Composition of matter comprising liposomes embedded in a polymeric matrix and methods of using same

Patent metadata

Field	Value
Publication number	US-10842745-B2
Application number	US-201916254084-A
Country	US
Kind code	B2
Filing date	Jan 22, 2019
Priority date	Oct 7, 2008
Publication date	Nov 24, 2020
Grant date	Nov 24, 2020

Abstract

The present disclosure provides a composition of matter comprising liposomes encapsulating in their intraliposomal aqueous compartment at least one active agent, the liposomes having a diameter of at least 200 nm and being embedded in a water insoluble, water absorbed cross-linked polymeric matrix. In one embodiment, the composition of matter is held within an aqueous medium, preferably being in iso-osmotic equilibrium with the intraliposomal aqueous compartments of the liposomes. The present disclosure also provides a method of removal of non-encapsulated active agent from the composition of matter, a method of preparing said composition of matter, a pharmaceutical composition comprising said composition of matter, use of such composition of matter; a method of providing prolonged delivery of a active agent to a subject in need thereof by administering to said subject the composition of matter disclosed herein as well as a package comprising said composition of matter held within said aqueous medium and instructions for use thereof.

First claim

The invention claimed is:

1. A composition comprising:
(a) an external storage solution having a first osmolality comprising at least one water soluble lipid permeable active agent having a first concentration, wherein the first water soluble lipid permeable active agent has a molecular weight of less than or equal to 500 Da, and a log P of greater than 2.2; and
(b) particles contained within the external storage solution wherein the particles comprise (i) a water insoluble water absorbed cross-linked polymeric matrix and (ii) liposomes contained within the water insoluble water absorbed cross-linked polymeric matrix,
wherein the lipid comprises cholesterol and a phosphatidylcholine lipid having a Tm greater than or equal to 41° C.;
wherein the liposomes have an intraliposomal aqueous compartment containing an intraliposomal solution having a second osmolality, comprising the at least one water soluble, lipid permeable active agent having a molecular weight of less than or equal to 500 Da, and a log P of greater than 2.2 at a second concentration;
wherein the first osmolality is not more than 50 mOsmoles higher than the second osmolality; and
wherein the second concentration is at least three times greater than the first concentration.

2. The composition of claim 1 wherein the active agent is bupivacaine.

3. The composition of claim 1 wherein the first concentration increases not more than 2-fold during storage at 4° C. for a period of at least three months.

4. The composition of claim 1 wherein the first concentration increases not more than 2-fold during storage at 4° C. for a period of at least four and a half months.

5. The composition of claim 1 wherein the first concentration increases not more than 2-fold during storage at 4° C. for a period of at least six months.

6. The composition of claim 1 wherein the first concentration increases not more than 2-fold during storage at 15° C. for a period of at least three months.

7. The composition of claim 1 wherein the first concentration increases not more than 2-fold during storage at 22° C. for a period of at least three months.

8. The composition of claim 1 wherein the first concentration increases not more than 2-fold during storage at 37° C. for a period of at least three months.

9. The composition of claim 1 wherein the liposomes are at least one of multilamellar vesicles (MLVs) or multivesicular vesicles (MVVs).

10. The composition of claim 1 wherein the polymer of the matrix is a fully cross-linked polymer or a partially cross-linked polymer.

11. The composition of claim 10 wherein the polymer matrix is a hydrogel comprising at least one cross-linked polysaccharide, the polysaccharide selected from the group consisting of alginate and chitosan.

12. The composition of claim 1 wherein the first concentration of the at least one water soluble, lipid permeable active agent is between 0.2% and 2.0%.

13. The composition of claim 1 wherein the liposomes are large multivesicular vesicles (LMVVs) having a diameter of between 200 nm and 25 μm.

14. The composition of claim 1 wherein the particles are administered to a subject in need thereof.

15. A method for providing analgesia in a patient in pain by administering the composition of claim 2 to the patient and wherein the external storage solution is removed from the particles prior to administering the particles to the patient.

16. The method of claim 15 wherein the administration of the particles is a local administration.

17. A composition comprising:
(a) an external storage solution having a first osmolality comprising at least one water soluble lipid permeable active agent having a first concentration, wherein the first water soluble lipid permeable active agent has a molecular weight of less than or equal to 500 Da, and a log P of greater than 2.2; and
(b) particles contained within the external storage solution wherein the particles comprise (i) a water insoluble water absorbed cross-linked polymeric matrix and (ii) liposomes contained within the water insoluble water absorbed cross-linked polymeric matrix, wherein the lipid comprises cholesterol and a phosphatidylcholine lipid having a Tm greater than or equal to 41° C.;
wherein the liposomes are large multivesicular vesicles (LMVVs) having a diameter of between 200 nm and 25 μm;
wherein the liposomes have an intraliposomal aqueous compartment containing an intraliposomal solution having a second osmolality, comprising the at least one water soluble, lipid permeable active agent having a molecular weight of less than or equal to 500 Da, and a log P of greater than 2.2 at a second concentration;
wherein the first osmolality is not more than 50 mOsmoles higher than the second osmolality; wherein the second concentration is at least three times greater than the first concentration; and
wherein the first concentration increases not more than 2-fold during storage at 4° C. for a period of at least three months.

18. A method for providing analgesia in a patient in pain by administering the composition of claim 17 to the patient, wherein the composition comprises a lipid permeable active agent, wherein the analgesic active agent is bupivacaine, and wherein the external storage solution is removed from the particles prior to administering the particles to the patient.

19. The method of claim 18 wherein the administration of the particles is a local administration.

Assignees

• Yissum Res Dev Co Of Hebrew Univ Jerusalem Ltd

Inventors

• Barenholz Yechezkel
• Cohen Rivka

Classifications

• A61K31/445

  Non condensed piperidines, e.g. piperocaine · CPC title

• A61K9/127 Primary

  Synthetic bilayered vehicles, e.g. liposomes or liposomes with cholesterol as the only non-phosphatidyl surfactant · CPC title

• A61P29/00

  Non-central analgesic, antipyretic or antiinflammatory agents, e.g. antirheumatic agents; Non-steroidal antiinflammatory drugs [NSAID] · CPC title

• A61K31/573

  substituted in position 21, e.g. cortisone, dexamethasone, prednisone or aldosterone · CPC title

• A61P23/02

  Local anaesthetics · CPC title