Bacillus compositions and methods of use with ruminants

Patent metadata

Field	Value
Publication number	US-10835561-B2
Application number	US-201715605484-A
Country	US
Kind code	B2
Filing date	May 25, 2017
Priority date	May 25, 2016
Publication date	Nov 17, 2020
Grant date	Nov 17, 2020

Abstract

Bacillus strains, compositions and methods are disclosed for reducing growth of microorganisms in a feed. Bacillus strains, compositions and methods are disclosed for providing beneficial effects to animals, including but not limited to increasing performance of the animal.

First claim

We claim:

1. A method of improving ruminant performance, comprising:
introducing into the digestive system of one or more ruminants an effective amount of a direct fed microbial composition comprising a Bacillus strain, wherein the Bacillus strain is selected from the group consisting of: Bacillus subtilis 1104, deposited as NRRL B-67258; Bacillus subtilis 1781, deposited as NRRL B-67259; Bacillus subtilis 747, deposited as NRRL B-67257; Bacillus subtilis 1541, deposited as NRRL B-67260; Bacillus subtilis 1999, deposited as NRRL B-67318; and Bacillus subtilis 2018, deposited as NRRL B-67261,
wherein the introduction of the direct fed microbial composition decreases the diversity of Clostridia species in the one or more ruminants and further provides at least one benefit chosen from:
inhibiting a pathogen chosen from at least one of Clostridium perfringens, Clostridium bifermentans, Clostridium beijerinckii, Clostridium butyricum, Clostridium tertium , and Clostridium sordellii in the one or more ruminants;
decreasing a mortality rate of the one or more ruminants;
improving the feed efficiency of the one or more ruminants;
reducing the occurrence of hemorrhagic bowel syndrome in the one or more ruminants;
improving rumen fermentation in the one or more ruminants;
improving milk production in the one or more ruminants; and
modulating immune responses of inflammatory cytokines in systemic and intestinal immune cells in the one or more ruminants.

2. The method of claim 1 , wherein the Bacillus strains comprise B. subtilis 1781, deposited as NRRL B-67259 and B. subtilis 747, deposited as NRRL B-67257 and the introduction of the direct fed microbial composition provides the benefit of decreasing diversity of Clostridium perfringens strains in the one or more ruminants.

3. The method of claim 1 , wherein the Bacillus strains comprise B. subtilis 1781, deposited as NRRL B-67259 and B. subtilis 747, deposited as NRRL B-67257 and the introduction of the direct fed microbial composition provides the benefit of decreasing diversity of non-toxigenic clostridial strains in the one or more ruminants.

4. The method of claim 1 , wherein the Bacillus strains comprise B. subtilis 1781, deposited as NRRL B-67259 and B. subtilis 747, deposited as NRRL B-67257 and the introduction of the direct fed microbial composition provides the benefit of increasing average energy corrected milk production in the one or more ruminants when the one or more ruminants are dairy cows.

5. The method of claim 1 , wherein the Bacillus strains comprise B. subtilis 1781, deposited as NRRL B-67259; B. subtilis 747, deposited as NRRL B-67257; and B. subtilis 2018, deposited as NRRL B-67261 and the introduction of the direct fed microbial composition provides the benefit of decreasing a digestive system related mortality rate of the one or more ruminants during a period of direct fed microbial administration.

6. The method of claim 1 , wherein the introduction step includes adding the direct fed microbial composition to a ruminant feed and introducing said ruminant feed to said one or more ruminants.

Assignees

• Church & Dwight Co Inc

Inventors

• Rehberger Thomas
• O'Neill John
• Smith Alexandra
• Davis Mari Ellen
• Thompson Jesse
• Schissel Jennifer

Classifications

• C12R2001/125

  Bacillus subtilis {; Hay bacillus; Grass bacillus} · CPC title

• C12N1/205

  Bacterial isolates · CPC title

• A61K2035/115

  Probiotics · CPC title

• A23K10/18

  of live microorganisms · CPC title

• A61K35/742 Primary

  Spore-forming bacteria, e.g. Bacillus coagulans, Bacillus subtilis, clostridium or Lactobacillus sporogenes · CPC title