Flow assembly for cells

Patent metadata

Field	Value
Publication number	US-10851335-B2
Application number	US-201615564139-A
Country	US
Kind code	B2
Filing date	Mar 31, 2016
Priority date	Apr 8, 2015
Publication date	Dec 1, 2020
Grant date	Dec 1, 2020

Abstract

In one embodiment, a flow assembly for cells comprises a first flow path configured to receive a plurality of cells, a second flow path configured to receive a buffer, and a third flow path configured to receive the plurality of cells and the buffer. The plurality of cells are in a single-file orientation and the buffer generally surrounds the single-file orientation of the plurality of cells when in the third flow path.

First claim

What is claimed is:

1. A flow assembly for cells, comprising:
a joining member, the joining member including:
a cell flow path configured to receive a plurality of cells and having an inner diameter of 50-100 μm; and
a single buffer flow path configured to receive a buffer and having an inner diameter of 100-200 μm; and

a third flow path configured to receive the plurality of cells and the buffer and having an inner diameter greater than the inner diameters of the cell flow path and the single buffer flow path throughout an entire length of the third flow path, the plurality of cells being in a single-file orientation with the buffer surrounding the single-file orientation of the plurality of cells when in the third flow path, such that the buffer forms a chamber through which the plurality of cells flow through a middle of the chamber in the single-file orientation.

2. The flow assembly of claim 1 , wherein the inner diameter of the cell flow path is 75 μm.

3. The flow assembly of claim 1 , wherein the inner diameter of the single buffer flow path is 150 μm.

4. The flow assembly of claim 1 , wherein the inner diameter of the third flow path is 0.3-2.0 mm.

5. The flow assembly of claim 1 , wherein the buffer is phosphate buffered saline.

Assignees

• Univ Indiana Res & Tech Corp

Inventors

• Jones Lisa M
• Rinas Aimee

Classifications

• C12M29/00 Primary

  Means for introduction, extraction or recirculation of materials, e.g. pumps (pumps per se F04B) · CPC title

• C12M33/00

  Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus (chemical or physical laboratory apparatus in general B01L, devices for taking cell samples A61B10/0045, withdrawing or distributing predetermined quantities of fluid B01L99/00) · CPC title

• C12M23/16 Primary

  Microfluidic devices; Capillary tubes (integrated microfluidic structures B01L3/5027; microreactors B01J19/0093) · CPC title

• C12M41/46

  of cellular or enzymatic activity or functionality, e.g. cell viability · CPC title

• C12M1/34

  Measuring or testing with condition measuring or sensing means, e.g. colony counters · CPC title