Implant materials for TMJ repair, methods of making the implant materials for TMJ repair, and method of using implant materials for TMJ repair

Patent metadata

Field	Value
Publication number	US-10828165-B2
Application number	US-201214363538-A
Country	US
Kind code	B2
Filing date	Dec 7, 2012
Priority date	Dec 9, 2011
Publication date	Nov 10, 2020
Grant date	Nov 10, 2020

Abstract

In accordance with the purpose(s) of the present disclosure, as embodied and broadly described herein, embodiments of the present disclosure, in one aspect, relate to TMJ implantation materials and implants (e.g., temporomandi bular joint (TMJ) methods of making TMJ implantation materials and implants, methods of forming a TMJ implantation material or an implant, and the like.

First claim

I claim:

1. A method of forming a temporomandibular joint (TMJ) disc, comprising the steps of:
obtaining a precursor TMJ scaffold structure comprising a fibrocartilage tissue from a subject animal;
decellularizing the precursor TMJ scaffold structure to produce a decellularized TMJ scaffold structure;
freeze drying the decellularized TMJ scaffold structure to form a freeze-dried TMJ scaffold structure, such that the freeze-drying process produces a decellularized, freeze-dried fibrocartilage tissue having a compressive modulus and a peak stress value less than that of a corresponding sodium dodecyl sulfate (SDS) decellularized fibrocartilage tissue that has not been freeze-dried; and
after freeze drying, drilling a plurality of drilled holes in the decellularized, freeze-dried TMJ scaffold structure.

2. The method of claim 1 , further comprising:
culturing the freeze-dried TMJ scaffold structure under conditions whereby the freeze-dried TMJ scaffold structure is colonized by a population of cells, wherein a portion of the cells are in the holes; and
rehydrating the freeze-dried TMJ scaffold structure to form a TMJ scaffold structure.

3. The method of claim 2 , wherein during the culturing and rehydration process a portion of the cells enter the interior of the TMJ scaffold structure.

4. The method of claim 1 , wherein the decellularized TMJ scaffold structure is substantially free of non-TMJ tissue.

5. The method of claim 1 , wherein decellularizing includes contacting the precursor TMJ scaffold structure with sodium dodecyl sulphate or sodium chloride.

6. The method of claim 2 , wherein the cells are selected from the group consisting of: a stem cell, an endothelial cell, a smooth muscle cell, a fibroblast, and a combination thereof.

7. The method of claim 2 , wherein the population of cells is a homogeneous population of cells.

8. The method of claim 1 , wherein the population of cells is a heterogenous population of cells.

9. The method of claim 1 , wherein the holes have a diameter of about 1 to 500 micrometers.

10. The method of claim 1 , wherein one or more pairs of holes are spaced by about 1 micrometer to 10 millimeters.

11. The method of claim 1 , wherein the holes are through the scaffold structure, the holes are only partially through the scaffold structure, or a portion of the holes are through the scaffold structure and a portion of the holes are only partially through the scaffold structure.

12. The method of claim 1 , wherein the TMJ scaffold structure is a porcine TMJ fibrocartilage tissue.

13. The method of claim 1 , wherein the fibrocartilage tissue comprises a TMJ disc from the subject animal.

14. The method of claim 1 , wherein the subject animal is a mammal selected from the group of mammals consisting of: humans, porcines, ovines, and bovines.

15. The method of claim 1 , wherein the fibrocartilage tissue is a xenogeneic fibrocartilage tissue.

Assignees

• Univ Florida

Inventors

• Mcfetridge Peter S

Classifications

• A61L27/386

  Ligaments, tendons · CPC title

• A61L27/3662

  Ligaments, tendons · CPC title

• A61L27/3886

  comprising two or more cell types · CPC title

• A61L2430/10

  for reconstruction of tendons or ligaments · CPC title

• A61L2430/40

  Preparation and treatment of biological tissue for implantation, e.g. decellularisation, cross-linking · CPC title