a positive external pressure for less than 90 minutes.

18. The method of claim 17 , wherein the lipid membrane microcapsule is subjected to iterations of vacuum pressure and positive external pressure in the range between 2 and 10 iterations.

19. The method of claim 17 , wherein the lipid membrane microcapsule is subjected to iterations of vacuum pressure of between about 3 Torr and about 10 Torr.

Assignees

• Univ California

Inventors

• Nitin Nitin
• Young Stephen
• Vandergheynst Jean

Classifications

• A61K31/352

  condensed with carbocyclic rings, e.g. methantheline  {(cannabinoids A61K31/658)} · CPC title

• A61Q19/00 Primary

  Preparations for care of the skin · CPC title

• A61K8/9728

  Fungi, e.g. yeasts · CPC title

• A23P10/35

  with oils, lipids, monoglycerides or diglycerides · CPC title

• A61K35/745

  Bifidobacteria · CPC title