Dual functional novel peptide having cell permeability and bone tissue regeneration ability, and use thereof

Patent metadata

Field	Value
Publication number	US-10836795-B2
Application number	US-201716464099-A
Country	US
Kind code	B2
Filing date	Dec 27, 2017
Priority date	Dec 27, 2016
Publication date	Nov 17, 2020
Grant date	Nov 17, 2020

Abstract

A peptide derived from the copine 7 protein and having both cell permeability and bone tissue regeneration ability, and a use of the peptide, are described. The peptide has excellent bone tissue regeneration ability and is therefore useful for treating a disease requiring bone regeneration, such as osteoporosis. Particularly, by also having cell permeability, the peptide does not require the attachment of a separate peptide or addition of another preparation for the cell permeation thereof and thus can be conveniently applied in orthopedics and the like requiring various surgical regeneration treatments.

First claim

The invention claimed is:

1. A peptide consisting of the amino acid sequence of SEQ ID NO: 4(VNPKYKQKRR), wherein one or more amino acid is D-type amino acid.

2. The peptide according to claim 1 , wherein the peptide has dual functions of cell permeability and bone tissue regeneration ability.

3. A method for regenerating bone tissue, the method comprising administering a pharmaceutical composition comprising a peptide consisting of the amino acid sequence of SEQ ID NO: 4 or grafting a biomaterial comprising a peptide consisting of the amino acid sequence of SEQ ID NO: 4 to a subject in need of bone tissue regeneration.

4. The method according to claim 3 , wherein the composition is formulated as the one selected from the group consisting of a formulation for oral administration, a formulation for injection, and a gel formulation for topical implantation.

5. The method according to claim 4 , wherein the gel formulation for topical implantation comprises:
i) a synthetic polymer selected from the group consisting of polylactic glycolic acid, a poloxamer and propylene glycol; or
ii) a natural polymer selected from the group consisting of collagen, alginic acid, propylene glycol alginate, chondroitin sulfate and chitosan.

6. The method according to claim 3 , wherein the composition is administered at a dose of 1 to 60 mg per 1 kg of a body weight of a subject in need of treatment.

7. A biomaterial comprising a peptide consisting of the amino acid sequence of SEQ ID NO: 4, and a chemically synthesized bone mineral or synthetic polymer.

8. The biomaterial according to claim 7 , wherein the chemically synthesized bone mineral is hydroxyapatite.

9. The biomaterial according to claim 7 , wherein the synthetic polymer is selected from the group consisting of polylactic glycolic acid, a poloxamer and propylene glycol.

Assignees

• Seoul Nat Univ R&Db Foundation
• Nano Intelligent Biomedical Eng Corporation Co Ltd

Inventors

• Park Yoon Jeong
• Chung Chong-Pyoung
• Lee Jue-Yeon
• Park Joocheol

Classifications

• C07K7/06 Primary

  having 5 to 11 amino acids · CPC title

• A61K9/06

  Ointments; Bases therefor; {Other semi-solid forms, e.g. creams, sticks, gels (composition of ointments, creams or gels A61K47/00)} · CPC title

• A61K9/0012

  Galenical forms characterised by the site of application · CPC title

• A61K38/08

  Peptides having 5 to 11 amino acids {(A61K38/043 - A61K38/046 take precedence)} · CPC title

• A61L27/12

  Phosphorus-containing materials, e.g. apatite · CPC title