Filter systems for separating microcarriers from cell culture solutions
US-10301585-B2 · May 28, 2019 · US
US10843141B2 · US · B2
| Field | Value |
|---|---|
| Publication number | US-10843141-B2 |
| Application number | US-201916510438-A |
| Country | US |
| Kind code | B2 |
| Filing date | Jul 12, 2019 |
| Priority date | Sep 30, 2011 |
| Publication date | Nov 24, 2020 |
| Grant date | Nov 24, 2020 |
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A container assembly includes a flexible bag having an interior surface bounding a chamber and an opposing exterior surface. The bag has a bottom end wall that includes a first sparger and a second sparger. The first sparger and the second sparger each have a flexible first sheet overlying a flexible second sheet, the first sheet and the second sheet being secured together so as to form a sparging area bounded between the first sheet and the second sheet, at least a portion of the first sheet overlying the sparging area being gas permeable so that gas can pass from the sparging area, through the first sheet, and into the chamber.
Opening claim text (preview).
What is claimed is: 1. A container assembly comprising a flexible bag having an interior surface bounding a chamber and an opposing exterior surface, the bag having a bottom end wall being comprised of a first sparger and a second sparger, the first sparger and the second sparger each comprising a flexible first sheet overlying a flexible second sheet, the first sheet and the second sheet being secured together so as to form a sparging area bounded between the first sheet and the second sheet, at least a portion of the first sheet overlying the sparging area being gas permeable so that gas can pass from the sparging area, through the first sheet, and into the chamber. 2. The container assembly as recited in claim 1 , wherein the first sheet of the first sparger and the first sheet of the second sparger comprise a single, continuous sheet. 3. The container assembly as recited in claim 1 , wherein the second sheet of the first sparger and the second sheet of the second sparger comprise a single, continuous sheet. 4. The container assembly as recited in claim 1 , wherein the first sheet and the second sheet of the first sparger and the first sheet and the second sheet of the second sparger are each comprised of a polymeric film. 5. The container assembly as recited in claim 1 , further comprising a first gas line coupled with the sparging area of the first sparger and a second gas line coupled with the sparging area of the second sparger. 6. The container assembly as recited in claim 5 , wherein the first gas line and the second gas line are each secured to a manifold. 7. The container assembly as recited in claim 6 , wherein the manifold comprises: a first valve that regulates the flow of gas through the manifold and to the first sparger; and a second valve that regulates the flow of gas through the manifold and to the second sparger. 8. The container assembly as recited in claim 1 , further comprising: a first port secured to the first sheet or the second sheet of the first sparger; and a first gas line coupled with the first port. 9. The container assembly as recited in claim 1 , further comprising the bag having a top end wall, the bottom end wall, and an encircling sidewall extending therebetween, the first sheet or the second sheet of the first sparger being connected directly to or being integrally formed as a single continuous member with at least a portion of the encircling sidewall. 10. The container assembly as recited in claim 9 , wherein both the first sheet and the second sheet of the first sparger extend to the encircling sidewall. 11. The container assembly as recited in claim 1 , wherein the first sheet and the second sheet of the first sparger are secured together by welding. 12. The container assembly as recited in claim 1 , wherein the first sparger is separated from the second sparger. 13. The container assembly as recited in claim 1 , wherein the first sparger is isolated from the second sparger so that a gas entering the first sparger does not enter the second sparger. 14. The container assembly as recited in claim 1 , further comprising first perforations extending through the first sheet of the first sparger and second perforations extending through the first sheet of the second sparger, the first perforations having a different size than the second perforations. 15. The container assembly as recited in claim 1 , further comprising first perforations extending through the first sheet of the first sparger and second perforations extending through the first sheet of the first sparger, the first perforations having a different size than the second perforations. 16. The container assembly as recited in claim 1 , wherein the first sparger has a different configuration than the second sparger. 17. The container assembly as recited in claim 1 , further comprising a rigid support housing bounding a compartment, the flexible bag being disposed within the compartment of the support housing. 18. The container assembly as recited in claim 1 , wherein the bottom end wall further comprises a third sparger, the third sparger comprising a flexible first sheet overlying a flexible second sheet, the first sheet and the second sheet of the third sparger being secured together so as to form a sparging area bounded between the first sheet and the second sheet, at least a portion of the first sheet of the third sparger being gas permeable.
Bags · CPC title
Flexible receptacles, e.g. bags supported by rigid containers · CPC title
being spirally wound, coiled tubes or spirally wound, coiled and joined bands or wires · CPC title
being in the form of rings or annular elements · CPC title
having disc shape · CPC title
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