Modifier stream elution of trap column for multidimensional compressible fluid-based chromatography

Patent metadata

Field	Value
Publication number	US-10823712-B2
Application number	US-201715850146-A
Country	US
Kind code	B2
Filing date	Dec 21, 2017
Priority date	Jan 6, 2017
Publication date	Nov 3, 2020
Grant date	Nov 3, 2020

Abstract

A method of performing a chromatographic separation includes modulating a portion of a flowstream to a trap column to retain at least one analyte from the flowstream on the trap column. A flow of a modifier is provided through the trap column to generate an elution comprising the at least one analyte. A flow of a compressible fluid-based chromatography (CFC) mobile phase or CFC solvent is merged with the elution from the trap column to generate a diluted elution. Carbon dioxide may be used as the CFC solvent or as a component of the CFC mobile phase. The diluted elution is provided to a CFC column where at least one analyte is focused at a head of the CFC column. Examples of a flowstream that may be used include an eluent from a chromatography column or a fluid flow from an extraction system.

First claim

The invention claimed is:

1. A method of performing a chromatographic separation, the method comprising:
modulating a portion of a flowstream to a trap column to thereby retain at least one analyte from the flowstream on the trap column;
providing a flow of a solvent through the trap column to thereby generate an elution comprising the at least one analyte, the solvent being only one of at least two different solvents mixed to form a compressible fluid based chromatography (CFC) solvent;
merging a flow of the CFC solvent with the elution from the trap column to generate a diluted elution; and
providing the diluted elution to a CFC column, wherein the at least one analyte is focused at a head of the CFC column.

2. The method of claim 1 wherein the flowstream comprises an eluent from a chromatography system.

3. The method of claim 1 wherein the merging of the flow of the CFC solvent with the elution from the trap column comprises merging a flow of carbon dioxide with the elution from the trap column.

4. The method of claim 1 wherein the CFC solvent is a CFC mobile phase.

5. The method of claim 4 wherein the CFC mobile phase comprises carbon dioxide.

6. The method of claim 5 wherein the CFC mobile phase further comprises a modifier.

Assignees

• Waters Technologies Corp

Inventors

• Fogwill Michael O
• François Isabelle

Classifications

• G01N30/463 Primary

  for multidimensional chromatography · CPC title

• G01N30/465

  with specially adapted interfaces between the columns · CPC title

• G01N30/462

  with different eluents or with eluents in different states (G01N30/463 takes precedence) · CPC title

• G01N30/20

  using a sampling valve · CPC title

• G01N30/34

  of fluid composition, e.g. gradient (G01N30/36 takes precedence) · CPC title