Acidophilic fusarium oxysporum strains, methods of their production and methods of their use

Patent metadata

Field	Value
Publication number	US-10851396-B2
Application number	US-201916442174-A
Country	US
Kind code	B2
Filing date	Jun 14, 2019
Priority date	Jul 3, 2014
Publication date	Dec 1, 2020
Grant date	Dec 1, 2020

Abstract

The present invention provides isolated acidophilic Fusarium oxysporum strains, such as MK7, and their progeny, compositions comprising such strains and their progeny, methods of producing such strains and their progeny, and methods of using such strains and their progeny.

First claim

The invention claimed is:

1. A method of culturing a Fusarium strain, comprising:
(a) culturing an isolated culture of Fusarium MK7 strain, deposited as ATCC Accession Deposit No. PTA-10698, in a liquid culture media comprising a carbon source and having pH of less than 5.0 to produce Fusarium MK7 strain in a form comprising hyphae of Fusarium MK7 strain; and
(b) harvesting the Fusarium MK7 strain.

2. The method of claim 1 , wherein the liquid culture media further comprises an amino acid donor.

3. The method of claim 1 , wherein the step of culturing comprises culturing the isolated culture of Fusarium MK7 strain, deposited as ATCC Accession Deposit No. PTA-10698, in a liquid culture media having pH of less than 4.0.

4. The method of claim 1 , wherein the step of culturing comprises culturing the isolated culture of Fusarium MK7 strain, deposited as ATCC Accession Deposit No. PTA-10698, in a liquid culture media having pH from 0.5 to 3.0.

5. The method of claim 1 , wherein the carbon source is selected from the group consisting of biomass hydrolysates, carbohydrates, cellulose, hemicellulose, xylose, lignin, pectin, monomeric components of the foregoing, and mixtures thereof.

6. The method of claim 1 , wherein the carbon source is selected from the group consisting of a cellulosic biomass hydrolysate.

7. The method of claim 1 , wherein the step of culturing comprises a batch fermentation process.

8. The method of claim 7 , wherein the batch fermentation process comprises inoculating a fermenter comprising substrate with the Fusarium MK7 strain, deposited as ATCC Accession Deposit No. PTA-10698.

9. The method of claim 7 , wherein the liquid culture media in the batch fermentation process is under microaerobic conditions.

10. The method of claim 1 , further comprising a pretreatment step prior to step (a), wherein the pretreatment step is selected from the group consisting of: reducing the pH of the liquid culture media, adding manganese to the liquid culture media, adding a nutrient to the liquid culture media, and combinations thereof.

11. The method according to claim 10 , wherein, after the pretreatment step, the pH of the liquid culture media is from about 0.5 to about 3.0 and the manganese concentration in the liquid culture media is 5 mM, 10 mM, or 25 mM.

Assignees

• Sustainable Bioproducts Inc
• The Fynder Group Inc

Inventors

• Kozubal Mark A
• Macur Richard E
• Inskeep William P

Classifications

• C12R2001/77

  Fusarium · CPC title

• C12N1/145

  Fungi isolates · CPC title

• C12P7/6463

  obtained from glyceride producing microorganisms, e.g. single cell oil · CPC title

• C12P7/10

  substrate containing cellulosic material · CPC title

• C12P7/065

  with microorganisms other than yeasts · CPC title