Chromatography column assembly

Patent metadata

Field	Value
Publication number	US-10802002-B2
Application number	US-201815990097-A
Country	US
Kind code	B2
Filing date	May 25, 2018
Priority date	Aug 26, 2011
Publication date	Oct 13, 2020
Grant date	Oct 13, 2020

Abstract

Described is a chromatographic column assembly that includes an outer tube comprising a metal, a first conduit disposed within the outer tube, a second conduit disposed within the outer tube, and a first joint located between the first conduit and the second conduit. The outer tube is deformed by a first uniform radial crimp at a longitudinal location along the outer tube that surrounds the first conduit on a first side of the first joint, and a second uniform radial cramp at a longitudinal location along the outer tube that surrounds the second conduit on a second side of the first joint. The first and second uniform radial cramps form a fluid-tight seal between the first conduit and the second conduit and each have a substantially flat base region over which a diameter of the outer tube is reduced for a non-zero longitudinal length.

First claim

What is claimed is:

1. A chromatographic column assembly, comprising:
an outer tube comprising a metal;
an intermediate tube comprising a polymeric material and disposed within the outer tube;
a sorbent bed tube disposed within the intermediate tube and having a first end and a second end opposite the first end;
a sorbent bed disposed within the sorbent bed tube; and
a first inner tube disposed within the intermediate tube and having a first inner tube end abutting the first end of the sorbent bed tube, thereby defining a first joint;
wherein the outer tube is deformed by a first uniform radial crimp at a longitudinal location along the outer tube that surrounds the first inner tube on a first side of the first joint,
wherein the outer tube is deformed by a second uniform radial crimp at a longitudinal location along the outer tube that surrounds the sorbent bed tube on a second side of the first joint, and
wherein the first and second uniform radial cramps form a fluid-tight seal between the first inner tube end and the first end of the sorbent bed tube, wherein the first and second uniform radial crimps each have a flat base region over which a diameter of the outer tube is reduced in a controlled manner over a longitudinal length, wherein the longitudinal length of the flat base regions of the first and second uniform radial crimps is each at least one millimeter.

2. The chromatographic column assembly of claim 1 , further comprising:
a second inner tube disposed within the intermediate tube and having a second inner tube end abutting the second end of the sorbent bed tube, thereby defining a second joint,
wherein the outer tube is deformed by a third uniform radial crimp at a longitudinal location along the outer tube that surrounds the second inner tube on a first side of the second joint,
wherein the outer tube is deformed by a fourth uniform radial crimp at a longitudinal location along the outer tube that surrounds the sorbent bed tube on a second side of the second joint,
wherein the third and fourth uniform radial cramps form a fluid-tight seal between the second inner tube end and the second end of the sorbent bed tube.

3. The chromatographic column assembly of claim 1 , further comprising a pair of frits, one of the frits disposed at an end of the sorbent bed and the other of the frits disposed at an opposite end of the sorbent bed.

4. The chromatographic column assembly of claim 3 , wherein each of the frits is disposed within the sorbent bed tube and at an end of the first inner tube.

5. The chromatographic column assembly of claim 3 , wherein one of the fits is disposed within the first inner tube at the first inner tube end and the other of the frits is disposed within the second inner tube at the second inner tube end.

6. The chromatographic column assembly of claim 1 , wherein an inner diameter of the sorbent bed tube is different from an inner diameter of the first inner tube.

7. The chromatographic column assembly of claim 1 , wherein an outer diameter of the sorbent bed tube is different from an outer diameter of the first inner tube.

8. The chromatographic column assembly of claim 1 , wherein the sorbent bed tube comprises fused silica.

9. The chromatographic column assembly of claim 8 , wherein the sorbent bed tube and the intermediate tube are formed as an integrated structure.

10. The chromatographic column assembly of claim 1 , wherein the sorbent bed tube comprises stainless steel.

11. The chromatographic column assembly of claim 10 , wherein the sorbent bed tube and the intermediate tube are formed as an integrated structure.

12. The chromatographic column assembly of claim 1 wherein the intermediate tube includes three intermediate tubes, a first intermediate tube having a length that is the same as a length of the sorbent bed tube, and a second and third intermediate tube disposed within the outer tube and having an end abutting an end of the first intermediate tube.

13. The chromatographic column assembly of claim 1 , further comprising a ferrule disposed on the outer tube proximate to one end of the outer tube for engaging a coupling body at a fluid port.

14. The chromatographic column assembly of claim 1 , wherein the first and second uniform radial crimps each have a transition region on each side of the substantially flat base region that transitions between the reduced diameter of the substantially flat base region and an uncrimped outer diameter of the outer tube.

15. The chromatographic column assembly of claim 1 , wherein the first and second uniform radial crimps are each formed by a collet having a machined circular bore.

16. A chromatographic column assembly, comprising:
an outer tube comprising a metal;
a first conduit disposed within the outer tube;
a second conduit disposed within the outer tube; and
a first joint located between the first conduit and the second conduit;
wherein the outer tube is deformed by a first uniform radial crimp at a longitudinal location along the outer tube that surrounds the first conduit on a first side of the first joint,
wherein the outer tube is deformed by a second uniform radial crimp at a longitudinal location along the outer tube that surrounds the second conduit on a second side of the first joint,
wherein the first and second uniform radial crimps form a fluid-tight seal between the first conduit and the second conduit, and
wherein the first and second uniform radial crimps each have a flat base region over which a diameter of the outer tube is reduced in a controlled manner over a longitudinal length, wherein the longitudinal length of the flat base regions of the first and second uniform radial crimps is each greater than 6 millimeters.

17. The chromatographic column assembly of claim 16 , wherein the first and second uniform radial crimps each have a transition region on each side of the substantially flat base region that transitions between the reduced diameter of the substantially flat base region and an uncrimped outer diameter of the outer tube.

18. The chromatographic column assembly of claim 16 , wherein the first and second uniform radial crimps are each formed by a collet having a machined circular bore.

Assignees

• Waters Technologies Corp

Inventors

• Michienzi Joseph D
• Fadgen Keith
• Leveille Wade P
• Fisk Raymond P
• Marszalkowski Jr Frank John

Classifications

• G01N30/7266

  by electric field, e.g. electrospray · CPC title

• G01N30/60

  Construction of the column · CPC title

• G01N30/6004

  end pieces · CPC title

• B01D15/22 Primary

  relating to the construction of the column · CPC title

• G01N30/6052

  body · CPC title