Test apparatus and target measurement method using the same

Patent metadata

Field	Value
Publication number	US-10761101-B2
Application number	US-201715683365-A
Country	US
Kind code	B2
Filing date	Aug 22, 2017
Priority date	Jan 9, 2013
Publication date	Sep 1, 2020
Grant date	Sep 1, 2020

Abstract

A test apparatus and method for measuring a concentration of a target by correcting for an impact of hemoglobin are provided. The target measurement method includes measuring an absorbance of hemoglobin in a sample, measuring an absorbance of a target in the sample, determining variation of the absorbance of the target according to the measured absorbance of the hemoglobin, and correcting the absorbance of the target by subtracting the determined variation of the absorbance of the target from the measured absorbance of the target.

First claim

What is claimed is:

1. A target measurement method comprising:
measuring an absorbance of hemoglobin in a sample;
measuring an absorbance of a target in the sample;
determining a variation of the absorbance of the target according to the measured absorbance of the hemoglobin; and
correcting the absorbance of the target by subtracting the determined variation of the absorbance of the target from the measured absorbance of the target,
wherein the determining the variation of the absorbance of the target comprises determining the variation of the absorbance of the target according to the measured absorbance of the hemoglobin based on a pre-determined correlation showing the variation of the absorbance of the target according to the absorbance of the hemoglobin.

2. The target measurement method according to claim 1 , wherein the measuring the absorbance of hemoglobin comprises:
irradiating the sample with light to measure an absorbance of the sample; and
determining the absorbance of hemoglobin in the sample based on the measured absorbance of the sample and a pre-determined equation for calculation of the absorbance of the hemoglobin.

3. The target measurement method according to claim 2 , wherein the irradiating the sample comprises irradiating a sample accommodated in a chamber of a reactor, excluding a reagent for detection of the target, with light having at least one predetermined wavelength.

4. The target measurement method according to claim 3 , wherein the reactor comprises:
at least one reaction chamber; and
a control chamber excluding the reagent.

5. The target measurement method according to claim 2 , wherein the determining the absorbance of the hemoglobin comprises determining the absorbance of the hemoglobin based on the pre-determined equation for calculation of the absorbance of the hemoglobin independently from change in concentration of the target, when measurement results of the absorbance of the sample are input.

6. The target measurement method according to claim 4 , wherein the pre-determined equation for calculation of the absorbance of the hemoglobin comprises a variable to be substituted with the absorbance obtained by irradiating the sample accommodated in the control chamber with the light having the at least one predetermined wavelength and a coefficient for calculation of the absorbance of the hemoglobin independently from change in concentration of the target.

7. The target measurement method according to claim 1 , wherein the measuring the absorbance of the target comprises measuring the absorbance of the target in the sample by irradiating the sample accommodated in a chamber of a reactor, containing a reagent for detection of the target, with light having at least one predetermined wavelength.

8. The target measurement method according to claim 1 , further comprising determining a concentration of the target based on the corrected absorbance of the target after the absorbance of the target is corrected.

9. The target measurement method according to claim 1 , wherein the sample comprises blood plasma or serum.

10. The target measurement method according to claim 3 , wherein a wavelength of light irradiated to the sample is at least one of 450 nm, 535 nm, and 630 nm.

11. The target measurement method according to claim 3 , wherein the irradiating the sample with the light to measure the absorbance of the sample comprises:
determining a wavelength of light such that change in the absorbance of the hemoglobin according to change in concentration of the target is minimized.

12. The target measurement method according to claim 10 , wherein the pre-determined equation for calculation of the absorbance of the hemoglobin determines a coefficient such that change in the absorbance of the hemoglobin according to change in concentration of the target is minimized.

13. The target measurement method according to claim 1 , wherein the correcting the absorbance of the target comprises:
obtaining the variation of the absorbance of the target according to a concentration of the hemoglobin based on the pre-determined correlation between the absorbance of the hemoglobin and the absorbance of the target; and
correcting the absorbance of the target by subtracting the determined variation of the absorbance of the target from the measured absorbance of the target based on the variation of the absorbance of the target according to the concentration of the hemoglobin.

Assignees

• Samsung Electronics Co Ltd

Inventors

• Lee Sung Hwa
• Taguchi Takayuki
• Park Hye Kyung

Classifications

• A61B5/14

  Devices for taking samples of blood (hypodermic syringes A61M5/00); Measuring characteristics of blood in vivo, e.g. gas concentration within the blood, pH-value of blood (measuring of blood pressure A61B5/02; non-radiation detecting or locating of foreign bodies in blood A61B5/06) · CPC title

• G01N33/72

  involving blood pigments, e.g. haemoglobin, bilirubin {or other porphyrins; involving occult blood} · CPC title

• G01N33/52 Primary

  Use of compounds or compositions for colorimetric, spectrophotometric or fluorometric investigation, e.g. use of reagent paper {and including single- and multilayer analytical elements (immunological elements G01N33/54386; involving labelled immunochemicals G01N33/58; for haemoglobin or occult blood G01N33/72)} · CPC title

• G01N33/726

  Devices · CPC title

• G01N33/721 Primary

  Haemoglobin · CPC title