Electrochemical approach for COVID-19 detection

Patent metadata

Field	Value
Publication number	US-10845336-B2
Application number	US-202016924718-A
Country	US
Kind code	B2
Filing date	Jul 9, 2020
Priority date	Apr 21, 2020
Publication date	Nov 24, 2020
Grant date	Nov 24, 2020

Abstract

A method for diagnosing COVID-19 infection of a person. The method includes acquiring a sputum sample of a person, measuring a level of ROS in the sputum sample, and detecting a COVID-19 infection status of the person based on the measured level of ROS. Measuring the level of ROS in the sputum sample includes recording a cyclic voltammetry (CV) pattern from the sputum sample and measuring a current peak of the recorded CV pattern. Detecting the COVID-19 infection status of the person includes detecting COVID-19 infection of the person responsive to the measured current peak being in a first range of more than 230 μA and detecting COVID-19 non-infection of the person responsive to the measured current peak being in a second range of less than 190 μA.

First claim

What is claimed is:

1. A method for diagnosing COVID-19 infection of a person, comprising:
acquiring a sputum sample of the person;
measuring a level of reactive oxygen species (ROS) in the sputum sample, comprising:
recording a cyclic voltammetry (CV) pattern from the sputum sample; and
measuring a current peak of the recorded CV pattern; and

detecting a COVID-19 infection status of the person based on the measured level of ROS, comprising:
detecting that the person is infected with COVID-19 if the measured current peak is in a first range of current peaks being more than 230 μA; and
detecting that the person is not infected with COVID-19 if the measured current peak is in a second range of current peaks being less than 190 μA.

2. The method of claim 1 , wherein detecting the COVID-19 infection status of the person further comprises detecting a suspicious condition of COVID-19 infection of the person if the measured current peak is in a third range of current peaks between 190 μA and 230 μA,
wherein the suspicious condition of COVID-19 infection of the person comprises an indication that there is no definite determination regarding COVID-19 infection of the person.

3. The method of claim 1 , further comprising generating a calibration data set, comprising:
generating the first range of current peaks, comprising:
recording a first set of CV patterns from a plurality of persons infected with COVID-19 virus; and
measuring a first set of current peaks of the first set of CV patterns; and

generating the second range of current peaks, comprising:
recording a second set of CV patterns from a plurality of persons not infected with COVID-19 virus; and
measuring a second set of current peaks of the second set of CV patterns.

4. The method of claim 3 , wherein detecting the COVID-19 infection status of the person further comprises comparing the measured current peak with current peak values in the calibration data set.

5. The method of claim 1 , wherein recording the CV pattern from the sputum sample comprises:
attaching a sensing head of an electrochemical probe to a handle of the electrochemical probe, the sensing head comprising three needle-shaped electrodes located at one end of the sensing head;
connecting the handle of the electrochemical probe to an electrochemical stimulator-analyzer device;
inserting the three needle-shaped electrodes into the sputum sample;
applying a sweeping range of electrical potential from −0.8 V to +0.8 V with a scan rate of 100 mVs −1 to the electrochemical probe utilizing the electrochemical stimulator-analyzer device; and
recording the CV pattern comprising a set of electrical currents versus the applied sweeping range of electrical potential.

6. The method of claim 5 , wherein each of the three needle-shaped electrodes comprises:
a steel needle comprising a sharp tip; and
an array of multi-walled carbon nanotubes (MWCNTs) grown on the sharp tip, wherein the three needle-shaped electrodes are attached at the one end of the sensing head with a distance between 1 mm and 5 mm between each two respective needle-shaped electrodes placed next to each other.

7. The method of claim 1 , wherein measuring the level of ROS in the sputum sample comprises measuring the level of mitochondrial ROS induced by COVID-19 virus in respiratory epithelial host cells of the person.

8. The method of claim 1 , wherein acquiring the sputum sample of the person comprises:
allowing the person to enter a sampling cabin, the sampling cabin configured to:
isolate the person in a closed area, and
apply a set of controlled conditions while acquiring the sputum sample;

putting a fresh sputum into a sampling container by the person; and
handing over the fresh sputum to an individual in a testing room located next to the sampling cabin, comprising transferring the fresh sputum from the sampling cabin to the testing room by the person utilizing an interface drawer, the interface drawer being movable between the sampling cabin and the testing room.

9. The method of claim 8 , wherein applying the set of controlled conditions to the sampling cabin comprises:
spraying a disinfectant agent into the sampling cabin; and
displacing air through the sampling cabin, comprising:
flowing air inside the sampling cabin from a bottom side of the sampling cabin using a high-pressure air blower fan; and
flowing air outside the sampling cabin using an air suction fan placed above the sampling cabin.

10. The method of claim 1 , wherein acquiring the sputum sample of the person comprises taking a bronchoalveolar lavage (BAL) fluid from a hospitalized patient.

Assignees

• Abdolahad Mohammad
• Sadat Miripour Zohreh
• Sanati Koloukhi Hassan
• Shojaeian Zanjani Fatemeh Zahra

Inventors

• Abdolahad Mohammad
• Sadat Miripour Zohreh
• Sanati Koloukhi Hassan
• Shojaeian Zanjani Fatemeh Zahra

Classifications

• G01N27/3275

  Sensing specific biomolecules, e.g. nucleic acid strands, based on an electrode surface reaction · CPC title

• G01N2800/26

  Infectious diseases, e.g. generalised sepsis · CPC title

• G01N33/48771

  Coding of information, e.g. calibration data, lot number · CPC title

• G01N27/48 Primary

  using polarography, i.e. measuring changes in current under a slowly-varying voltage · CPC title

• G01N33/48714 Primary

  for determining substances foreign to the organism, e.g. drugs or heavy metals (drugs by chemical analysis G01N33/94) · CPC title