Methods and compositions for producing corn plants with resistance to late wilt

Patent metadata

Field	Value
Publication number	US-10837067-B2
Application number	US-201715674917-A
Country	US
Kind code	B2
Filing date	Aug 11, 2017
Priority date	Aug 11, 2016
Publication date	Nov 17, 2020
Grant date	Nov 17, 2020

Abstract

The present disclosure is in the field of plant breeding and disease resistance. The disclosure provides methods for breeding corn plants having late wilt (LW) resistance using marker-assisted selection. The disclosure further provides corn germplasm resistant to LW. The disclosure also provides markers associated with LW resistance loci for introgressing these loci into elite germplasm in a breeding program, thus producing novel LW resistant germplasm.

First claim

What is claimed is:

1. A method of creating a population of Late Wilt (LW) resistant corn plants or corn seeds, said method comprising:
a) obtaining a first progeny population of plants seeds;
b) genotyping said first progeny population of corn seeds or plants grown therefrom at one or more marker loci linked to, and within 10 centimorgans (cM) of LW resistance quantitative trait locus (QTL) LW_2.01;
c) selecting from said first progeny population one or more corn plants or corn seeds comprising said one or more of said marker loci linked to said LW resistant QTL LW_2.01 linked to an LW resistance allele selected from the group consisting of:
SEQ ID NO: 8, comprising a T at position 244;
SEQ ID NO: 9, comprising a G at position 277;
SEQ ID NO: 10, comprising an A at position 430;
SEQ ID NO: 11, comprising a C at position 269;
SEQ ID NO: 12, comprising a G at position 61;
SEQ ID NO: 13, comprising an A at position 61;
SEQ ID NO: 14, comprising a T at position 61;
SEQ ID NO: 15, comprising a T at position 138;
SEQ ID NO: 16, comprising a C at position 61;
SEQ ID NO: 17, comprising an A at position 101;
SEQ ID NO: 21, comprising a C at position 148;
SEQ ID NO: 22, comprising a C at position 101;
SEQ ID NO: 23, comprising a C at position 101;
SEQ ID NO: 26, comprising an A at position 674;
SEQ ID NO: 27, comprising a G at position 298;
SEQ ID NO: 28, comprising a G at position 483;
SEQ ID NO: 29, comprising an A at position 44;
SEQ ID NO: 30, comprising a C at position 101;
SEQ ID NO: 31, comprising a T at position 90;
SEQ ID NO: 32, comprising a G at position 871;
SEQ ID NO: 33, comprising a G at position 460;
SEQ ID NO: 34, comprising an A at position 252
SEQ ID NO: 35, comprising an A at position 225;
SEQ ID NO: 36, comprising a T at position 232;
SEQ ID NO: 37, comprising an A at position 321; and
SEQ ID NO: 38, comprising a G at position 101; and

d) crossing said selected one or more corn plants or corn plants grown from said seeds with a second corn plant to produce a second progeny corn seed or plant grown therefrom comprising said one or more of said marker loci linked to said LW resistance allele at said LW resistant QTL LW_2.01.

2. The method of claim 1 , further comprising: crossing a first LW resistant corn plant comprising said LW resistance QTL LW_2.01 with a second corn plant of a different genotype to produce said first progeny population of corn plants or corn seeds.

3. The method of claim 1 , wherein said LW resistance QTL LW_2.01 provides mild resistance or resistance to infection by Harpophora maydis.

4. The method of claim 1 , wherein said second progeny corn plants exhibits reduced dark yellow to brownish macerated pith, brownish-black vascular bundle, or both compared to corn plants or corn seeds lacking said LW resistance QTL LW_2.01 under a similar growth condition.

5. The method of claim 1 , wherein said step b) comprises assaying a single nucleotide polymorphism (SNP) marker.

6. The method of claim 1 , wherein said step b) comprises the use of an oligonucleotide probe.

7. The method of claim 6 , wherein said oligonucleotide probe is adjacent to a polymorphic nucleotide position in said marker locus.

