Human Betacoronavirus lineage C and identification of N-terminal dipeptidyl peptidase as its virus receptor

Patent metadata

Field	Value
Publication number	US-10781426-B2
Application number	US-201314430010-A
Country	US
Kind code	B2
Filing date	Sep 23, 2013
Priority date	Sep 23, 2012
Publication date	Sep 22, 2020
Grant date	Sep 22, 2020

Abstract

The invention provides an isolated essentially mammalian positive-sense single stranded RNA virus classifiable as belonging to the Order: Nidovirales; Family: Coronaviridae; Subfamily: Coronavirinae; Genus: Betacoronavirus; and non-Lineage A, non-Lineage B or non-Lineage D, human betacoronavirus. The invention also provides a human virus having a receptor binding domain (RBD) capable of binding to a dipeptidyl peptidase 4. The invention also provides diagnostic means and methods, prophylactic means and methods and therapeutic means and methods to be employed in the diagnosis, prevention and/or treatment of disease, in particular of respiratory disease, in particular of mammals, more in particular in humans.

First claim

The invention claimed is:

1. A diagnostic kit for diagnosing a MERS-CoV infection comprising:
a nucleic acid molecule at least 95% identical to the sequence of SEQ ID NO: 14, 15, 489, or 653 or a fragment thereof which can be used as a probe or primer capable of specifically hybridizing to the sequence of SEQ ID NO: 14, 15, 489, or 653 that specifically detects MERS-CoV, or
a proteinaceous molecule encoded by the nucleic acid molecule or the fragment thereof, wherein the nucleic acid molecule or the fragment thereof is detectably labelled, the detectable label selected from the group consisting of a radioisotope, a fluorophore, a quencher of fluorescence, an enzyme, an affinity tag, and combinations thereof, and
Instructions for detecting the MERS-CoV.

2. A proteinaceous substance comprising a proteinaceous molecule encoded by a nucleic acid molecule at least 95% identical to the sequence of SEQ ID NO: 14, 15, 489, or 653, and additionally comprising at least a fragment of an N-terminal dipeptidyl peptidase protein.

3. A substance according to claim 2 wherein said proteinaceous molecule comprises an ectodomain of a spike protein.

4. A substance according to claim 2 wherein said peptidase protein is a dipeptidyl peptidase 4 (DPP4).

5. A substance according to claim 2 having been subjected to crystallization.

6. A method for detecting a MERS-CoV infection, the method comprising using a method selected from southern blot or PCR with the kit of claim 1 .

Assignees

• Univ Erasmus Med Ct Rotterdam
• Dr Soliman Fakeeh Hospital

Inventors

• Haagmans Bartholomeus Leonardus
• Bestebroer Theodorus Marinus
• Van Boheemen Sander
• Fouchier Ronaldus Adrianus Maria
• Osterhaus Albertus Dominicus Marcellinus Erasmus
• Zaki Ali Moh
• Raj Victor Stalin
• Bosch Berend Jan

Classifications

• C07K16/102

  Coronaviridae (F) · CPC title

• C12N2770/20021

  Viruses as such, e.g. new isolates, mutants or their genomic sequences · CPC title

• C07K14/70596

  Molecules with a "CD"-designation not provided for elsewhere · CPC title

• C12N2770/20034

  Use of virus or viral component as vaccine, e.g. live-attenuated or inactivated virus, VLP, viral protein · CPC title

• C07K2319/30

  Non-immunoglobulin-derived peptide or protein having an immunoglobulin constant or Fc region, or a fragment thereof, attached thereto · CPC title