Fluidic device, system, and method

Patent metadata

Field	Value
Publication number	US-10775368-B2
Application number	US-201715679816-A
Country	US
Kind code	B2
Filing date	Aug 17, 2017
Priority date	Mar 24, 2015
Publication date	Sep 15, 2020
Grant date	Sep 15, 2020

Abstract

A fluidic device includes: a circulation flow path; and a capture part arranged on the circulation flow path and configured to capture a sample substance in a solution and/or a detection part arranged on the circulation flow path and configured to detect a sample substance in a solution. A method of capturing a sample substance that is bound to a carrier particle, using a fluidic device which includes a circulation flow path and a capture part arranged on the circulation flow path and configured to capture the carrier particle and in which the circulation flow path has two or more circulation flow path valves, includes: an introduction step of, in a state where the circulation flow path valve is closed, introducing a solution that includes a sample substance to at least one of partitions partitioned by the circulation flow path valve and introducing a solution that includes a carrier particle which is bound to the sample substance to at least another of the partitions; a mix step of opening all of the circulation flow path valves and circulating and mixing a solution in the circulation flow path; and a capture step of capturing the carrier particle by the capture part.

First claim

What is claimed is:

1. A fluidic device, comprising:
a circulation looped flow path; and
a capture part arranged on the circulation looped flow path and configured to capture a sample substance in a solution and/or a detection part arranged on the circulation looped flow path and configured to detect a sample substance in a solution, wherein
the circulation looped flow path has two or more circulation looped flow path valves, each of the circulation looped flow path valves partitioning the circulation looped flow path into a first partition and a second partition;
a first introduction flow path is directly connected to the first partition of the circulation looped flow path;
a second introduction flow path is directly connected to the second partition of the circulation looped flow path;
a discharge flow path is connected to the circulation looped flow path;
the first introduction flow path has a first introduction flow path valve;
the second introduction flow path has a second introduction flow path valve; and
the discharge flow path has a discharge flow path valve.

2. The fluidic device according to claim 1 , wherein
the capture part captures a sample substance that is bound to a carrier particle or the detection part detects a sample substance that is bound to a carrier particle.

3. The fluidic device according to claim 2 , wherein
the capture part is configured such that an affinity with respect to the carrier particle is controllable.

4. The fluidic device according to claim 2 , wherein
a magnetic bead or a magnetic particle is used for the carrier particle, and
a magnet may be arranged in the vicinity of the capture part outside a flow path of the circulation looped flow path.

5. The fluidic device according to claim 1 , wherein
volumes of a plurality of solutions are quantitatively determined in the circulation looped flow path, and the quantitatively determined solutions are capable of being circulated and mixed.

6. The fluidic device according to claim 1 , wherein
the first introduction flow path, the second introduction flow path, and the discharge flow path are configured such that a different solution is capable of being introduced to each of the partitions of the circulation looped flow path.

7. The fluidic device according to claim 1 , wherein
at least one of the introduction flow path and the discharge flow path is arranged in the vicinity of the circulation looped flow path valve.

8. The fluidic device according to claim 1 , wherein
the circulation looped flow path has a pump by which a solution is circulated.

9. The fluidic device according to claim 8 , wherein
the pump includes at least three pump valves arranged on the circulation looped flow path.

10. The fluidic device according to claim 9 , wherein
at least one of the circulation looped flow path valves is used for the pump valve.

11. The fluidic device according to claim 1 , wherein
the capture part and the detection part are arranged at the same position of the circulation looped flow path.

12. The fluidic device according to claim 1 , further comprising:
at least one other circulation looped flow path on which a detection part that detects a sample substance in a solution is arranged, wherein
the circulation looped flow paths are connected to each other by a connection flow path.

13. A system, comprising:
a fluidic device according to claim 1 ; and a control part configured to control opening and closing of a valve.

Assignees

• Univ Tokyo
• Nikon Corp

Inventors

• Ichiki Takanori
• Ueno Taro
• Kobayashi Ryo
• Shiono Hirofumi

Classifications

• B01F33/30

  Micromixers · CPC title

• B01F25/51

  in which the mixture is circulated through a set of tubes, e.g. with gradual introduction of a component into the circulating flow · CPC title

• C12Q2565/631

  being a biochannel or pore · CPC title

• B01L2400/0605

  check valves · CPC title

• B01L2300/088

  Channel loops · CPC title