Mildew resistance gene in kalanchoe

Patent metadata

Field	Value
Publication number	US-10815466-B2
Application number	US-201615553199-A
Country	US
Kind code	B2
Filing date	Feb 29, 2016
Priority date	Mar 4, 2015
Publication date	Oct 27, 2020
Grant date	Oct 27, 2020

Abstract

Provided herein are proteins capable of providing a mildew resistant phenotype, or mildew resistance, to plants belonging to the Kalanchoe genus. Also provided herein are nucleic acid sequences, or cDNA sequences, and genes encoding the present proteins. Further provided herein are methods using the proteins, nucleic acid sequences and genes for selecting mildew resistant Kalanchoe plants, such as Oidium kalanchoeae resistant Kalanchoe plants, and to generate mildew resistant Kalanchoe plants, such as Oidium kalanchoeae resistant Kalanchoe plants, comprising the present proteins, mRNA forms of the present cDNAs or the present genes.

First claim

The invention claimed is:

1. A protein having the sequence of SEQ ID NO: 2.

2. A method for obtaining a Kalanchoe plant that is resistant to mildew, comprising introducing a nucleic acid sequence encoding SEQ ID NO: 2 into a Kalanchoe plant that is susceptible to mildew.

3. The method according to claim 2 , wherein the Kalanchoe plant is selected from the group consisting of Kalanchoe adelae, Kalanchoe arborescens, Kalanchoe beauverdii, Kalanchoe beharensis, Kalanchoe bentii, Kalanchoe blossfeldiana, Kalanchoe bouvetii, Kalanchoe bracteata, Kalanchoe campanulata, Kalanchoe crenata, Kalanchoe crundallii, Kalanchoe daigremontiana, Kalanchoe delagoensis, Kalanchoe dinklagei, Kalanchoe eriophylla, Kalanchoe farinacea, Kalanchoe fedtschenkoi, Kalanchoe figuereidoi, Kalanchoe flammea, Kalanchoe gastonis, Kalanchoe glaucescens, Kalanchoe gracilipes, Kalanchoe grandidieri, Kalanchoe grandiflora, Kalanchoe hildebrantii, Kalanchoe jongmansii, Kalanchoe kewensis, Kalanchoe laciniata, Kalanchoe laetivirens, Kalanchoe lateritia, Kalanchoe laxiflora, Kalanchoe linearifolia, Kalanchoe longiflora, Kalanchoe luciae, Kalanchoe macrochlamys, Kalanchoe manginii, Kalanchoe marnieriana, Kalanchoe marmorata, Kalanchoe millottii, Kalanchoe miniata, Kalanchoe nyikae, Kalanchoe obtusa, Kalanchoe orgyalis, Kalanchoe peltata, Kalanchoe petitiana, Kalanchoe pinnata, Kalanchoe porphyrocalyx, Kalanchoe prolifera, Kalanchoe pubescens, Kalanchoe pumila, Kalanchoe quartiniana, Kalanchoe rhombopilosa, Kalanchoe robusta, Kalanchoe rolandi, Kalanchoe rosei, Kalanchoe rotundifolia, Kalanchoe schizophylla, Kalanchoe serrata, Kalanchoe sexangularis, Kalanchoe streptantha, Kalanchoe suarezensis, Kalanchoe synsepala, Kalanchoe synsepala f. dissecta, Kalanchoe thyrsiflora, Kalanchoe tomentosa, Kalanchoe tubiflora, Kalanchoe uniflora, Kalanchoe velutina, Kalanchoe viguieri , and hybrids thereof.

4. The method according to claim 2 , wherein the resistance to mildew comprises resistance to the mildew pathogen Oidium kalanchoeae.

5. A Kalanchoe plant produced from the method according to claim 2 , wherein the plant comprises in its genome a nucleic acid sequence encoding a protein having the sequence of SEQ ID NO: 2 and exhibits resistance to mildew.

6. A seed, tissue, or plant part of the Kalanchoe plant according to claim 5 , wherein the seed, tissue, or plant part comprises in its genome a nucleic acid sequence encoding a protein that has the sequence of SEQ ID NO: 2.

7. A Kalanchoe plant comprising a protein comprising the sequence of SEQ ID NO: 2 and exhibiting resistance to mildew.

8. The Kalanchoe plant according to claim 7 , wherein the Kalanchoe plant is selected from the group consisting of Kalanchoe adelae, Kalanchoe arborescens, Kalanchoe beauverdii, Kalanchoe beharensis, Kalanchoe bentii, Kalanchoe blossfeldiana, Kalanchoe bouvetii, Kalanchoe bracteata, Kalanchoe campanulata, Kalanchoe crenata, Kalanchoe crundallii, Kalanchoe daigremontiana, Kalanchoe delagoensis, Kalanchoe dinklagei, Kalanchoe eriophylla, Kalanchoe farinacea, Kalanchoe fedtschenkoi, Kalanchoe figuereidoi, Kalanchoe flammea, Kalanchoe gastonis, Kalanchoe glaucescens, Kalanchoe gracilipes, Kalanchoe grandidieri, Kalanchoe grandiflora, Kalanchoe hildebrantii, Kalanchoe jongmansii, Kalanchoe kewensis, Kalanchoe laciniata, Kalanchoe laetivirens, Kalanchoe lateritia, Kalanchoe laxiflora, Kalanchoe linearifolia, Kalanchoe longiflora, Kalanchoe luciae, Kalanchoe macrochlamys, Kalanchoe manginii, Kalanchoe marnieriana, Kalanchoe marmorata, Kalanchoe millottii, Kalanchoe miniata, Kalanchoe nyikae, Kalanchoe obtusa, Kalanchoe orgyalis, Kalanchoe peltata, Kalanchoe petitiana, Kalanchoe pinnata, Kalanchoe porphyrocalyx, Kalanchoe prolifera, Kalanchoe pubescens, Kalanchoe pumila, Kalanchoe quartiniana, Kalanchoe rhombopilosa, Kalanchoe robusta, Kalanchoe rolandi, Kalanchoe rosei, Kalanchoe rotundifolia, Kalanchoe schizophylla, Kalanchoe serrata, Kalanchoe sexangularis, Kalanchoe streptantha, Kalanchoe suarezensis, Kalanchoe synsepala, Kalanchoe synsepala f. dissecta, Kalanchoe thyrsiflora, Kalanchoe tomentosa, Kalanchoe tubiflora, Kalanchoe uniflora, Kalanchoe velutina, Kalanchoe viguieri , and hybrids thereof.

9. A seed, tissue, or plant part of the Kalanchoe plant according to claim 7 , wherein the seed, tissue, or plant part comprises SEQ ID NO: 2.

Assignees

• Duemmen Group Bv

Inventors

• Vlielander Izaak Johannes
• Lang Chunting
• Maris Paulus Cornelis
• Peeters Roger Adrianus

Classifications

• A01H1/045

  using molecular markers · CPC title

• A01H6/324

  Kalanchoe · CPC title

• A01H5/02

  Flowers · CPC title

• A01H6/32

  Crassulaceae · CPC title

• C12N9/16 Primary

  acting on ester bonds (3.1) · CPC title