Fungal endophytes for improved crop yields and protection from pests

Patent metadata

Field	Value
Publication number	US-10813359-B2
Application number	US-201415034862-A
Country	US
Kind code	B2
Filing date	Nov 6, 2014
Priority date	Nov 6, 2013
Publication date	Oct 27, 2020
Grant date	Oct 27, 2020

Abstract

The invention provides a synthetic combination of a crop and at least one fungal endophyte, wherein the crop is a host plant of the endophyte. Provided are also methods and compositions for producing such synthetic combinations. The endophyte reproduces and enhances the agronomic characteristics of the crop. Methods for inoculating the host plant with the endophyte, for propagating the host-endophyte combination, and for detecting the presence of the endophyte and of its metabolites within a host plant are also described.

First claim

The invention claimed is:

1. A method for increasing yield and/or reducing loss of a cotton plant, the method comprising:
contacting a surface of a cotton seed of the cotton plant with a spore suspension to produce a synthetic composition comprising the spore suspension coated onto the surface of the cotton seed, the spore suspension comprising:
purified filamentous, spore-forming, facultative fungal endophytes of species Cladosporium uredinicola or Epicoccum nigrum,
wherein the facultative fungal endophytes are present in the spore suspension in an amount effective to:
decrease the colonization frequencies of endophytes of genus Alternaria that are native to the cotton plant, and
provide a benefit to the cotton plant compared to a reference cotton plant grown from a seed untreated with the facultative fungal endophytes,
wherein the benefit is increased yield and/or reduced loss in the cotton plant.

2. The method of claim 1 , wherein the purified filamentous, spore-forming, facultative fungal endophytes are species Cladosporium uredinicola.

3. The method of claim 1 , wherein the purified filamentous, spore-forming, facultative fungal endophytes are species Epicoccum nigrum.

4. The method of claim 1 , wherein yield is increased by at least about 2%.

5. The method of claim 1 , wherein the spore suspension contains at least 100 (10{circumflex over ( )}2) spores/ml or 100,000 (10{circumflex over ( )}5) spores/g dry weight of the facultative fungal endophytes.

6. The method of claim 1 , wherein the cotton seed coating comprises at least one coating material in contact with the fungal endophytes.

7. The method of claim 6 , wherein the coating material is selected from alginic acid, carrageenan, dextrin, dextran, pelgel, polyethelene glycol, polyvinyl pyrrolidone, methyl cellulose, polyvinyl alcohol, gelatin, or combinations thereof.

8. The method of claim 1 , wherein the benefit further comprises increased pest-resistance, increased square retention, increased boll retention, increased biomass, increased root length, increased root mass, or enhanced resistance to drought stress.

9. The method of claim 8 , wherein the increased square retention is measured in the first fruiting position on branches as flowers begin to develop or the increased biomass is measured at mid-season in a field.

10. The method of claim 8 , wherein the enhanced resistance to drought stress is assessed by withholding water from 7-day old seedlings of the cotton plant grown in the greenhouse, wherein the seedlings have increased time to wilt or time to death as compared to a reference seedling grown from a reference seed.

11. A synthetic composition comprising of:
a combination of a cotton seed and Cladosporium uredinicola or Epicoccum nigrum , the combination produced by:
the method of contacting a surface of the cotton seed to produce the synthetic composition comprising a spore suspension coated onto the surface of the cotton seed, the spore suspension comprising:
purified filamentous, spore-forming facultative fungal endophytes of species Cladosporium uredinicola or Epicoccum nigrum,
wherein the facultative fungal endophytes are present in an amount effective to:
decrease the colonization frequencies of endophytes of genus Alternaria that are native to a cotton plant grown from the seed, and
provide a benefit to the cotton plant grown from the seed compared to a reference cotton plant grown from a seed untreated with the facultative fungal endophytes, wherein the benefit is an increased yield and/or reduced loss in the cotton plant.

12. The synthetic composition of claim 11 , wherein the purified filamentous, spore-forming, facultative fungal endophytes are species Cladosporium uredinicola.

13. The synthetic combination of claim 11 , wherein the purified filamentous, spore-forming, facultative fungal endophytes are species Epicoccum nigrum.

14. The synthetic composition of claim 11 , wherein yield is increased by at least about 2%.

15. The synthetic composition of claim 11 , wherein the spore suspension contains at least 100 (10{circumflex over ( )}2) spores/ml or 100,000 (10{circumflex over ( )}5) spores/g dry weight of the facultative fungal endophytes.

16. The synthetic composition of claim 11 , wherein the benefit further comprises increased pest-resistance, increased square retention, increased boll retention, increased biomass, increased root length, increased root mass, or enhanced resistance to drought stress.

17. The synthetic composition of claim 16 , wherein the increased square retention is measured in the first fruiting position on branches as flowers begin to develop or the increased biomass is measured at mid-season in a field.

18. The synthetic composition of claim 16 , wherein the enhanced resistance to drought stress is assessed by withholding water from 7-day old seedlings of the cotton plant grown in the greenhouse, wherein the seedlings have increased time to wilt or time to death as compared to a reference seedling grown from a reference seed.

Assignees

• Texas A & M Univ Sys

Inventors

• Sword Gregory A

Classifications

• A01N63/30 Primary

  Microbial fungi; Substances produced thereby or obtained therefrom · CPC title

• A01N65/00

  Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof (containing compounds of determined constitution A01N27/00 - A01N59/00) · CPC title

• A01H5/10

  Seeds · CPC title