Method for the fermentative production of L-lysine

Patent metadata

Field	Value
Publication number	US-10829746-B2
Application number	US-201916718779-A
Country	US
Kind code	B2
Filing date	Dec 18, 2019
Priority date	Jan 23, 2019
Publication date	Nov 10, 2020
Grant date	Nov 10, 2020

Abstract

A bacterium of the species Corynebacterium glutamicum has the ability to excrete L-lysine, and contains in its chromosome a polynucleotide encoding a mutated polypeptide having the assumed function of an acyltransferase, hydrolase, alpha/beta hydrolase or of a pimeloyl-ACP methyl ester esterase. Also, a method is used for producing L-lysine using such bacterium.

First claim

The invention claimed is:

1. A bacterium of the species Corynebacterium glutamicum , comprising:
an ability to excrete L-lysine, and
a polynucleotide encoding a polypeptide comprising the amino acid sequence of SEQ ID NO: 2, except the serine at the position corresponding to position 225 of the amino acid sequence of SEQ ID NO: 2 is substituted with a different amino acid, and wherein the polynucleotide is contained in the chromosome of the bacterium.

2. The bacterium of claim 1 , wherein the serine at the position corresponding to position 225 of the amino acid sequence of SEQ ID NO: 2 is substituted with cysteine.

3. The bacterium of claim 2 , wherein the polynucleotide comprises the nucleotide sequence of nucleotides 328 to 1320 of SEQ ID NO: 1 with guanine at the position corresponding to position 1001 of SEQ ID NO: 1.

4. The bacterium of claim 2 , wherein the polynucleotide comprises the nucleotide sequence of nucleotides 328 to 1323 of SEQ ID NO: 1 with guanine at the position corresponding to position 1001 of SEQ ID NO: 1.

5. The bacterium of claim 1 , comprising at least one copy of a polynucleotide comprising a nucleotide sequence encoding a feedback resistant aspartokinase polypeptide, wherein the feedback resistant aspartokinase polypeptide comprises the amino acid sequence of SEQ ID NO: 6.

6. The bacterium of claim 1 , comprising at least one copy of a polynucleotide comprising a nucleotide sequence encoding for a feedback resistant aspartokinase polypeptide comprising the amino acid sequence of SEQ ID NO: 6, except the threonine at the position corresponding to position 311 of the amino acid sequence of SEQ ID NO: 6 is substituted with isoleucine.

7. A method for fermentative production of L-lysine, comprising:
a) cultivating the bacterium of claim 1 in a suitable medium under suitable conditions for the production of L-lysine, and
b) accumulating L-lysine in the suitable medium to form an L-lysine containing fermentation broth.

8. The method of claim 7 , further comprising:
purifying L-lysine.

9. The method of claim 8 , wherein L-lysine is purified by at least one process selected from the group consisting of treatment with activated carbon, ionic exchange, and crystallization.

Assignees

• Evonik Operations Gmbh

Inventors

• Schneider Frank
• Thierbach Georg
• Voss Kornelia
• Bekel Thomas

Classifications

• C12N9/1025 Primary

  Acyltransferases (2.3) · CPC title

• C12P13/08

  Lysine; Diaminopimelic acid; Threonine; Valine · CPC title

• C12Y207/02004

  Aspartate kinase (2.7.2.4) · CPC title

• C07C227/40

  Separation; Purification · CPC title

• C12Y301/01085 Primary

  Pimelyl-[acyl-carrier protein] methyl ester esterase (3.1.1.85) · CPC title