Optical cavity PCR

Patent metadata

Field	Value
Publication number	US-10766034-B2
Application number	US-201815878033-A
Country	US
Kind code	B2
Filing date	Jan 23, 2018
Priority date	Jul 30, 2015
Publication date	Sep 8, 2020
Grant date	Sep 8, 2020

Abstract

An LED-driven optical cavity PCR system and method is disclosed for fast, accurate and reliable PCR based diagnostics. An optical cavity comprising two thin light absorbing metal (AU) films is used for uniform light absorption and subsequent photo thermal light-to-heat conversion is employed for PCR thermal cycling.

First claim

What is claimed is:

1. A method for performing ultra fast thermal cycling of a fluidic sample, the method comprising:
providing a micro-fluidic thermal cycling chamber defined by opposing first and second thin films;
filling the micro-fluidic thermal cycling chamber with the fluidic sample;
illuminating the first thin film with a light source;
wherein a first portion of light illuminated onto the first thin film is absorbed into the first thin film and a second portion of the light illuminated onto the first thin film is transmitted through the first thin film;
illuminating the second thin film with the light transmitted through the first thin film;
wherein at least a portion of the transmitted light illuminating the second thin film is absorbed into the second thin film;
uniformly elevating a temperature of the first thin film and a temperature of the second thin film as a function of the absorbed light into the first thin film and second thin film; and
heating the fluidic sample within the micro-fluidic thermal cycling chamber as a result of the elevated temperature of the first thin film and second thin film.

2. The method of claim 1 , wherein illumination of the first thin film is intermittently applied to perform ultra fast micro-fluidic polymerase chain reaction (PCR) of the fluidic sample.

3. The method of claim 2 , wherein uniformly elevating the temperature of the first thin film and second thin film comprises:
illuminating the first and second thin films for a first duration to raise the temperature of the fluid sample in the micro-fluidic thermal cycling chamber to a selected temperature for a first period;
illuminating the first and second thin films for a second duration to raise the temperature of the fluid sample in the micro-fluidic thermal cycling chamber to a selected temperature for a second period;
illuminating the first and second thin films for a third duration to raise the temperature of the fluid sample in the micro-fluidic thermal cycling chamber to a selected temperature for a third period; and
repeating a cycle of illumination periods for multiple cycles to amplify the fluid sample.

4. The method of claim 2 , wherein the first thin film has a first thickness, and the second thin film has a second thickness different than the first thickness.

5. The method of claim 4 , wherein the first thin film thickness and second thin film thickness are selected so as to match a rate of absorption of light into the first thin film and second thin film such that the first thin film and second thin film have a substantially uniform rate of temperature elevation.

6. The method of claim 2 , further comprising:
measuring a temperature within the micro-fluidic thermal cycling chamber.

7. The method of claim 2 , wherein the first thin film and second thin film have a surface covered with a passivation layer to prevent PCR reaction inhibition within the micro-fluidic thermal cycling chamber.

8. The method of claim 2 , wherein filling the micro-fluidic thermal cycling chamber with the fluidic sample comprises:
injecting fluidic sample into the cycling chamber through a first port coupled to the micro-fluidic thermal cycling chamber;
wherein the injected fluid sample pushes air out of a second port coupled to the micro-fluidic thermal cycling chamber.

9. The method of claim 2 , wherein the micro-fluidic thermal cycling chamber is configured for lasing of fluorescent emission during the PCR reaction.

Assignees

• Univ California

Inventors

• Lee Luke P
• Son Jun Ho

Classifications

• B01L2300/1861

  using radiation · CPC title

• B01L2300/0893

  having a very large number of wells, microfabricated wells · CPC title

• B01L7/52 Primary

  with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples · CPC title

• B01L3/502707

  characterised by the manufacture of the container or its components · CPC title

• B01L2300/1827

  using resistive heater · CPC title