Lactic acid bacteria compositions

Patent metadata

Field	Value
Publication number	US-10792316-B2
Application number	US-201615092473-A
Country	US
Kind code	B2
Filing date	Apr 6, 2016
Priority date	Jun 30, 2011
Publication date	Oct 6, 2020
Grant date	Oct 6, 2020

Abstract

The invention relates to a dry compositions for lactic acid bacteria and in particular to a dry composition comprising from 109 to 1013 cfu/g of the composition of lactic acid bacteria cells, wherein the composition is characterized by that it also comprises following amounts of protective agents (all amounts of protective agents below are given relative to 1 g of lactic acid bacteria cells in the composition): from 6 to 9 g of trehalose, from 0.1 to 1 g of inulin and from 0.5 to 3 g of hydrolyzed casein, and by that it does not comprise a salt of alginic acid. The composition has an improved storage stability of the cell of interest. Comparison experiments have been made between compositions with and without alginate and it has been found that there is substantially no difference between compositions with or without alginate with regard to stability. Further, the invention relates to a method for preparing a dry lactic acid bacteria composition.

First claim

The invention claimed is:

1. A dry composition comprising lactic acid bacteria cells in an amount from 10 9 to 10 13 cfu/g of the composition, and further comprising the following amounts of protective agents per g of lactic acid bacteria cells in the composition:
from 6 to 9 g trehalose,
from 0.1 to 1 g inulin and
from 0.5 to 3 g hydrolyzed casein,
wherein the composition does not comprise a salt of alginic acid, and

wherein the log loss of active cells in the composition after 13 weeks is less than 2.5 when stored at 30% relative humidity (RH) and 35° C.

2. A dry composition according to claim 1 , which comprises 75-80% (w/w) trehalose, 3-10% (w/w) inulin and 15-20% (w/w) hydrolysed casein.

3. A dry composition according to claim 1 , wherein the lactic acid bacteria cells are from at least one lactic acid bacteria species selected from the group consisting of Lactococcus species, Streptococcus species, Enterococcus species, Lactobacillus species, Leuconostoc species, Bifidobacterium species, Propioni and Pediococcus species.

4. A dry composition according to claim 3 , wherein the lactic acid bacteria cells are from at least one lactic acid bacteria species selected from the group consisting of Lactobacillus rhamnosus, Lactococcus lactis subsp. lactis, Lactococcus lactis subsp. cremoris, Leuconostoc lactis, Leuconostoc mesenteroides subsp. cremoris, Pediococcus pentosaceus, Lactococcus lactis subsp. lactis biovar. diacetylactis, Lactobacillus casei subsp. casei, Streptococcus thermophilus, Enterococcus, Bifidobacterium animalis, Bifidobacterium lactis, Bifidobacterium longum, Lactobacillus lactis, Lactobacillus helveticus, Lactobacillus fermentum, Lactobacillus salivarius, Lactobacillus delbrueckii subsp. bulgaricus and Lactobacillus acidophilus.

5. A dry composition according to claim 4 , wherein the lactic acid bacteria cells are from at least one lactic acid bacteria strain selected from the group consisting of Bifidobacterium animalis subsp lactis deposited as DSM 15954, Bifidobacterium animalis subsp lactis deposited as ATCC 27536, Bifidobacterium animalis subsp lactis deposited as DSM 10140, Lactobacillus acidophilus deposited as DSM 13241, Lactobacillus rhamnosus deposited as ATCC 53103, Lactobacillus rhamnosus deposited as ATCC 55826, Lactobacillus reuteri deposited as ATCC 55845, Lactobacillus paracasei subsp. paracasei deposited as ATCC 55544, Lactobacillus paracasei deposited as LMG-17806, Streptococcus thermophilus deposited as DSM 15957, Lactobacillus fermentum deposited as NM02/31074, and Lactobacillus paracasei subsp. paracasei deposited as CCTCC M204012.

