Multi-modal optical imaging system for tissue analysis

Patent metadata

Field	Value
Publication number	US-10768114-B2
Application number	US-201615501727-A
Country	US
Kind code	B2
Filing date	Apr 29, 2016
Priority date	Apr 29, 2016
Publication date	Sep 8, 2020
Grant date	Sep 8, 2020

Abstract

A modular system for organic sample analysis is disclosed which includes a sample stage including a support platform and a motorized positioning mechanism mounted on the support platform, and a sample holder mounted on the motorized positioning mechanism upon which a sample is placed. A probe support rack is mounted on the support platform and two or more bio-imaging probes mounted on the probe support and arranged in a pre-defined geometry with respect to each other, and at least one bio-imaging probe has a field of view independent of all other bio-imaging probes. The system includes a computer controller connected to the motorized positioning mechanism and the two or more bio-imaging probes. The computer is programmed for controlling motorized positioning mechanism to move the sample holder having the sample located thereon to positions in the field of view of each bio-imaging probe where the sample can be analyzed individually by each of the bio-imaging probes. The computer includes a storage medium for storing an imaging data from each bio-imaging probe. The computer is programmed for spatially correlating imaging data of the selected volume of interest with the imaging data obtained from at least one other bio-imaging probe and storing the spatially correlated imaging data in the computer storage medium.

First claim

What is claimed is:

1. A modular system for organic sample analysis of tissue, comprising:
a sample stage comprising a support platform, a motorized positioning assembly mounted on said support platform, the support platform comprising a vibrationally damped base, the vibrationally damped base preventing noise in an optical signal, and a sample holder mounted on said motorized positioning assembly upon which a tissue sample is placed, the motorized positioning assembly comprising a motorized stage driver and at least one actuator, the at least one actuator comprising at least one of: a motorized stage actuator, a DC motor, a stepper motor, and a piezoelectric effect actuator, the motorized stage driver providing necessary electrical driving signals for the motorized stage actuators and collecting electrical signals from at least one sensor, and the at least one sensor providing information relating to a state of the motorized positioning assembly;
a probe support rack mounted on said support platform;
a plurality of bio-imaging probes mounted on said probe support rack with said bio-imaging probes arranged in a pre-defined geometry with respect to one another, at least one bio-imaging probe having a field of view independent of all other bio-imaging probes;
a computer controller coupled with said motorized positioning mechanism assembly and said plurality of bio-imaging probes, said computer controller programmed with instructions for instructing said motorized positioning assembly to move said sample holder, having the tissue sample located thereon, to positions in the field of view of each bio-imaging probe where the tissue sample is individually analyzable by each of said bio-imaging probes, said computer controller comprising means for storing an imaging data from each said bio-imaging probe, and said computer controller programmed with instructions for spatially correlating imaging data of a selected volume of interest with the imaging data obtained from at least one other bio-imaging probe and storing said spatially correlated imaging data in said storing means;
an enclosure accommodating the plurality of bio-imaging probes and motorized positioning assembly; and
a ventilation system configured to circulate fresh air into the enclosure.

2. The modular system according to claim 1 , wherein said computer controller comprises a user interface to facilitate a user obtaining, using a designated bio-imaging probe, imaging data of the tissue sample to be visually displayed and used by means for selecting a volume of interest of the tissue sample to be imaged by said at least one additional bio-imaging probe.

3. The modular system according to claim 2 , wherein said user interface is configured to allow a user to select a single volume of a tissue sample to be probed by the plurality of bio-imaging probes, or to allow a user to select multiple volumes independent of one another to be probed by the plurality of bio-imaging probes.

4. The modular system according to claim 2 , wherein said computer controller comprises a plurality of pre-defined algorithms that facilitate sample analysis by executing a pre-determined sequence of selections of sample volumes of interest, sample positioning steps, data acquisitions by the bio-imaging probes, and data analysis.

