Genetic marker for determining meat quality traits of pigs and use thereof

Patent metadata

Field	Value
Publication number	US-10774381-B2
Application number	US-201715804009-A
Country	US
Kind code	B2
Filing date	Nov 6, 2017
Priority date	Nov 15, 2016
Publication date	Sep 15, 2020
Grant date	Sep 15, 2020

Abstract

The present invention relates to a genetic marker for determining meat quality traits of pigs and a use thereof, and specifically, a genetic marker for determining meat quality traits of pigs, comprising a polynucleotide consisting of 5 to 300 consecutive nucleotides comprising the nucleotides from the 1524 th to the 1527 th positions in the polynucleotide of SEQ ID NO: 1, or a polynucleotide complementary thereto, a composition for determining meat quality traits of pigs comprising an agent capable of detecting the genetic marker, a composition for determining Korean native pigs, a kit, a microarray, a method for determining meat quality traits of pigs, and a method for determining meat quality traits of Korean native pigs. The genetic marker of the present invention is a specific marker for determining the meat quality traits of pigs, and thus the marker can be used not only as a means for the objective evaluation of meat quality traits of pigs that cannot be determined by the naked eye but also as a means for distinguishing between foreign pigs and Korean native pigs, and is thereby capable of contributing to the establishment of distribution order of pork meat.

First claim

What is claimed is:

1. A method for determining a meat quality trait of a pig, comprising
(a) amplifying a polynucleotide sequence comprising nucleotides 1524-1527 of SEQ ID NO: 1, or a polynucleotide complementary thereto, from the DNA of a sample isolated from a subject pig using a primer pair consisting of nucleotide sequences of SEQ ID NOs:65 and 66;
(b) determining the presence of MYH3-1805-1810delGGACTG in the amplified polynucleotide sequence of the subject pig.

Assignees

• Republic Korea Man Rural Dev Admin
• Korea Res Inst Bioscience & Biotechnology
• Jeju National Univ Industry Academic Corporation Foundation
• Korea Res Institute Of Biosciences And Biotechnology
• Jeju National Univ Industry Academic Cooperation Foundation

Inventors

• Cho In Cheol
• Park Hee Bok
• Lee Jeong Woong
• Ahn Jin Seop
• Han Sang Hyun

Classifications

• G16B20/20 Primary

  Allele or variant detection, e.g. single nucleotide polymorphism [SNP] detection · CPC title

• C12Q1/6888 Primary

  for detection or identification of organisms · CPC title

• C12Q2600/156

  Polymorphic or mutational markers · CPC title

• A01K2227/108

  Swine · CPC title

• C12Q1/6876 Primary

  Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes · CPC title