6. The dry composition according to claim 1 , wherein the weight of the dry composition is from 50 g to 1000 kg.

7. The dry composition according to claim 1 , wherein the weight of the dry composition is from 100 g to 1000 kg.

8. The dry composition according to claim 1 , wherein the weight of the dry composition is from 1 kg to 1000 kg.

9. An infant powder comprising a composition according to claim 1 .

10. A food product comprising a composition according to claim 1 .

11. A dietary supplement comprising a composition according to claim 1 .

12. A method for preparing a dry composition according to claim 1 , comprising:
(a) fermenting lactic acid bacteria cells and harvesting the cells to obtain a lactic acid bacteria cell concentrate comprising the lactic acid bacteria cells and water, wherein the concentrate comprises from 10 8 to 10 14 cfu lactic acid bacteria cells per g dry matter of the concentrate;
(b) forming a slurry by mixing the lactic acid bacteria cell concentrate with the following amounts of protective agents per g of lactic acid bacteria cells in the slurry:
from 6 to 9 g trehalose,
from 0.1 to 1 g inulin and
from 0.5 to 3 g hydrolyzed casein,
wherein the amounts of the lactic acid bacteria cells and protective agents are measured as dry matter in the slurry, and wherein the slurry does not comprise a salt of alginic acid;
(c) freezing the slurry to form solid frozen particles/pellets;
(d) loading a tray with from 2 kg/m 2 to 50 kg/m 2 of the frozen particles/pellets to obtain material comprising lactic acid bacteria cells;
(e) primary drying the material under a vacuum pressure of from 0.7 to 2 millibar at a temperature such that the temperature of the material does not get so high that more than 75% of the lactic acid bacteria cells are inactivated and for a period of time until at least 90% of the water from the slurry of step (b) has been removed; and
(f) secondary drying the material of step (e) under a vacuum pressure of from 0.01 to 0.6 millibar at a temperature such that the temperature of the material does not get so high that more than 75% of the lactic acid bacteria cells are inactivated and for a period of time sufficient to reduce the water activity (a w ) to less than 0.30,

thereby obtaining the dry composition comprising lactic acid bacteria cells in an amount from 10 9 to 10 13 cfu/g of the composition, wherein the composition further comprises the following amounts of protective agents per g of lactic acid bacteria cells in the composition:
from 6 to 9 g trehalose,
from 0.1 to 1 g inulin and
from 0.5 to 3 g hydrolyzed casein,
and wherein the composition does not comprise a salt of alginic acid.

13. The method of claim 12 wherein the temperature of the material in step (e) does not get so high that more than 10% of the lactic acid bacteria cells are inactivated; and the temperature of the material in step (f) does not get so high that more than 10% of the lactic acid bacteria cells are inactivated.

14. The method of claim 12 , wherein the composition comprises 75-80% (w/w) trehalose, 3-10% (w/w) inulin and 15-20% (w/w) hydrolysed casein.

15. The composition of claim 1 , made by a process comprising:
(a) forming a slurry of a concentrate comprising the lactic acid bacteria cells and water, wherein the concentrate comprises from 10 8 to 10 14 cfu lactic acid bacteria cells per g dry matter of the concentrate, with from 6 to 9 g trehalose, from 0.1 to 1 g inulin, and from 0.5 to 3 g hydrolyzed casein, wherein the amounts of the lactic acid bacteria cells and protective agents are measured as dry matter in the slurry, and wherein the slurry does not comprise a salt of alginic acid;
(b) freezing the slurry to form solid frozen particles/pellets;
(c) primary drying the solid frozen particles/pellets material under a vacuum pressure of from 0.7 to 2 millibar at a temperature such that the temperature of the material does not get so high that more than 75% of the lactic acid bacteria cells are inactivated and for a period of time until at least 90% of the water from the slurry has been removed; and
(d) secondary drying the material of step (c) under a vacuum pressure of from 0.01 to 0.6 millibar at a temperature such that the temperature of the material does not get so high that more than 75% of the lactic acid bacteria cells are inactivated and for a period of time sufficient to reduce the water activity (a w ) to less than 0.30.

Assignees

• Chr Hansen As

Inventors

• Yde Birgitte
• Svendsen Jakob Blenker

Classifications

• C12N1/04 Primary

  Preserving or maintaining viable microorganisms (immobilised microorganisms C12N11/00) · CPC title

• A61K47/22

  Heterocyclic compounds, e.g. ascorbic acid, tocopherol or pyrrolidones · CPC title

• A61K35/747 Primary

  Lactobacilli, e.g. L. acidophilus or L. brevis · CPC title

• A23L29/065

  Microorganisms (addition of bacteria for nutritional purposes A23L33/135) · CPC title

• A23L33/22

  Comminuted fibrous parts of plants, e.g. bagasse or pulp · CPC title