Amino acid-specific binder and selectively identifying an amino acid

Patent metadata

Field	Value
Publication number	US-10836798-B2
Application number	US-201916395407-A
Country	US
Kind code	B2
Filing date	Apr 26, 2019
Priority date	Nov 8, 2018
Publication date	Nov 17, 2020
Grant date	Nov 17, 2020

Abstract

An amino acid-specific binder selectively binds to a binding amino acid. A binder complex selectively identifies the binding amino acid and includes an adjunct attached to the amino acid-specific binder. The adjunct includes a taggant, protein, substrate, or chemical modifier. Selectively identifying an N-terminal amino acid includes anchoring a C-terminal end; contacting an N-terminal amino acid of the anchored analyte with the binder complex; selectively binding when the N-terminal amino acid includes the binding amino acid; producing, by the taggant of the tagged complex, a taggant signal; detecting the taggant signal; and identifying the N-terminal amino acid based on the taggant signal.

First claim

What is claimed is:

1. An amino acid-specific binder for selectively binding to an amino acid in an analyte, the amino acid-specific binder comprising:

a first amino acid sequence comprising

(Sequence ID No. 1)

SDSPVDLKPKPKVKPKLERPKLYKVMLLNDDYTCPSFVTVVLKAVFRMSE

DTGRRVMMTAHRFGSAVVVVCERDIAETKAKEATDLGKEAGFPLMFTTEP

EE;

a second amino acid sequence comprising

(Sequence ID No. 2) 

SDSPVDLKPKPKVKPKLERPKLYKVMLLNDDYTCSWFVTVVLKAVFRMSE

DTGRRVMMTAHRFGSAVVVVCERDIAETKAKEATDLGKEAGFPLMFTTEP

EE;

a third amino acid sequence comprising

(Sequence ID No. 3)

SDSPVDLKPKPKVKPKLERPKLYKVMLLNDDYTPMSFVTVVLKAVFRMSE

DTGRRVMMTAHRFGSAVVVVCERDIAETKAKEATDLGKEAGFPLMFTTEP

EE;

a fourth amino acid sequence comprising

(Sequence ID No. 4)

SDSPVDLKPKPKVKPKLERPKLYKVMLLNDDYTSGRFVTVVLKAVFRMSE

DTGRRVMMTAHRFGSAVVVVCERDIAETKAKEATDLGKEAGFPLMFTTEP

EE;

a fifth amino acid sequence comprising

(Sequence ID No. 5)

SDSPVDLKPKPKVKPKLERPKLYKVMLLNDDYTPMPFVTVVLKAVFRMSE

DTGRRVMMTAHRFGSAVVVVCERDIAETKAKEATDLGKEAGFPLMFTTEP

EE;

a sixth amino acid sequence comprising

(Sequence ID No. 6)

SDSPVDLKPKPKVKPKLERPKLYKVMLLNDDYTPREFVTVVLKAVFRMSE

DTGRRVMMTAHRFGSAVVVVSERDIAETKAKEATDLGKEAGFPLMFTTEP

EE;

a seventh amino acid sequence comprising

(Sequence ID No. 7)

SDSPVDLKPKPKVKPKLERPKLYKVMLLNDDYTPREFVTEVLKAVFNMSE

DQGRRVMMTAHRFGSAVVGVCTRDIAETKAKQATDLAREAGFPLMFTTEP

EE;

an eighth amino acid sequence comprising

(Sequence ID No. 8)

SDSPVDLKPKPKVKPKLERPKLYKVMLLNDDYTPMSFVTEVLKAVFNMSE

DQGRRVMMTAHRFGSAVVGVSTRDIAETKAKQATDLAREAGFPLMFTTEP

EE;

or

a ninth amino acid sequence comprising

(Sequence ID No. 10)

NLEKIKKLRNVIKEIKKDNIKEADEHEKKEREKETSAWKVILYNDDIHKF

SYVTDVIVKVVGQISKAKAHTITVEAHSTGQALILSTWKSKAEKYCQELQ

QNGLTVSIIHESQLKDKQKK.

2. An amino acid-specific binder for selectively binding to an amino acid in an analyte, the amino acid-specific binder comprising the amino acid sequence comprising:
a first amino acid sequence comprising X1-C-P-S-X2-V-X3-R-X4-T-X5-C-E-X6-E-X7-G-K-X8 (Sequence ID No. 1);
a second amino acid sequence comprising X1-C-S-W-X2-V-X3-R-X4-T-X5-C-E-X6-E-X7-G-K-X8 (Sequence ID No. 2);
a third amino acid sequence comprising X1-P-M-S-X2-V-X3-R-X4-T-X5-C-E-X6-E-X7-G-K-X8 (Sequence ID No. 3);
a fourth amino acid sequence comprising X1-S-G-R-X2-V-X3-R-X4-T-X5-C-E-X6-E-X7-G-K-X8 (Sequence ID No. 4);
a fifth amino acid sequence comprising X1-P-M-P-X2-V-X3-R-X4-T-X5-C-E-X6-E-X7-G-K-X8 (Sequence ID No. 5);
a sixth amino acid sequence comprising X1-P-R-E-X2-V-X3-R-X4-T-X5-C-E-X6-E-X7-G-K-X8 (Sequence ID No. 6);
a seventh amino acid sequence comprising X1-P-R-E-X2-V-X3-R-X4-T-X5-S-E-X6-E-X7-G-K-X8 (Sequence ID No. 7);
an eighth amino acid sequence comprising X1-P-M-S-X2-E-X3-N-X4-Q-X5-S-T-X6-Q-X7-A-R-X8 (Sequence ID No. 8);
wherein:
X1 comprises the amino acid sequence comprising SDSPVDLKPKPKVKPKLERPKLYKVMLLNDDYT (Sequence ID No. 20);
X2 comprises the amino acid sequence comprising FVT;
X3 comprises the amino acid sequence comprising VLKAVF (Sequence ID No. 22);
X4 comprises the amino acid sequence comprising MSED (Sequence ID No. 23);
X5 comprises the amino acid sequence comprising GRRVMMTAHRFGSAVVVV (Sequence ID No. 24);
X6 comprises the amino acid sequence comprising RDIAETKAK (Sequence ID No. 25);
X7 comprises the amino acid sequence comprising ATDL (Sequence ID No. 26); and
X8 comprises the amino acid sequence comprising EAGFPLMFTTEPEE (Sequence ID No. 27),
such that a total percentage amount of substitutions and deletions to X1, X2, X3, X4, X5, X6, X7, and X8 is from 0% to less than 30%,
exclusive of

(Sequence ID No. 27)

SDSPVDLKPKPKVKPKLERPKLYKVMLLNDDYTCPSFVTVVLKAVFRMSE

DTGRRVMMTAHRFGSAVVVVC

Assignees

• Government Of The Us Secretary Of Commerce

Inventors

• Tullman Jennifer A
• Kelman Zvi
• Marino John P

Classifications

• C07K1/128

  sequencing · CPC title

• G01N33/6812

  Assays for specific amino acids · CPC title

• C07K14/445

  Plasmodium · CPC title

• C07K14/195 Primary

  from bacteria · CPC title

• C07K1/13

  Labelling of peptides · CPC title