8. The method of claim 1 , wherein said step b) comprises detecting a haplotype.

9. A method of creating a population of Late Wilt (LW) resistant corn plants or corn seeds, said method comprising:
a) genotyping a first population of corn plants or corn seeds at one or more marker loci linked to, and within 10 centimorgans (cM) of any one of marker loci linked to an LW resistant allele selected from the group consisting of:
SEQ ID NO: 8, comprising a T at position 244;
SEQ ID NO: 9, comprising a G at position 277;
SEQ ID NO: 10, comprising an A at position 430;
SEQ ID NO: 11, comprising a C at position 269;
SEQ ID NO: 12, comprising a G at position 61;
SEQ ID NO: 13, comprising an A at position 61;
SEQ ID NO: 14, comprising a T at position 61;
SEQ ID NO: 15, comprising a T at position 138;
SEQ ID NO: 16, comprising a C at position 61;
SEQ ID NO: 17, comprising an A at position 101;
SEQ ID NO: 21, comprising a C at position 148;
SEQ ID NO: 22, comprising a C at position 101;
SEQ ID NO: 23, comprising a C at position 101;
SEQ ID NO: 26, comprising an A at position 674;
SEQ ID NO: 27, comprising a G at position 298;
SEQ ID NO: 28, comprising a G at position 483;
SEQ ID NO: 29, comprising an A at position 44;
SEQ ID NO: 30, comprising a C at position 101;
SEQ ID NO: 31, comprising a T at position 90;
SEQ ID NO: 32, comprising a G at position 871;
SEQ ID NO: 33, comprising a G at position 460;
SEQ ID NO: 34, comprising an A at position 252
SEQ ID NO: 35, comprising an A at position 225;
SEQ ID NO: 36, comprising a T at position 232;
SEQ ID NO: 37, comprising an A at position 321; and
SEQ ID NO: 38, comprising a G at position 101,

wherein said marker loci is associated with an LW resistance quantitative trait locus (QTL) LW_2.01;
b) selecting from said first population one or more progeny corn plants or corn seeds comprising said one or more of said marker loci linked to said LW resistance allele; and
c) producing from said selected one or more progeny corn plants or corn seeds via crossing a second progeny population of corn plants grown therefrom or corn seeds comprising said one or more of said marker loci linked to said LW resistance allele.

10. The method of claim 9 , further comprising: crossing a first LW resistant corn plant comprising said LW resistance QTL LW_2.01 with a second corn plant of a different genotype to produce said first population of corn plants or corn seeds.

11. The method of claim 9 , further comprising:
a) crossing said first progeny corn plant with itself or a plant from said second progeny population of corn plants to produce one or more further progeny plants grown therefrom or corn seeds; and
b) selecting a further progeny corn plant or corn seed comprising said one or more of said marker loci linked to said LW resistance allele.

12. The method of claim 1 , wherein said one or more of said marker loci is selected from the group consisting of:
SEQ ID NO: 8, comprising a T at position 244;
SEQ ID NO: 9, comprising a G at position 277;
SEQ ID NO: 10, comprising an A at position 430;
SEQ ID NO: 11, comprising a C at position 269;
SEQ ID NO: 12, comprising a G at position 61;
SEQ ID NO: 13, comprising an A at position 61;
SEQ ID NO: 14, comprising a T at position 61;
SEQ ID NO: 15, comprising a T at position 138;
SEQ ID NO: 16, comprising a C at position 61;
SEQ ID NO: 17, comprising an A at position 101;
SEQ ID NO: 21, comprising a C at position 148;
SEQ ID NO: 22, comprising a C at position 101;
SEQ ID NO: 23, comprising a C at position 101;
SEQ ID NO: 26, comprising an A at position 674;
SEQ ID NO: 27, comprising a G at position 298;
SEQ ID NO: 28, comprising a G at position 483;
SEQ ID NO: 29, comprising an A at position 44;
SEQ ID NO: 30, comprising a C at position 101;
SEQ ID NO: 31, comprising a T at position 90;
SEQ ID NO: 32, comprising a G at position 871;
SEQ ID NO: 33, comprising a G at position 460;
SEQ ID NO: 34, comprising an A at position 252;
SEQ ID NO: 35, comprising an A at position 225;
SEQ ID NO: 36, comprising a T at position 232;
SEQ ID NO: 37, comprising an A at position 321; and
SEQ ID NO: 38, comprising a G at position 101.

13. The method of claim 1 , wherein said second progeny corn plant or corn seeds further comprises a second LW resistance allele linked to an LW resistance QTL selected from LW_1.01, LW_1.02, LW_1.03, LW_1.04, LW_3.01, LW_3

Assignees

• Monsanto Technology Llc

Inventors

• B Rakesh
• Biradar Sunil Kumar
• Chopin Franck Jean
• Fouquet Romain
• GANDHI Sonali Dilip
• Gangappa Erappa
• Gowda Veeresh R P
• Pan Yule
• Pellet Jean-Luc
• Swamy Dharanendra
• Torres Gonzalo
• Xie Chongqing

Classifications

• A01H1/045

  using molecular markers · CPC title

• A01H6/4684

  Zea mays [maize] · CPC title

• A01H5/10

  Seeds · CPC title

• C12N15/8282

  for fungal resistance · CPC title

• C12Q1/6895 Primary

  for plants, fungi or algae · CPC title