Papillomavirus pseudoviruses for detection and therapy of tumors

Patent metadata

Field	Value
Publication number	US-10814014-B2
Application number	US-201816235152-A
Country	US
Kind code	B2
Filing date	Dec 28, 2018
Priority date	May 8, 2007
Publication date	Oct 27, 2020
Grant date	Oct 27, 2020

Abstract

Disclosed herein are methods of detecting tumors, monitoring cancer therapy, and selectively inhibiting the proliferation and/or killing of cancer cells utilizing a papilloma pseudovirus or a papilloma virus-like particle (VLP).

First claim

What is claimed:

1. A method comprising administering to a melanoma in a subject a papilloma pseudovirus that comprises a fluorescent dye, and exposing the fluorescent dye in the melanoma in the subject to an excitation wavelength of light.

2. The method of claim 1 , wherein the fluorescent dye is chemically coupled to the papilloma pseudovirus.

3. The method of claim 2 , wherein the fluorescent dye is chemically coupled to the surface of the papilloma pseudovirus.

4. The method of claim 1 , wherein the papilloma pseudovirus is administered to the melanoma in an eye in the subject.

5. The method of claim 4 , wherein the papilloma pseudovirus is injected into the melanoma in an eye in the subject.

6. A method comprising administering to a melanoma in a subject a papilloma virus-like particle (VLP) that comprises a fluorescent dye, and exposing the fluorescent dye in the melanoma in the subject to an excitation wavelength of light.

7. The method of claim 6 , wherein the fluorescent dye is chemically coupled to the papilloma VLP.

8. The method of claim 7 , wherein the fluorescent dye is chemically coupled to the surface of the papilloma VLP.

9. The method of claim 6 , wherein the papilloma VLP is administered to the melanoma in an eye in the subject.

10. The method of claim 9 , wherein the papilloma VLP is injected into the melanoma in an eye in the subject.

11. A method for detecting the presence of melanoma cells bound to a papilloma pseudovirus or papilloma VLP comprising:
identifying a subject having or suspected of having melanoma cells;
administering to the subject a detectable amount of a papilloma pseudovirus or a papilloma VLP that comprises a fluorescent dye; and
detecting the presence of melanoma cells bound to the papilloma pseudovirus or papilloma VLP that comprises a fluorescent dye.

12. The method of claim 11 , wherein the fluorescent dye is chemically coupled to the papilloma pseudovirus or papilloma VLP.

13. The method of claim 12 , wherein the fluorescent dye is chemically coupled to the surface of the papilloma pseudovirus or papilloma VLP.

14. The method of claim 11 , wherein the detectable amount of the papilloma pseudovirus or papilloma VLP is administered to the melanoma in an eye in the subject.

15. The method of claim 14 , wherein the detectable amount of the papilloma pseudovirus or papilloma VLP is injected into the melanoma in an eye in the subject.

Assignees

• The Usa As Represented By The Sec Dep Of Health And Human Services
• The Usa As Represented By The Secretary Dept Of Health And Human Services

Inventors

• Roberts Jeffrey
• Lowy Douglas R
• Schiller John T

Classifications

• G01N33/575

  for cancer · CPC title

• A61K35/76 Primary

  Viruses; Subviral particles; Bacteriophages · CPC title

• A61P35/00

  Antineoplastic agents · CPC title

• A61K45/06

  Mixtures of active ingredients without chemical characterisation, e.g. antiphlogistics and cardiaca · CPC title

• A61K49/0485

  Nanoparticles, nanobeads, nanospheres, nanocapsules, i.e. having a size or diameter smaller than 1 micrometer · CPC title