Method for detecting notoginseng using terahertz technology

Patent metadata

Field	Value
Publication number	US-10837900-B2
Application number	US-201916388480-A
Country	US
Kind code	B2
Filing date	Apr 18, 2019
Priority date	Apr 18, 2018
Publication date	Nov 17, 2020
Grant date	Nov 17, 2020

Abstract

A method for detecting notoginseng using terahertz technology. The notoginseng samples are pulverized in pulverizer. The pulverized samples are mixed with polyethylene powder in the ratio of 1:4 to 1:6. The powder mixture is pressed in a tablet press to obtain notoginseng tablet. Terahertz spectrograph is started. A peak is saved, and a background signal is measured. The time-domain graph of the background without sample is acquired. The time-domain graphs of the notoginseng tablets are acquired. The time-domain graphs are transformed into frequency-domain graphs by Fourier transform. The frequency-domain graphs are converted to obtain absorption spectrum of the notoginseng tablets. The absorption peak position of test tablet is compared with that of standard tablet to determine its authenticity. The concentration of key active substances of the notoginseng test sample is quantitatively analyzed by integrating the area under absorption peak curve of the notoginseng test sample.

First claim

What is claimed is:

1. A method for detecting notoginseng using terahertz technology, comprising:
1) pulverizing a notoginseng standard sample and a notoginseng test sample separately in a pulverizer; then mixing the pulverized standard sample and the pulverized test sample respectively with a polyethylene powder with the same ratio of between 1:4 and 1:6 to obtain a standard powder mixture and a test powder mixture;
2) pressing the standard powder mixture and the test powder mixture separately in a tablet press to obtain a standard tablet and a test tablet for detection;
3) reducing humidity in a sample chamber of a terahertz spectrograph to less than 3%; storing a peak position; measuring a single channel of background; and acquiring a time-domain graph of the background without sample;
4) placing the standard tablet and the test tablet on a sample holder successively;
acquiring a time-domain graph of the standard tablet and a time-domain graph of the test tablet, respectively;
5) transforming the time-domain graphs of the background, the standard tablet and the test tablet into frequency-domain graphs by Fourier transform;
6) converting the frequency-domain graphs of the standard tablet, the test tablet and the background to obtain absorption spectra of the standard tablet and the test tablet by the following equation:

Ab
=

-

log
⁡

(

Sam
Ref

)

;

where, Ab is the absorption spectrum data, Sam is the standard frequency-domain data, and Ref is the reference frequency-domain data;
7) repeating test to obtain multiple sets of absorption spectrum data of the test tablet; averaging the obtained multiple sets of the absorption spectrum data; comparing an absorption spectrum of the test tablet obtained by analysis software according to the averaged absorption spectrum data with that of the standard tablet, wherein a comparison between absorption peak positions of the test tablet and absorption peak position of the standard tablet are made to determine authenticity of the notoginseng test sample; wherein, when position errors of absorption peak of the test notoginseng tablet are within a resolution range of the terahertz spectrograph, the notoginseng sample is authentic, otherwise it is fake notoginseng;
8) when it is determined that the test sample is authentic, quantitatively analyzing a concentration of key active substances of the test tablet by integrating an area under an absorption peak curve of the tablet notoginseng test sample; wherein the area under the absorption peak curve of the test tablet is calculated, and then compared with an area under an absorption peak curve of the standard tablet whose concentration of the key active substances is known to obtain an area ratio; the concentration of key active substances of the notoginseng test sample is calculated according to the area ratio with the same amount of samples.

2. The method of claim 1 , wherein in step 7), whether the position errors of absorption peak are within the resolution range of the terahertz spectrograph is further determined.

Assignees

• Univ Of Shanghai For Science And Technology

Inventors

• Peng Yan
• Zhu Yiming
• Kou tianyi
• Sun Zhaozhao
• Xiao Haicheng
• Shi Chenjun
• Wang Liping
• Zhuang Songlin

Classifications

• G01N21/3586 Primary

  by Terahertz time domain spectroscopy [THz-TDS] · CPC title

• G01N21/01

  Arrangements or apparatus for facilitating the optical investigation · CPC title

• G01N2021/3572

  Preparation of samples, e.g. salt matrices · CPC title

• G01N21/3563 Primary

  for analysing solids; Preparation of samples therefor · CPC title