Micro-channel imaging system for living organism

Patent metadata

Field	Value
Publication number	US-10786341-B2
Application number	US-201715829923-A
Country	US
Kind code	B2
Filing date	Dec 3, 2017
Priority date	Oct 25, 2017
Publication date	Sep 29, 2020
Grant date	Sep 29, 2020

Abstract

An imaging system for a living organism is described and has a receiving device used to receive the living organism and formed with at least two receiving grooves, at least one micro-channel connected to the receiving grooves, and a plurality of magnetic cilia disposed on a surface of the micro-channel; a magnetic field providing device neighboring a first side of the receiving device to control an action of the magnetic cilia; a driving device neighboring a second side of the receiving device to drive a movement of the living organism and control a moving direction thereof; a liquid providing device in communication with the receiving device to provide liquid; and an image capturing device neighboring a third side of the receiving device to capture an image of the living organism.

First claim

The invention claimed is:

1. An imaging system, suitable for a living organism, comprising:
a receiving device configured to receive the living organism, the receiving device comprising:
at least one micro-channel; and
a plurality of magnetic cilia disposed on a surface of the at least one micro-channel, wherein each of the magnetic cilia has an axial direction vertical to the surface of the at least one micro-channel, and the magnetic cilia comprises:
a rotating action, wherein each of the magnetic cilia has a tilting angle with respect to the axial direction, and the magnetic cilia rotate around a rotation axis defined by the axial direction;

a magnetic field controller neighboring a first side of the receiving device and providing a magnetic field to control the rotating action;
a light projector neighboring a second side of the receiving device, wherein the light projector projects a light pattern into the receiving device, and the light pattern comprises a plurality of linear bright fringes and a plurality of linear dark fringes, both of which are alternately arranged in sequence, wherein the light pattern is a dynamic pattern, and the linear bright fringes and the linear dark fringes move along a proceeding direction, wherein the proceeding direction of the linear bright fringes and the linear dark fringes has a positive correlation with a moving direction of the living organism, wherein frequency of an appearance of the dynamic pattern ranges from 1 Hz to 1.5 Hz;
a liquid buffer circulator in communication with the receiving device; and
an image acquirer neighboring a third side of the receiving device.

2. The imaging system according to claim 1 , wherein the living organism is one of a zebrafish and a zebrafish sperm.

3. The imaging system according to claim 1 , further comprising a moving frame, wherein the receiving device is disposed on the moving frame.

4. The imaging system according to claim 1 , further comprising a flow controller electrically connected to the liquid buffer circulator.

5. The imaging system according to claim 1 , further comprising a controlling device electrically connected to one of the magnetic field controller, the light projector, and the image acquirer.

6. The imaging system according to claim 5 , wherein the controlling device further comprises a graphical user interface configured to control one of the magnetic field controller, the light projector, and the image acquirer by inputting an instruction to the graphical user interface.

Assignees

• Univ Nat Cheng Kung

Inventors

• Chen Chia-Yuan
• Mani Karthick
• Huang pin-yi
• Lu Zong-Yi

Classifications

• A61B5/0059 Primary

  using light, e.g. diagnosis by transillumination, diascopy, fluorescence (photoacoustic A61B5/0093; optical measurement of heart rate A61B5/02416; optical measurement of blood flow A61B5/0261; optical measurement of analytes A61B5/1455) · CPC title

• A61B2503/40

  Animals · CPC title

• A61D3/00 Primary

  Arrangements for supporting or fettering animals for veterinary purposes · CPC title