Computational design of self-assembling cyclic protein homo-oligomers

Patent metadata

Field	Value
Publication number	US-10818377-B2
Application number	US-201715813872-A
Country	US
Kind code	B2
Filing date	Nov 15, 2017
Priority date	Nov 16, 2016
Publication date	Oct 27, 2020
Grant date	Oct 27, 2020

Abstract

Described herein are polypeptides capable of self-assembling to form homo-oligomers, and methods for designing such polypeptides.

First claim

We claim:

1. An isolated polypeptide comprising the amino acid sequence that has at least 70% sequence identity to the amino acid sequence of SEQ ID NO: 40, wherein the polypeptide is capable of self-assembly into homo-oligomers.

2. The isolated polypeptide of claim 1 , wherein the polypeptide comprises the amino acid sequence having at least 75% sequence identity to the amino acid sequence of SEQ ID NO: 40.

3. The isolated polypeptide of claim 1 , wherein the polypeptide comprises the amino acid sequence having at least 90% sequence identity to the amino acid sequence of SEQ ID NO: 40.

4. The isolated polypeptide of claim 1 , wherein the polypeptide comprises the amino acid sequence having at least 95% sequence identity to the amino acid sequence of SEQ ID NO: 40.

5. The isolated polypeptide of claim 1 , wherein the polypeptide comprises the amino acid sequence having at least 98% sequence identity to the amino acid sequence of SEQ ID NO: 40.

6. The polypeptide of claim 1 , wherein the polypeptide comprises the amino acid sequence selected from the group consisting of SEQ ID NOS: 16-21, 34-35, 37, and 40.

7. The isolated polypeptide of claim 1 , wherein the polypeptide comprises the amino acid sequence of SEQ ID NO: 40.

8. The isolated polypeptide of claim 1 , wherein residues in bold font for SEQ ID NO: 40 are conserved in the polypeptide EEAELAYLLGELAYKLGEYRIAIRAYRIALKRDPNNAEAWYNLGNAYYKQGRYREAIEYYQKAL ELDPNNAEAWYNLGNAYYERGEYEEAIEYYRKALRLDPNNADAMQNLLNAKMREE (SEQ ID NO: 40).

9. An isolated polypeptide homo-oligomer comprising a plurality of polypeptides according to claim 8 .

10. The isolated polypeptide homo-oligomer of claim 9 , further comprising a plurality of polypeptides having at least 97% sequence identity to the amino acid sequence of SEQ ID NO: 39.

11. An isolated polypeptide homo-oligomer comprising a plurality of polypeptides according to claim 1 .

12. The isolated polypeptide homo-oligomer of claim 11 , further comprising a plurality of polypeptides having at least 97% sequence identity to the amino acid sequence of SEQ ID NO: 39.

13. The isolated polypeptide oligomer of claim 12 , wherein residues in bold font for SEQ ID NO: 39 are conserved

(SEQ ID NO: 39)

KYDGSKLRIGIL H A R W NAE II L AL VL GA L KRL Q EFGVK R ENII IET VPGSF

ELPYGSKLFVEKQKRLGKPLDAIIPIGVLIKGSTMHFEYICDSTTHQLMKL

NFELGIPVIFGVLTCLTDEQAEARAGLIEGKMHNHGEDWGAAAVEMATKF

N.

Assignees

• Univ Washington

Inventors

• Baker David
• Fallas Jorge
• Ueda george
• Sheffler William H
• Nguyen Vanessa

Classifications

• A61K38/03 Primary

  Peptides having up to 20 amino acids in an undefined or only partially defined sequence; Derivatives thereof · CPC title

• G16B5/00 Primary

  ICT specially adapted for modelling or simulations in systems biology, e.g. gene-regulatory networks, protein interaction networks or metabolic networks · CPC title

• C07K14/00

  Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof · CPC title

• G16B15/30

  Drug targeting using structural data; Docking or binding prediction · CPC title