5. The modular system according to claim 2 ,
wherein said computer controller comprises a plurality of machine learning algorithms that facilitates sample analysis by making choices of selections of sample volumes of interest, sample positioning steps, data acquisitions by the bio-imaging probes, and imaging data analysis, and wherein the choices are based on information about the tissue samples previously acquired.

6. The modular system according to claim 4 , wherein said computer controller comprises a plurality of pre-defined algorithms that perform sample analysis by executing pre-determined sequence of selections of the sample volumes of interest, sample positioning steps, data acquisitions by the bio-imaging probes, and data analysis.

7. The modular system according to claim 6 ,
wherein said computer controller additionally comprises a plurality of machine learning algorithms that facilitates sample analysis by making choices of selections of the sample volumes of interest, sample positioning steps, data acquisitions by the bio-imaging probes, and imaging data analysis, and
wherein the choices are based on information about the tissue sample previously acquired.

8. The modular system according to claim 4 ,
wherein said computer controller is coupled with a distant computer system,
wherein said distant computer system comprises a user interface, and
wherein said user interface is operable by a distant user operating the computer controller within the modular system,
whereby the sequence of selections of the sample volumes of interest, sample positioning steps, data acquisitions by the bio-imaging probes, and data analysis are controllable.

9. The modular system according to claim 1 ,
wherein said computer controller is coupled with a distant computer system, and
wherein said distant computer system comprises a plurality of pre-defined algorithms that facilitate sample analysis by controlling the computer controller within the modular system,
whereby the sequence of selections of the sample volumes of interest, sample positioning steps, data acquisitions by the bio-imaging probes, and data analysis are controllable.

10. The modular system according to claim 1 ,
wherein said computer controller is connected to a distant computer system,
wherein said distant computer system comprises a plurality of machine learning algorithms that facilitates sample analysis by controlling the computer controller within the modular system by making choices of selections of the sample volumes of interest, sample positioning steps, data acquisitions by the bio-imaging probes, and imaging data analysis, and
wherein the choices are based on information about the tissue sample previously acquired.

11. The modular system according to claim 2 , wherein said computer controller is programmed with instructions to assign imaging data acquired at a certain point in the field of view of a selected bio-imaging probe to visually displayed imaging data of the designated probe.

12. The modular system according to claim 2 ,
wherein said designated bio-imaging probe comprises a 3D scanner, and
wherein said computer controller is programmed with instructions to display a 3D profile on a two dimensional computer display.

13. The modular system according to claim 1 ,
wherein said designated bio-imaging probe comprises a 3D scanner, and
wherein said computer controller is programmed with instructions to display a 3D profile on a three dimensional computer display.

14. The modular system according to claim 12 ,
wherein said 3D profile comprises a surface profile of a tissue sample, and
wherein said surface profile of the tissue sample is stored by a control computer as a set of coordinates relative to said 3D scanner.

15. The modular system according to claim 12 , wherein operation of said 3D scanner comprises one system of: a time-of-flight system, a triangulation system, a structured light system, a modulated light system, a stereoscopic system, and a photometric system.

16. The modular system according to claim 2 , wherein said designated bio-imaging probe comprises one of a visible light camera and an infrared camera.

17. The modular system according to claim 16 , further comprising a pair of lasers, each laser of the pair of laser

Assignees

• Synaptive Medical Barbados Inc

Inventors

• Franjic Kresimir
• Mak Siu Wai Jacky
• Hynna Kai Michael
• Wood Michael Frank Gunter
• Kuchnio Piotr
• Bismilla Yusuf
• Holmes Lachlan Noel
• Bright Stewart
• Cheung Aaron Yu Lai
• Kuzyk Yuri Alexander
• Taub Aryeh Benjamin
• Rezaei Sanaz

Classifications

• G01N21/6456

  Spatial resolved fluorescence measurements; Imaging · CPC title

• G01N2021/653

  Coherent methods [CARS] · CPC title

• G01N21/31

  Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry {(G01N21/72 takes precedence)} · CPC title

• G01B9/02091

  Tomographic interferometers, e.g. based on optical coherence · CPC title

• G01N21/65 Primary

  Raman scattering · CPC title