Electrode and use thereof

Patent metadata

Field	Value
Publication number	US-10830727-B2
Application number	US-201715803267-A
Country	US
Kind code	B2
Filing date	Nov 3, 2017
Priority date	May 1, 2012
Publication date	Nov 10, 2020
Grant date	Nov 10, 2020

Abstract

The application relates to an electrode for use in the electrochemical detection of a target species, wherein the electrode has a planar surface disposed on which are probe molecules that are capable of binding selectively to the target species, wherein the electrode, prior to binding of the probe molecules with the target species, has an electron transfer resistance per area of the electrode of from 10 megaohms cm−2 to 95 megaohms cm−2.

First claim

We claim:

1. An electrode for use in the electrochemical detection of a target species, the electrode comprising:
a planar surface having probe molecules disposed thereon, in which the probe molecules are capable of electrochemically detecting a target species by binding selectively to the target species,
wherein the probe molecule is of the formula A-L-B, where A is a moiety that binds to the surface of the electrode, L is a linker moiety and B is a moiety which binds selectively to the target species;
wherein the electrode, prior to binding of the probe molecules with the target species, has an electron transfer resistance per area of the electrode of from 10 megaohms cm −2 to 95 megaohms cm −2 , and
wherein the target species is an antigen or analyte selected from the group consisting of angiotensin I converting enzyme (peptidyl-dipeptidase A) 1; adiponectin; advanced glycosylation end product-specific receptor; alpha-2-HS-glycoprotein; angiogenin, ribonuclease, RNase A family, 5; apolipoprotein A-1; apolipoprotein B (including Ag(x) antigen); apolipoprotein E; BCL2-associated X protein; B-cell CLL/lymphoma 2; complement C3; chemokine (C-C motif) ligand 2;

CD 14, soluble; CD 40, soluble; cdk5; pentraxin-related; cathepsin B; dipeptidyl peptidase IV; Epidermal growth factor; endoglin; Fas; fibrinogen; ferritin; growth hormone 1; alanine aminotransferase; hepatocyte growth factor; haptoglobin; heat shock 70 kDa protein 1 B; intercellular adhesion molecule 1; insulin-like growth factor 1 (somatomedin C); insulin-like growth factor 1 receptor; insulin-like growth factor binding protein 1; insulin-like growth factor binding protein 2; insulin-like growth factor-binding protein 3; interleukin 18; interleukin 2 receptor, alpha; interleukin 2 receptor, beta; interleukin 6 (interferon, beta 2); interleukin 6 receptor; interleukin 6 signal transducer (gp130, oncostatin M receptor); interleukin 8; activin A; leptin (obesity homolog, mouse); plasminogen activator, tissue; proopiomelanocortin (adrenocorticotropin/beta-lipotropin/alpha-melanocyte stimulating hormone/beta-melanocyte stimulating hormone/beta-endorphin); proinsulin; resistin; selectin e (endothelial adhesion molecule 1); selectin P (granule membrane protein 140 kDa, antigen CD62); serpin peptidase inhibitor, Glade E (nexin, plasminogen activator inhibitor type 1), member 1; serum/glucocorticoid regulated kinase; sex hormone-binding globulin; transforming growth factor, beta 1 (Camurati-Engelmann disease); TIMP metallopeptidase inhibitor 2; tumor necrosis factor receptor superfamily, member 1 B; vascular cell adhesion molecule 1 (VCAM-1); vascular endothelial growth factor; Factor II, Factor V, Factor VIII, Factor IX, Factor XI, Factor XII, F/fibrin degradation products, thrombin-antithrombin III complex, fibrinogen, plasminogen, prothrombin, and von Willebrand factor; markers useful for diabetes include C-reactive protein; glucose; insulin; TRIG; GPT; HSPA1 B; IGFBP2; LEP; ADIPOQ; CCL2; ENG;
HP; IL2RA; SCp; SHBG; and TIMP2.

2. An electrode according to claim 1 , wherein the probe molecules comprise at least one of: antibodies or antibody fragments.

3. An electrode according to claim 1 , wherein the electrode prior to binding of the probe molecules with the target species has an electron transfer resistance per area of the electrode of from 55 megaohms cm −2 to 95 megaohms cm′.

4. An electrode for use according to claim 1 , wherein the electrode comprises a metal selected from the group consisting of: gold, rhenium, iridium, palladium, platinum, copper, indium, rubidium silver, and combinations thereof.

5. An electrode according to claim 1 , wherein A is selected from the group consisting of: biotin, hydrazine, alkynyl, alkylazide, amino, hydroxyl, carboxy, thiol, aldehyde, phosphoinothioester, maleimidyl, succinyl, succinimidyl, isocyanate, ester, strepavidin, avidin, neuavidin, biotin binding proteins, and combinations thereof.

