Peptide tag and tagged protein including same

Patent metadata

Field	Value
Publication number	US-10808253-B2
Application number	US-201616066468-A
Country	US
Kind code	B2
Filing date	Dec 28, 2016
Priority date	Dec 28, 2015
Publication date	Oct 20, 2020
Grant date	Oct 20, 2020

Abstract

In this formula, X, Y, and Z each represent an amino acid residue independently selected from the group consisting of R, G, S, K, T, L, N, Q, and H, with the proviso that at least one Y represents K, L, N, Q, H, or R. m represents an integer of 0 to 5; n represents 1, 2, or 3; q represents an integer of 1 to 10; and r represents an integer of 0 to 10.

First claim

The invention claimed is:

1. A peptide comprising the amino acid sequence of SEQ ID NO: 25, 28, 30, 32, 34, 36, 38, 40, 42, 44, 46, 48, 50, 54, 64, 66, 102, 104, 106, 112, 114, 116, 118, 122, 124, 132, 136, 140, 142, 147, 149, 151, 153, or 157.

2. A tagged protein comprising:
the peptide according to claim 1 and
a protein.

3. The tagged protein according to claim 2 ,
wherein the protein is selected from the group consisting of human growth hormone, interferon β, xylanase, esterase, and green fluorescent protein (GFP).

4. The tagged protein according to claim 2 , wherein the peptide is linked to the protein through a protease recognition sequence.

5. The tagged protein according to claim 2 , further comprising:
a secretion signal.

6. A DNA encoding the tagged protein according to claim 2 .

7. A recombinant vector comprising:
the DNA according to claim 6 .

8. A transformant prepared by transformation with the DNA according to claim 6 .

9. The transformant according to claim 8 ,
wherein the transformant is yeast, E. coli, Brevibacillus , an insect cell, or a mammalian cell, and
wherein the mammalian cell comprises a human cultured cell, but does not comprise a human individual.

10. A method for producing a tagged protein, comprising:
culturing the transformant according to claim 8 to allow accumulation of the tagged protein, and
collecting the tagged protein.

11. A transformant prepared by transformation with the recombinant vector according to claim 7 .

12. A peptide consisting of the amino acid sequence of SEQ ID NO: 52, 58, 60, 92, 94, 96, 100, 108, 110, 120, 128, 130, 134, 145, or 155.

13. The peptide of claim 12 , wherein peptide consists of the amino acid sequence of SEQ ID NO: 52, 58, 60, 92, 94, 96, 100, 108, 110, 120, 130, 134, 145, or 155.

Assignees

• Idemitsu Kosan Co

Inventors

• Koike Kazuyoshi
• Takita Eiji
• Kaneda Koichi

Classifications

• C12Q2531/113

  PCR · CPC title

• C12Q1/6806

  Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay (C12Q1/6804 takes precedence) · CPC title

• C07K14/47

  from mammals · CPC title

• C07K14/43563

  from insects · CPC title

• C07K14/39

  from yeasts · CPC title