Method and device for raman spectroscopy

Patent metadata

Field	Value
Publication number	US-10794766-B2
Application number	US-201716313668-A
Country	US
Kind code	B2
Filing date	Jun 23, 2017
Priority date	Jun 27, 2016
Publication date	Oct 6, 2020
Grant date	Oct 6, 2020

Abstract

The method includes irradiation of excitation radiation onto a sample to be examined. The sample is irradiated with a first excitation radiation of a first excitation wavelength and a different second excitation radiation of a second excitation wavelength. The first excitation radiation scattered by the sample is wavelength-selective filtered by means of a passive filter element. A transmitted filter wavelength of the filter element differs from at least the first excitation wavelength and the second excitation wavelength. A first intensity is determined through a single-channel detector assigned to the filter wavelength from the first excitation radiation scattered and filtered by the sample. Additionally, the filter element wavelength-selective filters the second excitation radiation scattered by the sample. A second intensity is determined through the single-channel detector assigned to the filter wavelength from the second excitation radiation scattered and filtered by the sample.

First claim

The invention claimed is:

1. A method for recording a Raman spectrum, comprising the following method steps:
irradiating of excitation radiation onto a sample to be examined, whereby the sample to be examined is irradiated with a first excitation radiation of a first excitation wavelength and a second excitation radiation of a second excitation wavelength, whereby at least the first excitation wavelength differs from the second excitation wavelength,
wavelength-selective filtering of the first excitation radiation scattered by the sample by a passive filter element, whereby a transmitted filter wavelength of the filter element differs from at least the first excitation wavelength and the second excitation wavelength, and whereby through a single-channel detector assigned to the filter wavelength from the first excitation radiation scattered and filtered by the sample, a first intensity is determined,
wavelength-selective filtering of the second excitation radiation scattered by the sample by the filter element, whereby through the single-channel detector assigned to the filter wavelength from the second excitation radiation scattered and filtered by the sample, a second intensity is determined, whereby at least two determined intensities are stored,
wavelength-selective filtering at least of the first excitation radiation scattered by the sample and the second excitation radiation scattered by the sample by at least one further passive filter element, whereby the transmitted filter wavelengths of the individual filter elements differ both from each other and also respectively from the individual excitation wavelengths of the respective excitation radiations, whereby through one each of the further single-channel detectors assigned to the at least one further filter wavelength, at least one first intensity and one second intensity are respectively determined from the excitation radiation scattered by the sample and filtered, whereby at least two determined intensities are stored for the at least one further filter wavelength,
whereby from at least four determined intensities with at least two filter wavelengths, conclusions are drawn about the sample, and
wherein the spectral distance between at least one excitation radiation and a scattered and filtered excitation radiation corresponds to precisely one Raman displacement of the excitation radiation scattered by the sample.

2. The method of claim 1 , wherein the transmitted filter areas around the filter wavelengths of the respective filter elements spectrally overlap at least partially for at least two of these filter areas.

3. The method of claim 1 , wherein the excitation radiation sources are narrow band, continuously spectrally adjustable over a wide range and directly frequency modulated laser diodes.

4. The method of claim 1 , wherein the excitation radiation sources are dual wavelength diode lasers, in particular Y-branched dual wavelength DBR diode lasers.

5. The method of claim 1 , wherein passive filter elements are narrow-band bandpass filters, whereby a filter wavelength is determined by the central wavelength of such a bandpass.

6. The method of claim 1 , wherein the excitation radiation sources are dual wavelength diode lasers, in particular Y-branched dual wavelength DBR diode lasers.

7. A Raman spectroscopy device comprising:
at least one excitation source, designed to irradiate excitation radiation onto a sample to be examined, and to radiate the sample with at least one first excitation radiation of a first excitation wavelength and a second excitation radiation of a first excitation wavelength, whereby at least the first excitation wavelength differs from the second excitation wavelength,
a passive filter element designed to wavelength-selectively filter at least the first excitation radiation scattered by the sample and the second excitation radiation scattered by the sample, whereby a filter wavelength of the passive filter element differs from at least the first excitation wavelength and the second excitation wavelength,
a single-channel detector, assigned to the passive filter element, designed to determine at least one first intensity of the first excitation radiation scattered by the sample and filtered and a second intensity of the second excitation radiation scattered by the sample and filtered, whereby at least two determined intensities are stored,
at least one further passive filter element, whereby the transmitted filter wavelengths of the individual filter elements differ both from each other and respectively from the individual excitation wavelengths of the respective excitation radiations; and
one of the at least one further single-channel detectors assigned to the at least one further filter wavelength, designed to determine at least one first intensity and one second intensity respectively from the excitation radiation scattered by the sample and filtered, whereby the at least two intensities for the at least one further filter wavelength are stored,
wherein a means for evaluation is designed to draw conclusions about the sample from at least four determined intensities with at least two filter wavelengths,
wherein the spectral distance between at least one excitation radiation and a scattered and filtered excitation radiation corresponds to precisely one Raman displacement of the excitation radiation scattered by the sample.

8. The device according to claim 7 , further comprising:
first means for coupling designed to couple the excitation radiation emitted from the excitation source into the sample to be examined,
second means for coupling designed to couple the excitation radiation scattered by the sample into a single-channel detector, whereby the filter element is arranged such that the excitation radiation scattered by the sample passes the filter element before reaching the single-channel detector.

9. The device according to claim 7 , wherein the excitation sources are spectrally adjustable and direct frequency-modulated laser diodes.

10. The device according to claim 7 , wherein the excitation radiation sources are dual wavelength diode lasers, in particular Y-branched dual wavelength DBR diode lasers.

11. The device according to claim 7 , wherein the transmitted filter areas around the filter wavelengths of the respective filter elements at least partially spectrally overlap for at least two of these filter areas.

Assignees

• Forschungsverbund Berlin Ev

Inventors

• Sumpf Bernd
• Maiwald Martin

Classifications

• G01J3/36

  Investigating two or more bands of a spectrum by separate detectors · CPC title

• G01J3/44 Primary

  Raman spectrometry; Scattering spectrometry {; Fluorescence spectrometry} · CPC title

• G01J3/28

  Investigating the spectrum (using colour filters G01J3/51) · CPC title

• G01J2003/1213

  Filters in general, e.g. dichroic, band · CPC title

• G01N21/65 Primary

  Raman scattering · CPC title