Graphene oxide-based nanolab and methods of detecting of exosomes

Patent metadata

Field	Value
Publication number	US-10788486-B2
Application number	US-201715728155-A
Country	US
Kind code	B2
Filing date	Oct 9, 2017
Priority date	Oct 9, 2016
Publication date	Sep 29, 2020
Grant date	Sep 29, 2020

Abstract

A capture device for capturing a biological substance can include: a substrate; a graphene-oxide layer on the substrate; at least one polydopamine polymer coupled with the graphene-oxide; and at least one targeting receptor coupled to the polydopamine(s), wherein the targeting receptor is capable of targeting/binding with a target biological substance. The graphene-oxide may be covalently coupled with the substrate and polydopamine, and the polydopamine may be covalently coupled with the targeting receptor. The targeting receptor can be an antibody or fragment thereof. The target biological substance can be an exosome. The substrate can be a particle (e.g., magnetic, such as magnetically responsive) or a surface in a microfluidic channel. The surface can be a top surface of a post, the post having a Y-shaped cross-sectional profile. In one aspect, the substrate is a particle. The capture device can include the target biological substance bound to the targeting receptor.

First claim

The invention claimed is:

1. A graphene-oxide coated particle for capturing a biological substance, the particle comprising:
a core particle having a surface;
a graphene-oxide-layer coating said core particle, said graphene-oxide layer comprising graphene-oxide nanosheets, said graphene-oxide nanosheets being covalently bonded to the particle surface;
at least one polydopamine polymer coupled with the graphene-oxide nanosheets; and
at least one targeting receptor coupled to the at least one polydopamine polymer, wherein the targeting receptor is capable of targeting and binding with a target biological sub stance.

2. The graphene-oxide coated particle of claim 1 , wherein the core particle includes a functional group covalently coupled with a functional group of the graphene-oxide layer to form the covalent bond between the surface and graphene-oxide nanosheets.

3. The graphene-oxide coated particle of claim 1 , wherein the graphene-oxide nanosheets include a functional group that is covalently coupled with a functional group of the at least one polydopamine polymer to form a covalent bond between the graphene-oxide nanosheets and polydopamine polymer.

4. The graphene-oxide coated particle of claim 1 , wherein the at least one polydopamine polymer includes a functional group that is covalently coupled with a functional group of the at least one targeting receptor to form a covalent bond between the polydopamine polymer and targeting receptor.

5. The graphene-oxide coated particle of claim 1 , wherein the targeting receptor is an antibody or fragment thereof.

6. The graphene-oxide coated particle of claim 1 , wherein the target biological substance is an exosome.

7. The graphene-oxide coated particle of claim 1 , wherein the core particle is a core/shell magnetic particle having a magnetic core and a functionalized shell.

8. The graphene-oxide coated particle of claim 1 , wherein
the graphene-oxide nanosheets are covalently bonded to the at least one polydopamine polymer; and wherein
the at least one polydopamine polymer is covalently bonded to the at least one targeting receptor.

9. The graphene-oxide coated particle of claim 1 , comprising the target biological substance bound to the targeting receptor.

10. The graphene-oxide coated particle of claim 1 , wherein said particle is a magnetic bead.

11. The graphene-oxide coated particle of claim 1 , wherein said graphene-oxide layer comprises a plurality of said graphene-oxide nanosheets covalently bonded to said substrate surface, said graphene-oxide nanosheets having a plurality of polydopamine polymers coupled thereto.

12. The graphene-oxide coated particle of claim 11 , wherein each polydopamine polymer extends from the graphene-oxide layer away from the particle.

13. The graphene-oxide coated particle of claim 11 , wherein said plurality of polydopamine polymers form a layer having a 3-dimensional, porous morphology on said graphene-oxide layer.

14. A method of capturing a target biological substance, the method comprising:
providing the graphene-oxide coated particle of claim 1 ; and
contacting a sample with the graphene-oxide coated particle such that the target biological substance associates with the targeting receptor.

15. The method of claim 14 , detecting the presence of the target biological substance being bound with the targeting receptor.

16. The method of claim 14 , comprising one of:
removing the graphene-oxide coated particle from the sample; or
removing the sample from the graphene-oxide coated particle.

17. The method of claim 14 , comprising dissociating the target biological substance from the targeting receptor.

18. The method of claim 15 , comprising qualitatively or quantitatively determining an amount or relative amount of the target biological substance in the sample.

19. A method of determining a disease state in a subject, the method comprising:
providing the graphene-oxide coated particle of claim 1 ;
obtaining a sample from the subject;
contacting the sample with the graphene-oxide coated particle;
and
detecting the presence of the target biological substance in the sample, wherein if the target biological substance binds with the targeting receptor, the target biological substance is present in the sample, or if the target biological substance does not bind with the targeting receptor, the target biological substance is absent in the sample to thereby determine the disease state in the subject.

Assignees

• Univ Kansas
• Univ Kansas State

Inventors

• Zhang Peng
• Zeng Yong
• He Mei

Classifications

• B01L2200/0631

  Purification arrangements, e.g. solid phase extraction [SPE] · CPC title

• B01L2300/0636

  Integrated biosensor, microarrays · CPC title

• B01L2300/0816

  Cards, e.g. flat sample carriers usually with flow in two horizontal directions · CPC title

• B01L3/502707

  characterised by the manufacture of the container or its components · CPC title

• B01L2200/0647

  Handling flowable solids, e.g. microscopic beads, cells, particles · CPC title