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Microorganism of the genus Escherichia producing L-tryptophan and method for producing L-tryptophan using the same
US10815510B2 · US · B2
| Field | Value |
|---|---|
| Publication number | US-10815510-B2 |
| Application number | US-201515321717-A |
| Country | US |
| Kind code | B2 |
| Filing date | Jun 23, 2015 |
| Priority date | Jun 23, 2014 |
| Publication date | Oct 27, 2020 |
| Grant date | Oct 27, 2020 |
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Abstract
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The present application relates to a microorganism of the genus Escherichia producing L-tryptophan and, more specifically, to a microorganism of the genus Escherichia with improved activity of producing L-tryptophan by weakening or inactivating the activity of endogenous 6-phosphogluconate dehydratase and 2-keto-3-deoxy-6-phosphogluconate aldolase. Additionally, the present application relates to a method for producing L-tryptophan using the microorganism of the genus Escherichia.
First claim
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The invention claimed is: 1. A microorganism of the genus Escherichia producing L-tryptophan, wherein the activities of endogenous 6-phosphogluconate dehydratase (Edd) and 2-keto-3-deoxy-6-phosphogluconate aldolase (Eda) are weakened or inactivated, wherein the microorganism has a weakened or blocked Entner-Doudoroff pathway thereby does not lose 6-phosphogluconate, and wherein an entirety or a part of the pheA gene, trpR gene, mtr gene, and tnaAB gene is further deleted. 2. The microorganism according to claim 1 , wherein the 6-phosphogluconate dehydratase has the amino acid sequence set forth in SEQ ID NO: 1. 3. A method of preparing L-tryptophan, comprising: culturing the microorganism of the genus Escherichia of claim 2 in a medium; and recovering L-tryptophan from the cultured medium or the cultured microorganism. 4. The microorganism according to claim 1 , wherein the 2-keto-3-deoxy-6-phosphogluconate aldolase has the amino acid sequence set forth in SEQ ID NO: 3. 5. A method of preparing L-tryptophan, comprising: culturing the microorganism of the genus Escherichia of claim 4 in a medium; and recovering L-tryptophan from the cultured medium or the cultured microorganism. 6. The microorganism according to claim 1 , wherein the microorganism of the genus Escherichia is Escherichia coli. 7. A method of preparing L-tryptophan, comprising: culturing the microorganism of the genus Escherichia of claim 6 in a medium; and recovering L-tryptophan from the cultured medium or the cultured microorganism. 8. A method of preparing L-tryptophan, comprising: culturing the microorganism of the genus Escherichia of claim 1 in a medium; and recovering L-tryptophan from the cultured medium or the cultured microorganism. 9. A method of preparing L-tryptophan, comprising: culturing a microorganism of the genus Escherichia producing L-tryptophan, wherein the activities of endogenous 6-phosphogluconate dehydratase (Edd) and 2-keto-3-deoxy-6-phosphogluconate aldolase (Eda) are weakened or inactivated, wherein the microorganism has a blocked Entner-Doudoroff pathway thereby does not lose 6-phosphogluconate in a medium, and wherein the microorganism has higher L-tryptophan production relative to an identical microorganism with the proviso the Edd and Eda are not weakened or inactivated; and recovering L-tryptophan from the cultured medium or the cultured microorganism. 10. The method according to claim 9 , wherein the microorganism of the genus Escherichia is Escherichia coli.
Assignees
Inventors
Classifications
- C12N9/88Primary
Lyases (4.) · CPC title
Phosphogluconate dehydratase (4.2.1.12) · CPC title
2-Dehydro-3-deoxy-phosphogluconate aldolase (4.1.2.14) · CPC title
- C12P13/227Primary
Tryptophan · CPC title
Tryptophan; Tyrosine; Phenylalanine; 3,4-Dihydroxyphenylalanine · CPC title
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- What does patent US10815510B2 cover?
- The present application relates to a microorganism of the genus Escherichia producing L-tryptophan and, more specifically, to a microorganism of the genus Escherichia with improved activity of producing L-tryptophan by weakening or inactivating the activity of endogenous 6-phosphogluconate dehydratase and 2-keto-3-deoxy-6-phosphogluconate aldolase. Additionally, the present applicatio…
- Who is the assignee on this patent?
- Cj Cheiljedang Corp
- What technology area does this patent fall under?
- Primary CPC classification C12N9/88. Mapped technology areas include Chemistry & Metallurgy.
- When was this patent published?
- Publication date Tue Oct 27 2020 00:00:00 GMT+0000 (Coordinated Universal Time) (B2). Legal status and post-grant events are not shown on this page.
- What related patents are in patentsdb?
- We list 8 related publications on this page (citations in our corpus or others sharing the same primary CPC).