Detection apparatus having a microfluorometer, a fluidic system, and a flow cell latch clamp module

Patent metadata

Field	Value
Publication number	US-10830781-B2
Application number	US-201815936365-A
Country	US
Kind code	B2
Filing date	Mar 26, 2018
Priority date	Jan 11, 2016
Publication date	Nov 10, 2020
Grant date	Nov 10, 2020

Abstract

Detection apparatus includes a microfluorometer having an objective, an excitation radiation source, and a detector. The detection apparatus also includes a fluidic system for delivering reagents from a reagent cartridge to a flow cell. The fluidic system includes a manifold body having a plurality of fluidic channels configured for fluid communication between the reagent cartridge and the flow cell. The fluidic system also includes a plurality of reagent sippers. The fluidic system also includes a valve configured to mediate fluid between reagent reservoirs and the flow cell. The detection apparatus also includes a flow cell latch clamp module having a clamp cover for holding the flow cell. The objective is configured to direct excitation radiation from the radiation source to the flow cell and to direct emission from the flow cell to the detector. The microfluorometer is movable to acquire wide-field images of different areas of the flow cell.

First claim

What is claimed is:

1. A system, comprising:
a latch clamp module, comprising:
a shaft; a clamp base;
a clamp cover to hold a sample, the clamp cover rotatably coupled to the clamp base, the clamp cover comprising a rotatable arm coupled to a clamp manifold, the clamp manifold comprising a port to be in fluidic communication with the sample; and
a biasing member supported by the clamp base and comprising a spring and a post, the biasing member engaging the rotatable arm to provide a biasing force against the rotatable arm to facilitate positioning the clamp manifold such that the port is aligned and in fluidic communication with the sample,
wherein the rotatable arm comprises a base rotatably coupled to the shaft, wherein the biasing force is to press the rotatable arm against the shaft.

2. The system of claim 1 , wherein the rotatable arm extends through a cavity of a body of the clamp cover, and wherein the rotatable arm is directly connected to the clamp manifold.

3. The system of claim 1 , wherein the sample latch clamp module further comprises: a latch, a latch button, and a lever, wherein the lever is to release the latch and clamp cover when the latch button is pressed.

4. The system of claim 1 , wherein the port is to be fluidically coupled to a passage of a fluidic device, wherein the fluidic device is to hold the sample, wherein the biasing force is to facilitate positioning the clamp manifold relative to the fluidic device such that the port is aligned and fluidically coupled with the passage.

5. The system of claim 4 , wherein the fluidic device comprises a cartridge to hold the sample, and wherein the fluidic device comprises a gasket including the passage.

6. The system of claim 1 , wherein the latch clamp module further comprises: a second biasing member that provides a rotating force to rotate the rotatable arm away from the clamp base.

7. The system of claim 1 , wherein the clamp manifold comprise an inlet port and an outlet port to be in fluidic communication with the sample, wherein the biasing force against the rotatable arm is to facilitate positioning the clamp manifold such that the inlet port and the outlet port are aligned and in fluidic communication with the sample.

8. The system of claim 7 , wherein the inlet port and the outlet port are on a same side of the clamp manifold.

9. The system of claim 8 , wherein the inlet port is to be fluidically coupled to a first passage of a fluidic device, wherein the outlet port is to be fluidically coupled to a second passage of a fluidic device, wherein the fluidic device is to hold the sample, wherein the biasing force is to facilitate positioning the clamp manifold relative to the fluidic device such that the inlet port and outlet port are aligned and fluidically coupled with the first passage and second passage.

10. The system of claim 9 , wherein the sample is a flow cell.

11. The system of claim 10 , further comprising:
a fluidic system for delivering reagents from a reagent cartridge to the flow cell; and
a microfluorometer comprising an objective, an excitation radiation source, and a detector, wherein the objective is configured to direct excitation radiation from the radiation source to the flow cell and to direct emission from the flow cell to the detector.

12. The system of claim 11 , further comprising: an X-motor, a Y-motor, an X-stage, and a Y-stage, the X-motor and the Y-motor to move the X-stage and the Y-stage, respectively, in perpendicular directions with respect to each other, the microfluorometer being secured to the X-stage, the latch clamp module being secured to the Y-stage.

13. A system, comprising:
a manifold assembly comprising:
a manifold body comprising a plurality of fluidic channels for fluid communication between a reagent cartridge and an inlet of a sample; and
a manifold carriage to hold the manifold body; and

a lock assembly comprising a fluid port that is to be fluidically connected with a fluid container when the fluid container is held in an operating position, wherein the manifold assembly is to engage the lock assembly to release the fluid container from the operating position, where the fluid port is to be fluidically disconnected from the fluid container when the lock assembly is engaged by the manifold assembly, wherein the manifold carriage is to move with respect to the lock assembly and engage the lock assembly, and wherein the lock assembly comprises a roller that engages a ramp surface of the manifold carriage.

14. The system of claim 13 , wherein the manifold assembly further comprises:
a plurality of reagent sippers extending downward from ports in the manifold body, each of the reagent sippers to be placed into a reagent reservoir of the reagent cartridge; and
a valve to mediate fluid communication between the reagent reservoirs and the inlet of the sample.

15. The system of claim 13 , wherein the sample is a flow cell.

16. The system of claim 13 , further comprising: a microfluorometer comprising an objective, an excitation radiation source, and a detector.

17. A system, comprising:
a manifold assembly comprising:
a manifold body comprising a plurality of fluidic channels for fluid communication between a reagent cartridge and an inlet of a sample; and
a manifold carriage to hold the manifold body; and

a lock assembly comprising a fluid port that is to be fluidically connected with a fluid container when the fluid container is held in an operating position, wherein the manifold assembly is to engage the lock assembly to release the fluid container from the operating position, where the fluid port is to be fluidically disconnected from the fluid container when the lock assembly is engaged by the manifold assembly, wherein the manifold carriage is to move with respect to the lock assembly and engage the lock assembly, wherein the lock assembly comprises a movable arm, wherein the movable arm is positioned away from the fluid container when the manifold assembly is engaged with the lock assembly, and wherein the movable arm is to prevent removal of the fluid container when the manifold assembly is disengaged from the lock assembly.

Assignees

• Illumina Inc
• Illumina Singapore Pte Ltd

Inventors

• Ang Beng Keong
• Cheng Heng Kuang
• Beierle John M
• Drews Bradley Kent
• Kaplan David

Classifications

• G01N21/645 Primary

  Specially adapted constructive features of fluorimeters · CPC title

• G01N35/00732

  Identification of carriers, materials or components in automatic analysers · CPC title

• G01N21/6486

  Measuring fluorescence of biological material, e.g. DNA, RNA, cells (G01N21/6428 takes precedence) · CPC title

• G01N2021/6482

  Sample cells, cuvettes · CPC title

• G01N2035/00356

  Holding samples at elevated temperature (incubation) · CPC title