Temporary storage of sample portions for artefact-preventing fluid processing

Patent metadata

Field	Value
Publication number	US-10830739-B2
Application number	US-201515500706-A
Country	US
Kind code	B2
Filing date	May 28, 2015
Priority date	Jul 31, 2014
Publication date	Nov 10, 2020
Grant date	Nov 10, 2020

Abstract

In a method for processing successive fluidic sample portions provided by a sample source, sample reception volumes are filled successively temporarily with at least a respective one of the sample sections, and the sample sections are emptied successively out of the sample reception volumes in such a way, that, while emptying, it is avoided to bring two respective ones of the sample sections, which have not left the sample source directly adjacent to one another, in contact with one another.

First claim

The invention claimed is:

1. A method for processing successive fluidic sample sections provided by a sample source configured to chromatographically separate a fluidic sample into sample fractions, the method comprising:
receiving the sample sections from the sample source, wherein one or more of the sample sections contain, respectively, one or more sample fractions separated in the sample source and detectable as peaks by a detector;
successively temporarily filling in a controlled filling sequence more than two sample reception volumes with at least a respective one of the sample sections, wherein the sample reception volumes are defined by a plurality of respective, separate fluid line sections; and
successively emptying the sample sections out of the sample reception volumes in a controlled emptying sequence by selecting an order of the emptying of the sample sections out of the sample reception volumes in a reverse order to the controlled filling sequence such that the lastly filled sample reception volumes are emptied at first and provided for further separation of the one or more sample fractions;
wherein the successively temporarily filling comprises operating a fluidic switch to selectively couple the fluid line sections with the sample source according to the controlled filling sequence, and the successively emptying comprises operating the fluidic switch to selectively couple the fluid line sections with a downstream flow path according to the controlled emptying sequence.

2. The method according to claim 1 , wherein the successively emptying of the sample sections out of the sample reception volumes is effected such that it is avoided for components of a sample section, which is, prior to the filling, not adjacent to a sample section that has just been emptied, to remain in feed lines and to impurify, to contaminate or to lead to a sample carry-over to the sample section that has just been emptied.

3. The method according to claim 1 , wherein the filling and the emptying of the sample reception volumes is controlled by switching at least one fluid valve.

4. The method according to claim 3 , wherein at least one of the fluid line sections is between two respective ports of the at least one fluid valve.

5. The method according to claim 1 , wherein the successively emptying the sample sections out of the sample reception volumes is effected such that, while emptying, a direct bringing-in-contact of different sample sections is made impossible.

6. The method according to claim 1 , wherein in the sample source, the fluidic sample is separated in the sample sections.

7. The method according to claim 1 , comprising a feature selected from the group consisting of:
the method further comprises, after the emptying, successively conveying the sample sections into a fluidic path between a fluid conveying device for conveying a mobile phase and a fluid separation device for further separating the sample sections into the one or more sample fractions;
the successively emptying of the sample sections out of the sample reception volumes is effected such that, while emptying, it is actively avoided to bring two respective ones of the sample sections, which have not left the sample source directly adjacent to one another, in contact with one another;
two respective ones of the sample sections, which have left the sample source directly adjacent to one another, adjoin directly physically, without a medium arranged therebetween, with one another at a fluidic outlet of the sample source; and
a combination of two or more of the foregoing.

8. The method according to claim 1 , further comprising:
assigning assignment information to at least one of the sample sections, wherein the assignment information is indicative for a rank of the respective sample section in at least one of a ranking order of the filling of the sample reception volumes with the sample sections or a ranking order of the emptying of the sample sections out of the sample reception volumes; and
controlling a further processing of the sample sections on the basis of the assignment information.

9. A fluid processing apparatus for processing successive fluidic sample sections provided by a sample source configured to chromatographically separate a fluidic sample into sample fractions, the fluid processing apparatus comprising:
more than two sample reception volumes, which are configured to be filled successively temporarily with at least a respective one of the sample sections in a controlled filling sequence, wherein one or more of the sample sections contain, respectively, one or more sample fractions separated in the sample source and detectable as peaks by a detector, and the sample reception volumes are defined by a plurality of respective, separate fluid line sections;
a control device configured to successively empty the sample sections out of the sample reception volumes in a controlled emptying sequence by selecting an order of the emptying of the sample sections out of the sample reception volumes in a reverse order to the controlled filling sequence such that the lastly filled sample reception volumes are emptied at first and provided for further separation of the one or more sample fractions; and
a fluidic switch, wherein the control device is configured to successively temporarily fill the sample reception volumes with the sample sections by controlling the fluidic switch to selectively couple the fluid line sections with the sample source according to the controlled filling sequence, and the control device is configured to successively empty the sample sections out of the sample reception volumes by controlling the fluidic switch to selectively couple the fluid line sections with a downstream flow path according to the controlled emptying sequence.

10. The fluid processing apparatus of claim 9 , comprising:
an assignment device configured to assign assignment information to at least one of the sample sections, wherein the assignment information is indicative for a rank of the respective sample section in at least one of a ranking order of the filling of the sample reception volumes with the sample sections or a ranking order of the emptying of the sample sections out of the sample reception volumes;
wherein the control device is configured to control a further processing of the sample sections on the basis of the assignment information.

11. A system for processing a fluidic sample, the system comprising:
the fluid processing apparatus of claim 9 ; and
the sample source.

12. The system according to claim 11 , wherein:
the sample source is configured as a first separation stage for separating the fluidic sample into the sample sections, wherein at least one of the sample sections has at least one fraction of the fluidic sample; and
the fluid processing apparatus is configured as a second separation stage for separating at least one of the fractions of the sample sections into partial fractions.

13. The fluid processing apparatus of claim 9 , wherein the fluidic switch comprises a fluidic valve comprising a plurality of ports, and the fluid line sections comprise respective sample loops coupled between respective pairs of ports of the plurality of ports.

14. A method for processing successive fluidic sample sections provided by a sample source configured to chromatographically separate a fluidic sample into sample fractions, the method comprising:
receiving the sample sections from the sample source, wherein one or more of the sample sections contain, respectively, one or more sample fractions separated in the sample source and detectable as peaks by a detect

Assignees

• Agilent Technologies Inc

Inventors

• Anderer Herbert
• Shoykhet Konstantin
• Witt Klaus
• Buckenmaier Stephan

Classifications

• G01N2030/445

  heart cut · CPC title

• G01N30/44 Primary

  using recycling of the fraction to be distributed · CPC title

• B01D15/1878

  for multi-dimensional chromatography · CPC title

• G01N30/465

  with specially adapted interfaces between the columns · CPC title

• B01D15/242

  Intermediate storage of effluents · CPC title