6. An electrode according to claim 1 , wherein the probe molecule is of the formula A-L-B, where A is a moiety that binds to the surface of the electrode, L is a linker moiety and B is a moiety which binds selectively to the target species, and L comprises an alkylene moiety comprising at least 10 carbons in which L is of the formula —(CH 2 ) n -(—O—CH 2 —CH 2 —) m -D-, wherein n is from 1 to 30 and m is from 0 to 10 and D is a group that binds to B.

7. An electrode for use in the electrochemical detection of a target species, the electrode comprising:
a planar surface having probe molecules disposed thereon, in which the probe molecules are capable of electrochemically detecting a target species by binding selectively to the target species, wherein the electrode, prior to binding of the probe molecules with the target species, has an electron transfer resistance per area of the electrode of from 10 megaohms cm −2 to 95 megaohms cm −2 ,
wherein the target species is an antigen or analyte that is indicative of a particular disease, and
wherein the probe molecule is of the formula A-L-B, where A is a moiety that binds to the surface of the electrode, L is a linker moiety and B is a moiety which binds selectively to the target species, and L comprises an alkylene moiety comprising at least 10 carbons.

8. An electrode according to claim 7 , wherein the target species is an antigen or analyte selected from the group consisting of angiotensin I converting enzyme (peptidyl-dipeptidase A) 1; adiponectin; advanced glycosylation end product-specific receptor; alpha-2-HS-glycoprotein; angiogenin, ribonuclease, RNase A family, 5; apolipoprotein A-1; apolipoprotein B (including Ag(x) antigen); apolipoprotein E; BCL2-associated X protein; B-cell CLL/lymphoma 2;
complement C3; chemokine (C-C motif) ligand 2; CD 14, soluble; CD 40, soluble; cdk5; pentraxin-related; cathepsin B; dipeptidyl peptidase IV; Epidermal growth factor; endoglin; Fas; fibrinogen; ferritin; growth hormone 1; alanine aminotransferase; hepatocyte growth factor; haptoglobin; heat shock 70 kDa protein 1 B; intercellular adhesion molecule 1; insulin-like growth factor 1 (somatomedin C); insulin-like growth factor 1 receptor; insulin-like growth factor binding protein 1;
insulin-like growth factor binding protein 2; insulin-like growth factor-binding protein 3; interleukin 18; interleukin 2 receptor, alpha; interleukin 2 receptor, beta; interleukin 6 (interferon, beta 2); interleukin 6 receptor; interleukin 6 signal transducer (gp130, oncostatin M receptor); interleukin 8;
activin A; leptin (obesity homolog, mouse); plasminogen activator, tissue; proopiomelanocortin (adrenocorticotropin/b eta-lip otropin/alpha-mel anocyte stimulating hormone/beta-melanocyte stimulating hormone/beta-endorphin); proinsulin; resistin; selectin e (endothelial adhesion molecule 1); selectin P (granule membrane protein 140 kDa, antigen CD62); serpin peptidase inhibitor, Glade E(nexin, plasminogen activator inhibitor type 1), member 1; serum/glucocorticoid regulated kinase; sex hormone-binding globulin; transforming growth factor, beta 1 (Camurati-Engelmann disease); TIMP metallopeptidase inhibitor 2; tumor necrosis factor receptor superfamily, member 1 B; vascular cell adhesion molecule 1 (VCAM-1); vascular endothelial growth factor; Factor II, Factor V, Factor VIII, Factor IX, Factor XI, Factor XII, F/fibrin degradation products, thrombin-antithrombin III complex, fibrinogen, plasminogen, prothrombin, and von Willebrand factor and the like; markers useful for diabetes include C-reactive protein; glucose; insulin; TRIG; GPT; HSPA1 B;
IGFBP2; LEP; ADIPOQ; CCL2; ENG; HP; IL2RA; SCp; SHBG; and TIMP2.

9. A method for detecting a target species in an electrochemical impedance spectroscopy technique, where

Assignees

• Univ Oxford Innovation Ltd
• Univ Estadual Paulista “Julio De Mesquita Filho”
• Univ Estadual Paulista “Julio De Mequita Filho”

Inventors

• Davis Jason
• Bueno Paulo Roberto

Classifications

• G01N33/5438 Primary

  Electrodes · CPC title

• G01N27/3276

  being a hybridisation with immobilised receptors (using a FET type sensor G01N27/4145; concerning the hybridisation C12Q1/68) · CPC title

• G01N2333/00

  Assays involving biological materials from specific organisms or of a specific nature · CPC title

• G01N27/327 Primary

  Biochemical electrodes {, e.g. electrical or mechanical details for in vitro measurements} · CPC title

• G01N2333/4737

  C-reactive protein · CPC title