Decanedioic acid produced by microbial fermentation process and preparation method thereof

Patent metadata

Field	Value
Publication number	US-10851394-B2
Application number	US-201816049119-A
Country	US
Kind code	B2
Filing date	Jul 30, 2018
Priority date	Aug 2, 2017
Publication date	Dec 1, 2020
Grant date	Dec 1, 2020

Abstract

The present invention provides a decanedioic acid produced by microbial fermentation process, in which the content of C10 aliphatic acid and C10 hydroxy aliphatic acid is maintained at a very low level. The present invention also provides a preparation method of the decanedioic acid and a polymer prepared by using the decanedioic acid as monomer. The decanedioic acid provided by the present invention is prepared by microbial fermentation process. The decanedioic acid product which is produced through the processes of microbial fermentation and separation has a higher purity, a higher thermal stability, and a lower impurity content. The decanedioic acid provided by the present invention could satisfy the requirements of high grade product of polyamide or polyester to produce polymer with excellent qualities. The preparation method of the decanedioic acid provided by the present invention which has many advantages of mild reaction conditions, environmental friendliness, high yield, and good product quality, may replace the chemical method to be used in industrial scale production of the decanedioic acid.

First claim

What is claimed is:

1. A method of preparing decanedioic acid, comprising:
culturing Candida tropicalis to obtain a seed culture;
inoculating the obtained seed culture with a fermentation medium;
starting a fermentation process to produce fermentation broth and adding decane when OD 620 of the fermentation medium with a dilution of 1:30 reaches more than 0.5; and
purifying the obtained fermentation broth to yield decanedioic acid.

2. The method according to claim 1 , wherein the fermentation medium comprises: 0-50 g/L glucose, 1-10 g/L potassium nitrate, 2-10 g/L corn steep liquor, 1-8 g/L yeast extract, 1-10 g/L potassium phosphate monobasic, and 1-4 g/L ammonium sulfate.

3. The method according to claim 1 , wherein the OD 620 of the fermentation medium is maintained at 0.3-0.8 in the fermentation process.

4. The method according to claim 1 , wherein relative dissolved oxygen is maintained at more than 10% (v/v) in the fermentation process.

5. The method according to claim 1 , wherein the pH value is maintained at 5.0-8.0 in the fermentation process.

6. The method according to claim 1 , wherein the content of decane in fermentation system is maintained at more than or equal to 1% (v/v) by feeding the decane in a continuous way or in a batch way in the fermentation process, and wherein the content of decane in the obtained fermentation broth is maintained at less than or equal to 0.5% (v/v) when the fermentation process is terminated.

7. The method according to claim 1 , wherein the Candida tropicalis is Candida tropicalis CAT N145.

8. The method according to claim 1 , wherein the purifying the obtained fermentation broth comprises:
removing impurities by one or more separation processes consisting of membrane filtration, plate and frame filtration, and centrifugation to obtain decanedioic acid crude product;
purifying the obtained decanedioic acid crude product by one or more crystallization processes to obtain decanedioic acid crystals; and
washing, and drying decanedioic acid crystals to yield decanedioic acid.

9. The method according to claim 8 , wherein a crystallization solvent is used in the one or more crystallization processes in the step of purifying the obtained decanedioic acid crude product.

10. The method according to claim 9 , wherein the crystallization solvent is selected from the group consisting of water, ethanol, acetic acid, ethyl acetate, and butyl acetate.

11. The method according to claim 10 , wherein the crystallization solvent is acetic acid and the content of acetic acid in the obtained decanedioic acid crystals is maintained at less than 500 ppm.

12. The method according to claim 1 , wherein relative dissolved oxygen is maintained at 10% to 70% in the fermentation process.

13. The method according to claim 11 , wherein the content of acetic acid in the obtained decanedioic acid crystals is maintained at less than 300 ppm.

14. A decanedioic acid product produced by the method according to claim 1 .

15. The decanedioic acid product according to claim 14 , wherein the content of aliphatic acid comprising 10 carbons is less than 200 ppm.

16. The decanedioic acid product according to claim 14 , wherein the thermal stability of decanedioic acid product is more than 90%.

Assignees

• Cathay Biotech Inc
• Cibt America Inc

Inventors

• Xu Min
• Hao Yingli
• Yang Chen
• Li Naiqiang
• Liu Xiucai

Classifications

• C12P7/44 Primary

  Polycarboxylic acids · CPC title

• C08G63/16

  Dicarboxylic acids and dihydroxy compounds · CPC title

• C07C55/20

  Sebacic acid · CPC title

• C08G63/12

  derived from polycarboxylic acids and polyhydroxy compounds · CPC title

• C08G69/26

  derived from polyamines and polycarboxylic acids · CPC title