Isolated polynucleotides and polypeptides, and methods of using same for increasing plant yield and/or agricultural characteristics

Patent metadata

Field	Value
Publication number	US-10774340-B2
Application number	US-201916401188-A
Country	US
Kind code	B2
Filing date	May 2, 2019
Priority date	Aug 27, 2013
Publication date	Sep 15, 2020
Grant date	Sep 15, 2020

Abstract

Provided are isolated polypeptides which are at least 80% homologous to SEQ ID NOs: 710-1153 and 9276-15726, isolated polynucleotides which are at least 80% identical to SEQ ID NOs: 1-709 and 1157-9275, nucleic acid constructs comprising same, transgenic cells expressing same, transgenic plants expressing same and method of using same for increasing yield, abiotic stress tolerance, growth rate, biomass, vigor, oil content, photosynthetic capacity, seed yield, fiber yield, fiber quality, fiber length, and/or nitrogen use efficiency of a plant.

First claim

What is claimed is:

1. A method of increasing yield, growth rate, biomass, seed yield, photosynthetic capacity, and/or nitrogen use efficiency of a plant, comprising transforming a plant with an exogenous polynucleotide encoding a polypeptide comprising an amino acid sequence having at least 95% amino acid sequence identity to SEQ ID NO: 1005, wherein expression of the exogenous polynucleotide encoding said polypeptide in said transformed plant increases said yield, growth rate, biomass, seed yield, photosynthetic capacity, and/or nitrogen use efficiency of the transformed plant as compared to a wild type plant of the same species grown under identical growth conditions and lacking said exogenous polynucleotide.

2. A method of increasing yield, growth rate, biomass, seed yield, photosynthetic capacity, and/or nitrogen use efficiency of a plant, comprising transforming a plant with an exogenous polynucleotide encoding a polypeptide comprising an amino acid sequence as set forth in SEQ ID NO: 1005, wherein expression of said polypeptide in said transformed plant increases said yield, growth rate, biomass, seed yield, photosynthetic capacity, and/or nitrogen use efficiency of the transformed plant as compared to a control plant of the same species grown under identical growth conditions and lacking said exogenous polynucleotide.

3. The method of claim 2 , wherein said exogenous polynucleotide comprises a nucleic acid sequence selected from the group consisting of SEQ ID NO: 680 and SEQ ID NO: 296.

4. The method of claim 1 , further comprising growing the transformed plant expressing said exogenous polynucleotide under nitrogen-limiting conditions.

5. A method of producing a transgenic crop plant, comprising growing a crop plant transformed with an exogenous polynucleotide comprising a nucleic acid sequence encoding a polypeptide comprising an amino acid sequence having at least 95% amino acid sequence identity to the amino acid sequence as set forth in SEQ ID NO: 1005, wherein the transformed crop plant is obtained from plants which have been transformed with said exogenous polynucleotide and which have been selected for increased yield, increased growth rate, increased biomass, increased seed yield, increased photosynthetic capacity, and/or increased nitrogen use efficiency as compared to a wild type plant of the same species which is grown under the same growth conditions and lacking said exogenous polynucleotide, and thereby producing the transgenic crop plant.

6. A method of producing a transgenic crop plant, comprising growing a crop plant transformed with an exogenous polynucleotide comprising a nucleic acid sequence encoding a polypeptide comprising an amino acid sequence as set forth in SEQ ID NO: 1005, wherein the transformed crop plant is obtained from plants which have been transformed with said exogenous polynucleotide and which have been selected for increased yield, increased growth rate, increased biomass, increased seed yield, increased photosynthetic capacity, and/or increased nitrogen use efficiency as compared to a wild type plant of the same species which is grown under the same growth conditions and lacking said exogenous polynucleotide, and thereby producing the transgenic crop plant.

7. The method of claim 6 , wherein said nucleic acid sequence is selected from the group consisting of SEQ ID NO: 680 and SEQ ID NO: 296.

8. A method of obtaining a transgenic crop plant, the method comprising seeding seeds and/or planting plantlets of a plant transformed with a nucleic acid construct comprising an isolated polynucleotide comprising a nucleic acid sequence encoding a polypeptide which comprises an amino acid sequence having at least 95% amino sequence identity to the amino acid sequence as set forth in SEQ ID NO: 1005, and a promoter for directing transcription of said nucleic acid sequence in a host cell, wherein said promoter is heterologous to said isolated polynucleotide and/or to said host cell, wherein expression of said amino acid sequence in said transformed plant increases yield, growth rate, biomass, photosynthetic capacity, and/or nitrogen use efficiency of said transformed plant, wherein the transformed plant is obtained from plants which have been transformed with said nucleic acid construct and which have been selected for at least one trait selected from the group consisting of: increased nitrogen use efficiency, increased biomass, increased growth rate, increased yield, and increased photosynthetic capacity as compared to a non-transformed wild type plant of the same species grown under identical growth conditions, and thereby obtaining the transgenic crop plant.

9. The method of claim 1 , further comprising selecting a plant transformed with said exogenous polynucleotide and expressing said polypeptide and exhibiting increase in yield, growth rate, biomass, photosynthetic capacity, and/or nitrogen use efficiency as compared to the wild type plant of the same species which is grown under the same growth conditions and lacking said exogenous polynucleotide.

10. The method of claim 9 , wherein said selecting is performed under non-stress conditions.

11. The method of claim 9 , wherein said selecting is performed under nitrogen-limiting stress conditions.

12. The method of claim 8 , wherein said amino acid sequence is set forth in SEQ ID NO: 1005.

Assignees

• Evogene Ltd

Inventors

• Gil Lidor
• Rimon Knopf Ronit
• Matarasso Noa
• Panik David
• Poraty-Gavra Limor
• Katz Ehud
• Karchi Hagai

Classifications

• Y02A40/146

  Genetically Modified [GMO] plants, e.g. transgenic plants · CPC title

• C12N15/8261 Primary

  with agronomic (input) traits, e.g. crop yield · CPC title

• C12N15/8247

  involving modified lipid metabolism, e.g. seed oil composition · CPC title

• C07K14/415

  from plants · CPC title

• C12N15/8273

  for drought, cold, salt resistance · CPC title