Fiber sampler for recovery of bioaerosols and particles

Patent metadata

Field	Value
Publication number	US-10767210-B2
Application number	US-201916455067-A
Country	US
Kind code	B2
Filing date	Jun 27, 2019
Priority date	Feb 17, 2012
Publication date	Sep 8, 2020
Grant date	Sep 8, 2020

Abstract

An aerosol collection system and method. The system includes a bio-aerosol delivery device configured to supply bioparticles in a gas stream, a moisture exchange device including a partition member coupled to the gas stream and configured to humidify or dehumidify the bioparticles in the gas stream, and an aerosol collection medium downstream from the moisture exchange device and configured to collect the bioparticles. The method includes delivering bioparticles in a gas stream, humidifying or dehumidifying the bioparticles in the gas stream by transport of water across a partition member and into a vapor phase of the gas stream, and collecting the bioparticles by a collection medium.

First claim

The invention claimed is:

1. A method for collecting aerosols, comprising:
humidifying or dehumidifying bioparticles in a first gas stream by transport of water vapor a) between an exterior side of a partition member and an interior side of the partition member and b) into a vapor phase of a second gas stream including the bioparticles;
delivering the bioparticles into the second gas stream;
controlling a pressure of the first gas stream on the exterior side of the partition member to adjust a relative humidity for the bioparticles in the second gas stream; and
collecting the bioparticles by a collection medium.

2. The method of claim 1 , wherein humidifying or dehumidifying the particles comprises maintaining a relative humidity greater than 5%, greater than 10%, greater than 15%, greater than 25%, or greater than 50%.

3. The method of claim 2 , wherein humidifying or dehumidifying the particles comprises transporting the water across the partition member comprising at least one of:
a copolymer of perfluoro-3,6-dioxa-4-methyl-7octene-sulfonic acid and tetrafluoroethylene;
polyethylene oxide, polyvinyl alcohol, cellulose ether and starch) and copolymers thereof; and
polyacrylic acid, polyacrylamide, polyisopropyl acrylamide, polystyrene sulfonic acid, polyvinyl pyridine and polyamino acid and copolymers and salts thereof.

4. The method of claim 1 , wherein humidifying or dehumidifying the particles comprises transporting the water vapor across the partition member comprising at least one of a permeable material, a semi-permeable membrane material, or a polymeric ionomer.

5. The method of claim 1 , further comprising:
stabilizing at least one of a temperature and relative humidity of the aerosol collection medium prior to collecting the bioparticles.

6. The method of claim 1 , further comprising:
controlling a temperature and a relative humidity along a gas-flow axis of the membrane.

7. The method of claim 6 , further comprising:
adjusting the temperature and the relative humidity such that the particles in the second gas stream transitions from outside ambient conditions to a target temperature and relative humidity condition.

8. The method of claim 7 , further comprising:
adjusting the temperature and the relative humidity to at least one of:
2° C. to 10° C. and RH of 5 to 95%
2° C. to 10° C. and RH of 70 to 85%;
1° C. to 8° C. and RH of 5 to 95%;
1° C. to 8° C. and RH of 70 to 85%;
2° C. to 6° C. and RH of 5 to 95%;
2° C. to 6° C. and RH of 70 to 85%;
4° C. and RH of 5 to 95%; or
4° C. and RH of 70 to 85%.

9. The method of claim 7 , further comprising:
adjusting a temperature and a relative humidity of the bioparticle while transitioning from outside ambient conditions to the target temperature and relative humidity condition along a controlled path of temperature and humidity,
said controlled path comprises a two-stage system including a first stage having a relative humidity in a range from 40-60% at ambient temperature and a second stage below ambient temperature while maintaining the relative humidity in said range from 40-60%.

10. The method of claim 1 , further comprising:
providing a viability enhancing material to the collection medium.

11. The method of claim 1 , wherein providing a viability enhancing material comprises providing at least one of water, proteins, carbohydrates, sugars, salts, phosphate buffered saline, and tryptic soy broth to the collection medium.

12. The method of claim 1 , wherein collecting the bioparticles comprises collecting at least one of pox viruses, filoviruses, arenaviruses, alphavirus, Brucella species, Burkholderia mallei, Yersinia pestis and Coxiella burnetii on the collection medium.

13. The method of claim 1 , wherein the partition member prevents contamination of the bioparticles in the second gas stream from contaminants in the first gas stream.

14. The method of claim 1 , wherein the partition member comprises a tube in shell device preventing contamination.

Assignees

• Res Triangle Inst
• Res Triangle Inc

Inventors

• Ensor David S
• Walls Howard Jerome
• Foarde Karin K
• Hering Susanne Vera
• Spielman Steven Russel

Classifications

• G01N2001/2217

  using a liquid · CPC title

• C12Q1/24 Primary

  Methods of sampling, or inoculating or spreading a sample; Methods of physically isolating an intact microorganisms · CPC title

• G01N1/2202 Primary

  involving separation of sample components during sampling · CPC title