Nanopore biosensors for detection of proteins and nucleic acids

Patent metadata

Field	Value
Publication number	US-10802015-B2
Application number	US-201916521475-A
Country	US
Kind code	B2
Filing date	Jul 24, 2019
Priority date	Mar 25, 2013
Publication date	Oct 13, 2020
Grant date	Oct 13, 2020

Abstract

Described herein are nanopore biosensors based on a modified cytolysin protein. The nanopore biosensors accommodate macromolecules including proteins and nucleic acids, and may additionally comprise ligands with selective binding properties.

First claim

The invention claimed is:

1. A nanopore sensor system comprising:
i) a fluid-filled compartment separated by a membrane into a first cis chamber and a second trans chamber, wherein the fluid is an ionic solution;
ii) a ClyA pore inserted in the membrane, wherein the ClyA pore comprises 12 or more ClyA subunits and has a pore lumen of at least 3 nm in diameter, wherein the cis diameter of the pore lumen is at least 5.5 nm and the trans exit of the pore lumen is at least 3.3 nm,
wherein the each subunit of the ClyA pore comprises a polypeptide represented by an amino acid sequence with at least 80% identity to SEQ ID NO: 1; and
iii) electrodes configured for generating an electrical potential difference across the membrane to facilitate ionic flow through the ClyA pore from the first chamber to the second chamber.

2. The nanopore sensor system of claim 1 further comprising a ligand in combination with the ClyA pore.

3. The nanopore sensor according to claim 2 , wherein the ligand confers selective binding properties to an analyte present in the ionic solution.

4. The nanopore sensor according to claim 3 , wherein the ligand is an aptamer.

5. The nanopore sensor according to claim 3 , wherein the ligand is selected to bind to a specific target protein analyte.

6. The nanopore sensor according to claim 3 , wherein the ligand is an antibody.

7. The nanopore sensor according to claim 3 , wherein the ligand is a receptor.

8. The nanopore sensor according to claim 3 , wherein the ligand is a peptide.

9. The nanopore sensor according to claim 5 , wherein the protein analyte binds within the lumen of the ClyA pore.

10. The nanopore sensor according to claim 5 , wherein the protein analyte is a protein with a molecular weight in the range of 15-70 kDa.

11. The nanopore sensor according to claim 9 , wherein the protein analyte is a protein with a molecular weight in the range of 15-70 kDa.

12. The nanopore sensor according to claim 1 , wherein the lumen of the ClyA pore is modified to alter the size, binding properties, and/or structure of the pore.

13. The nanopore sensor according to claim 2 , wherein the ClyA pore is attached to the ligand.

14. The nanopore sensor according to claim 13 , wherein the ClyA pore is attached to the ligand via a disulfide linkage.

15. The nanopore sensor according to claim 13 , wherein the ClyA pore is attached to the ligand via cross-linking.

16. The nanopore sensor according to claim 13 , wherein the ClyA pore is attached to the ligand via chemical ligation.

17. The nanopore sensor according to claim 2 , wherein the ClyA pore is not attached to the ligand.

Assignees

• Univ Leuven Kath

Inventors

• Maglia Giovanni
• Soskine Mikhael

Classifications

• C07K14/255 Primary

  Salmonella (G) · CPC title

• C12Q1/6869

  Methods for sequencing · CPC title

• C12Q1/68

  involving nucleic acids · CPC title

• G01N27/3278

  involving nanosized elements, e.g. nanogaps or nanoparticles (nanopores G01N33/48721; magnetic beads G01N27/745) · CPC title

• G01N33/54306 Primary

  Solid-phase reaction mechanisms · CPC title