Multifunctional nanoparticle bioconjugates for photoacoustic-based recording of cellular membrane potential

Patent metadata

Field	Value
Publication number	US-10780185-B2
Application number	US-201816031094-A
Country	US
Kind code	B2
Filing date	Jul 10, 2018
Priority date	Jan 30, 2017
Publication date	Sep 22, 2020
Grant date	Sep 22, 2020

Abstract

A construct for detecting cellular membrane potential includes a nanoparticle operable as an electron donor; a modular peptide attached to the nanoparticle, the peptide comprising a nanoparticle association domain, a motif configured to mediate peptide insertion into the plasma membrane, and at least one attachment point for an electron acceptor positioned at a controlled distance from the nanoparticle; and an electron acceptor. The nanoparticle can be a quantum dot and the electron acceptor can be C60 fullerene. Photoacoustic emission from the construct correlates with cellular membrane potential.

First claim

What is claimed is:

1. A method of detecting cellular membrane potential, the method comprising:
providing a construct comprising a quantum dot operable as an electron donor; a modular peptide attached to the quantum dot, the peptide comprising a nanoparticle association domain, a motif configured to mediate peptide insertion into a cellular plasma membrane, and at least one attachment point for an electron acceptor positioned at a controlled distance from the quantum dot; and an electron acceptor comprising C 60 ;
contacting a cell with the construct; and
detecting a photoacoustic emission from the construct, wherein the photoacoustic emission correlates with cellular membrane potential,
wherein the modular peptide is selected from the group consisting of SEQ ID NO: 1, SEQ ID NO: 2, and SEQ ID NO: 3; and
wherein the photoacoustic emission occurs in the absence of aggregation of the construct.

2. The method of claim 1 , wherein the cell is in a tissue.

3. The method of claim 2 , wherein the tissue is in a living animal.

Assignees

• Us Gov Sec Navy

Inventors

• Delehanty James B
• Stewart Michael H
• Nag Okhil
• Deschamps Jeffrey R
• Susumu Kimihiro
• Oh Eunkeu
• Field Lauren D
• Efros Alexander L
• Huston Alan L
• Medintz Igor L
• Dawson Philip E
• Rasheed Nashaat
• Chitnis Parag V
• Cressman John R

Classifications

• G01N33/588 Primary

  with semiconductor nanocrystal label, e.g. quantum dots · CPC title

• G01N33/542

  with steric inhibition or signal modification, e.g. fluorescent quenching · CPC title

• A61K49/221

  characterised by the targeting agent or modifying agent linked to the acoustically-active agent · CPC title

• A61K49/222 Primary

  characterised by a special physical form, e.g. emulsions, liposomes · CPC title