Microfluidic system and method of controlling particles based on artificially structured acoustic field

Patent metadata

Field	Value
Publication number	US-10780437-B2
Application number	US-201515742491-A
Country	US
Kind code	B2
Filing date	Oct 10, 2015
Priority date	Oct 10, 2015
Publication date	Sep 22, 2020
Grant date	Sep 22, 2020

Abstract

Disclosed is a microfluidic system based on an artificially structured acoustic field, comprising a microcavity used to accommodate a solution containing particles and a ultrasonic emission device used to emit ultrasound, and further comprising a phononic crystal plate placed in the microcavity. The phononic crystal plate has an artificial cycle structure, and is used to modulate the acoustic field so as to control the particles. Also disclosed is a method of controlling microfluid particles based on an artificially structured acoustic field. In the particular embodiments, since a microcavity, an ultrasonic emission device and a phononic crystal plate are comprised, the ultrasonic emission device is used to emit ultrasound, and the phononic crystal plate has an artificial cycle structure, and is used to modulate the acoustic field so as to control the particles.

First claim

What is claimed is:

1. A microfluidic system based on artificial structure tuned acoustic field, comprising:
a micro-cavity and an ultrasonic wave transmitting device arranged outside the micro-cavity, wherein the micro-cavity is configured to contain a solution containing particles, the ultrasonic wave transmitting device is configured to transmit ultrasonic waves, wherein the system further comprises a phononic crystal plate disposed in the micro-cavity, the phononic crystal plate is a periodically artificial structure and configured to modulate an acoustic field to manipulate the particles, wherein such modulating comprising:
along a designed path on the phononic crystal plate, transporting the particles based on an acoustic radiation force induced by the acoustic field modulated by the phononic crystal plate; and
capturing and aligning cells to form a cell array based on the acoustic radiation force generated by the acoustic field modulated by the phononic crystal plate, and generating a micro-vortex array to generate shear force to the cell array to induce a cell schizolysis or a regulatable sonoporation;
wherein the micro-cavity comprises an upper base, a lower base and side walls, wherein the side walls are configured to enclose an interior cavity with an opening at each end, and the upper base and the lower base are disposed at the openings respectively.

2. The system of claim 1 , wherein the phononic crystal plate comprises a substrate and a plurality of ridges disposed at a lower surface of the substrate, the ridges disposed in parallel with identical intervals.

3. The system of claim 2 , wherein the plurality of ridges comprise a fold line, a curved line or a closed loop.

4. The system of claim 3 , wherein a cross section of each of the plurality of ridges is rectangular, triangular, polygonal or semicircular.

5. The system of claim 4 , wherein the cross section of each of the plurality of ridges is a rectangle, and a distance between the central lines of the rectangle is d, a thickness of the substrate is h2, and 0.15≤h2/d≤0.25.

6. The system of claim 5 , wherein a width of the rectangle and a height of the rectangle are equal to the thickness of the substrate.

7. The system of claim 1 , wherein the ultrasonic wave transmitting device comprises a signal generator, a power amplifier, an ultrasonic transducer and an ultrasonic electrical control device, the signal generator is configured to generate a transmission signal, the power amplifier is configured to amplify the transmitted signal, the ultrasonic transducer is configured to convert an amplified signal to the ultrasonic waves, the electronic control device is configured to set parameters of the signal generator and the power amplifier, and the electronic control device is configured to switch on or switch off the ultrasonic transducer.

8. The system of claim 1 , wherein the upper base and the lower base are made of quartz glass, the side wall is made of PDMS or glass.

9. A method of manipulating particles by microfluidic based on artificial structure tuned acoustic field comprising:
placing a phononic crystal plate in a micro-cavity, wherein the phononic crystal plate is a periodically artificial structure, wherein the micro-cavity comprises an upper base, a lower base and side walls, wherein the side walls are configured to enclose an interior cavity with an opening at each end, and the upper base and the lower base are disposed at the openings respectively;
adding a solution containing particles;
transmitting ultrasonic waves by an ultrasonic wave transmitting device arranged outside the micro-cavity, and modulating an acoustic field by the phononic crystal plate; and
manipulating the particles by the phononic crystal plate based on the modulation, which comprising:
along a designed path on the phononic crystal plate, transporting the particles based on an acoustic radiation force induced by the acoustic field modulated by the phononic crystal plate; and
capturing and aligning cells to form a cell array based on the acoustic radiation force generated by the acoustic field modulated by the phononic crystal plate, and generating a micro-vortex array to generate shear force to the cell array to induce a cell schizolysis or a regulatable sonoporation.

10. The method of claim 9 , wherein the manipulating the particles by the phononic crystal plate based on the modulation comprises:
adjusting a transport path of the particles by configuring a pattern of a ridge arrangement of the phononic crystal plate; and
regulating a size of the micro-vortex array by configuring a thickness of the a substrate of the phononic crystal plate or a spacing of ridges.

11. The method of claim 9 , wherein the transmitting ultrasonic waves by an ultrasonic wave transmitting device, and modulating an acoustic field by the phononic crystal plate comprises:
when the ultrasonic wave transmitting device is configured to transmit pulse waves, a center frequency of the signal is the resonance frequency of the phononic crystal plate, and the bandwidth is 15%˜100%, and a regulation of a transport velocity is achieved by way of changing an voltage;
when the ultrasonic transmitting device is configured to transmit continuous wave, a driving frequency is configured as the resonance frequency of the phononic crystal plate to realized capturing of the particles.

12. The method of claim 9 , wherein the transmitting ultrasonic waves by an ultrasonic wave transmitting device, and modulating an acoustic field by the phononic crystal plate further comprises:
regulating the micro-vortex array quantitatively by adjusting parameters of the ultrasonic wave transmitting device to quantitatively regulate the shear force applied to the cells to control the cell perforation, or achieve a cell schizolysis or a regulatable sonoporation, wherein the parameters comprise excitation voltage, driving frequency, pulse repetition frequency and pulse duration time.

13. The method of claim 12 , wherein the transmitting ultrasonic waves by an ultrasonic wave transmitting device, and modulating an acoustic field by the phononic crystal plate further comprises:
when the ultrasonic wave transmitting device is configured for transmitting a sinusoidal pulse signal, a center frequency of the signal is the resonance frequency of the photonic crystal plate, quantitatively regulating the micro-vortex induced by the phononic crystal plate by adjusting the parameters to quantitatively regulate the shear force applied to the cells to control the cell perforation;
when the ultrasonic wave transmitting device is configured for transmitting a continuous wave, a driving frequency is configured as the resonance frequency of the phononic crystal plate, quantitatively regulating the micro-vortex induced by an artificial structure tuned acoustic field by adjusting an excitation voltage, thereby quantitatively regulate the shear force applied to the cells to control the cell perforation.

Assignees

• Shenzhen Inst Of Adv Tech Cas

Inventors

• Zheng Hairong
• Li Fei
• Cai Feiyan
• Meng Long
• Wang Chen
• Zhang Chengxiang
• Qiu Weibao
• Li Yongchuan
• Yan Fei
• Niu Lili
• Geng Liufeng
• Xu Chaowei

Classifications

• B01L3/502792

  for moving individual droplets on a plate, e.g. by locally altering surface tension · CPC title

• B01L2400/0439

  ultrasonic vibrations, vibrating piezo elements · CPC title

• B01L2200/0647

  Handling flowable solids, e.g. microscopic beads, cells, particles · CPC title

• G01N2015/1006

  for cytology · CPC title

• B01L2400/0436

  acoustic forces, e.g. surface acoustic waves [SAW] · CPC title