Quantification of topologically arranged luminescent dyes

Patent metadata

Field	Value
Publication number	US-10845310-B2
Application number	US-201615737747-A
Country	US
Kind code	B2
Filing date	Jun 29, 2016
Priority date	Jun 30, 2015
Publication date	Nov 24, 2020
Grant date	Nov 24, 2020

Abstract

A sensor device for quantifying luminescent targets configured in an at least one dimensional pattern. The sensor device comprises a detector for obtaining an at least one dimensional pattern of measured signals, wherein the detector is adapted for detecting the luminescence of the luminescent targets, resulting in a measured pattern. The sensor device moreover comprises a processor configured to correlate the measured pattern with at least one reference pattern, so as to generate a measurement signal representative for the quantification of luminescent targets. The at least one reference pattern is a recorded pattern or an expected pattern. A recorded pattern is a pattern which is obtained by the detector before the measured pattern is obtained.

First claim

The invention claimed is:

1. A sensor device for quantifying luminescent targets configured in an at least one dimensional pattern, the sensor device comprising:
a detector for obtaining an at least one-dimensional pattern of measured signals, wherein the detector is adapted for detecting a luminescence of the luminescent targets, resulting in a measured pattern;
a processor configured to correlate the measured pattern with at least one reference pattern so as to generate a measurement signal representative for quantification of the luminescent targets, wherein the at least one reference pattern is an expected pattern or a recorded pattern obtained by the detector before the measured pattern is obtained;
a light source for exciting the luminescent targets, thus generating luminescence signals; and
a bleaching device comprising a second light source adapted for generating a high intensity pulse for bleaching of at least part of sources generating a background signal,
wherein a desired signal is part of the pattern of measured signals that is originating from the luminescent targets,
wherein the background signal is different from the desired signal in the pattern of measured signals,
wherein the processor is configured to trigger the bleaching device to start bleaching at a moment in time after substantial saturation of the background signal and before saturation of the desired signal and to trigger the light source for exciting remaining luminescent targets, and
wherein the remaining luminescent targets are those targets which are not bleached, and to trigger the detector for detecting the luminescence signal of the remaining luminescent targets, so as to generate a measurement signal representative for quantification of the luminescent targets.

2. The sensor device according to claim 1 , further comprising a surface for binding the luminescent targets, wherein the surface is designed such that the luminescent targets bind on a location of the surface corresponding with an at least one-dimensional binding pattern, and wherein the at least one reference pattern is the at least one-dimensional binding pattern of the surface.

3. The sensor device according to claim 1 , further comprising:
a surface or a three dimensional volume for binding the luminescent targets;
an evanescent field generating structure, wherein a light source is coupled to the evanescent field generating structure, and wherein the evanescent field generating structure is adapted for generating an evanescent field at the surface or in the three dimensional volume.

4. The sensor device according to claim 1 , wherein the processor is configured to correlate subsections of the measured pattern with subsections of the reference pattern.

5. The sensor device according to claim 1 ,
wherein the sensor device comprises a modulator which is configured to modulate a physical parameter, resulting in modulation of a desired signal that is different from the modulation of a background signal,
wherein the desired signal is this part of the pattern of measured signals that is originating from the luminescent targets, and
wherein the background signal is different from the desired signal in the pattern of measured signals, wherein the processor is configured to correlate, while taking into account the modulation of the physical parameter, the measured pattern with the at least one reference pattern recorded at a different time instance than the measured pattern.

6. The sensor device according to claim 1 , further comprising a light source configured to excite the luminescent targets at two different wavelengths, wherein the recorded pattern is obtained when exciting at one wavelength, and wherein the measured pattern is obtained when exciting at another wavelength.

7. A diagnostic device comprising
the sensor device according to claim 1 for sensing an analyte and generating a measurement signal representative for quantification of the analyte; and
an output unit for providing an output of said sensor device on which a diagnosis can be based.

8. The diagnostic device according to claim 7 , wherein the output unit is adapted for outputting a signal representative for presence/absence or concentration of the analyte.

Assignees

• Imec Vzw

Inventors

• Peumans Peter
• Lagae Liesbet
• Van Roy Willem
• Stakenborg Tim
• Van Dorpe Pol

Classifications

• G01N2021/6432

  Quenching · CPC title

• G01N2021/6421

  Measuring at two or more wavelengths · CPC title

• G01N21/6452 Primary

  Individual samples arranged in a regular 2D-array, e.g. multiwell plates · CPC title

• G01N21/648

  using evanescent coupling or surface plasmon coupling for the excitation of fluorescence · CPC title

• G01N21/1717

  with a modulation of one or more physical properties of the sample during the optical investigation, e.g. electro-reflectance · CPC title