Method to predict heritable canine non-contact cruciate ligament rupture

Patent metadata

Field	Value
Publication number	US-10858708-B2
Application number	US-201816194659-A
Country	US
Kind code	B2
Filing date	Nov 19, 2018
Priority date	Jan 29, 2015
Publication date	Dec 8, 2020
Grant date	Dec 8, 2020

Abstract

Method and kits for diagnosing propensity to non-contact cranial cruciate ligament rupture (CCLR) in a dog are described. The method includes isolating genomic DNA from a dog and then analyzing the genomic DNA from step for a single nucleotide polymorphism occurring in selected loci that have been determined to be associated with the CCLR phenotype via a genome-wide association study.

First claim

What is claimed is:

1. A method for breeding a dog, the method comprising:
a) isolating genomic DNA from a first dog;
b) assaying the genomic DNA of step (a) for one or more single nucleotide polymorphisms (SNPs) selected from the group consisting of:

Dog

Chromosome

SNP
Location

BICF2G630500368
24

BICF2G630500363
24

BICF2S23152419
27

BICF2S2356299
27

BICF2P483191
29

BICF2P50610
11

BICF2 P890246
9

BICF2823324965
6

BICF2P544126
24

BICF2S24111418
24

BICF2P526639
27

BICF2P1462185
20

BICF2P1208798
9

BICF2G630175389
4

BICF2S24415473
3

BICF2G630412697
30

BICF2P498515
6

TIGRP2P270462
20

BICF2P792911
26

BICF2G630810143
6

BICF2G630810159
6

BICF2P716829
20

BICF2P564273
3

TIGRP2P297337
22

BICF2G630658881
21

BICF2S23255928
27

BICF2P1121006
18

BICF2S23535135
27

BICF2P111342
21
and

BICF2S23346408
28

c) detecting an allele that is not associated with non-contact cranial cruciate ligament rupture at one or more of the SNPs recited in step (b) in the genomic DNA of step (b);
d) selecting a first dog having an allele from step (c) that is not associated with non-contact cranial cruciate ligament rupture; and then
e) breeding the first dog having at least one allele that is not associated with non-contact cranial cruciate ligament rupture at one or more of the SNPs recited in step (b) in the genomic DNA of step (b).

2. The method of claim 1 , wherein step (b) comprises assaying the genomic DNA for five or more single nucleotide polymorphisms (SNPs).

3. The method of claim 1 , wherein step (b) comprises assaying the genomic DNA for ten or more single nucleotide polymorphisms (SNPs).

4. The method of claim 1 , wherein step (b) comprises assaying the genomic DNA for fifteen or more single nucleotide polymorphisms (SNPs).

5. The method of claim 1 , wherein step (b) comprises assaying the genomic DNA for twenty or more single nucleotide polymorphisms (SNPs).

6. The method of claim 1 , wherein step (b) comprises assaying the genomic DNA for SNPs at all of:

Dog

Chromosome

SNP
Location

BICF2G630500368
24

BICF2G630500363
24

BICF2S23152419
27

BICF2S2356299
27

BICF2P483191
29

BICF2P50610
11

BICF2P890246
 9

BICF2S23324965
 6

BICF2P544126
24

Assignees

• Wisconsin Alumni Res Found

Inventors

• Muir Peter
• Baker Lauren

Classifications

• C12Q2600/156

  Polymorphic or mutational markers · CPC title

• C12Q2600/16

  Primer sets for multiplex assays · CPC title

• C12Q2600/118

  Prognosis of disease development · CPC title

• A01K67/02

  Breeding vertebrates · CPC title

• C12Q2600/124

  Animal traits, i.e. production traits, including athletic performance or the like · CPC title