Pesticidal genes and methods of use

Patent metadata

Field	Value
Publication number	US-10787679-B2
Application number	US-201715697183-A
Country	US
Kind code	B2
Filing date	Sep 6, 2017
Priority date	Sep 6, 2016
Publication date	Sep 29, 2020
Grant date	Sep 29, 2020

Abstract

Compositions having pesticidal activity and methods for their use are provided. Compositions include isolated and recombinant polypeptides having pesticidal activity, recombinant and synthetic nucleic acid molecules encoding the polypeptides, DNA constructs and vectors comprising the nucleic acid molecules, host cells comprising the vectors, and antibodies to the polypeptides. Nucleotide sequences encoding the polypeptides can be used in DNA constructs or expression cassettes for transformation and expression in organisms of interest. The compositions and methods provided are useful for producing organisms with enhanced pest resistance or tolerance. Transgenic plants and seeds comprising a nucleotide sequence that encodes a pesticidal protein of the invention are also provided. Such plants are resistant to insects and other pests. Methods are provided for producing the various polypeptides disclosed herein, and for using those polypeptides for controlling or killing a pest. Methods and kits for detecting polypeptides of the invention in a sample are also included.

First claim

That which is claimed is:

1. A recombinant nucleic acid molecule encoding a pesticidal polypeptide comprising at least 95% sequence identity to the amino acid sequence set forth in SEQ ID NOs: 185 or 186;
wherein said recombinant nucleic acid molecule is not a naturally occurring sequence encoding said polypeptide.

2. The recombinant nucleic acid molecule of claim 1 , wherein said recombinant nucleic acid molecule is a synthetic sequence designed for expression in a plant.

3. The recombinant nucleic acid molecule of claim 1 , wherein said recombinant nucleic acid molecule is operably linked to a promoter capable of directing expression in a plant cell.

4. The recombinant nucleic acid molecule of claim 1 , wherein said recombinant nucleic acid molecule is operably linked to a promoter capable of directing expression in a bacteria.

5. A host cell comprising the recombinant nucleic acid molecule of claim 1 .

6. The host cell of claim 5 , wherein said host cell is a bacterial host cell.

7. A DNA construct comprising a promoter that drives expression in a plant cell operably linked to a recombinant nucleic acid molecule comprising a nucleotide sequence that encodes a polypeptide comprising an amino acid sequence having at least 95% sequence identity to the amino acid sequence set forth in SEQ ID NOs: 185 or 186.

8. The DNA construct of claim 7 , wherein said nucleotide sequence is a synthetic DNA sequence designed for expression in a plant.

9. A vector comprising the DNA construct of claim 7 .

10. A host cell comprising the vector of claim 9 .

11. A composition comprising the host cell of claim 10 .

12. The composition of claim 11 , wherein said composition is selected from the group consisting of a powder, dust, pellet, granule, spray, emulsion, colloid, and solution.

13. The composition of claim 12 , wherein said composition comprises from about 1% to about 99% by weight of said polypeptide.

14. A method for producing a polypeptide with pesticidal activity comprising culturing the host cell of claim 10 under conditions in which the nucleic acid molecule encoding the polypeptide is expressed.

15. A plant having stably incorporated into its genome a DNA construct comprising a nucleotide sequence that encodes a protein having pesticidal activity, wherein said nucleotide sequence encodes a polypeptide comprising an amino acid sequence having at least 95% sequence identity to the amino acid sequence set forth in SEQ ID NOs: 185 or 186.

16. A transgenic seed of the plant of claim 15 .

17. A method for protecting a plant from an insect pest, comprising expressing in a plant or cell thereof a nucleotide sequence that encodes a pesticidal polypeptide, wherein said nucleotide sequence encodes a polypeptide comprising an amino acid sequence having at least 95% sequence identity to the amino acid sequence set forth in SEQ ID NOs: 185 or 186.

18. The method of claim 17 , wherein said plant produces a pesticidal polypeptide having pesticidal activity against at least one of a lepidopteran pest, a coleopteran pest, or a hemipteran pest.

19. A method for increasing yield in a plant comprising growing in a field a plant or seed thereof having stably incorporated into its genome a DNA construct comprising a promoter that drives expression in a plant operably linked to a nucleic acid molecule that encodes a pesticidal polypeptide, wherein said nucleic acid molecule comprises a nucleotide sequence that encodes a polypeptide comprising an amino acid sequence having at least 95% sequence identity to the amino acid sequence set forth in SEQ ID NOs: 185 or 186.

20. A method for controlling a pest population comprising contacting said pest population with a pesticidal-effective amount of the polypeptide produced by the method of 18.

21. The recombinant nucleic acid molecule of claim 1 , wherein the recombinant nucleic acid molecule encodes a polypeptide comprising the amino acid sequence set forth in SEQ ID NOs: 185 or 186.

22. The DNA construct of claim 7 , wherein the nucleotide sequence encodes a polypeptide comprising the amino acid sequence set forth in SEQ ID NOs: 185 or 186.

23. The plant of claim 15 , wherein the nucleotide sequence encodes a polypeptide comprising the amino acid sequence set forth in SEQ ID NOs: 185 or 186.

24. The method of claim 17 , wherein the nucleotide sequence encodes a polypeptide comprising the amino acid sequence set forth in SEQ ID NOs: 185 or 186.

25. The method of claim 19 , wherein the nucleic acid molecule comprises a nucleotide sequence that encodes a polypeptide comprising the amino acid sequence of any one of SEQ ID NOs: 185 or 186.

Assignees

• Agbiome Inc

Inventors

• Parks Jessica
• Roberts Kira Bulazel
• Thayer Rebecca E

Classifications

• C12N15/8286 Primary

  for insect resistance · CPC title

• C12N15/8285

  for nematode resistance · CPC title

• C12N15/74

  Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora · CPC title

• C07K14/36

  from Actinomyces; from Streptomyces (G) · CPC title

• C07K14/32 Primary

  from Bacillus (G) · CPC title